Vascularisation in regenerative therapeutics and surgery

Jeyaraj, Rebecca; Natasha, G; Kirby, Georgia; Rajadas, Jayakumar; Mosahebi, Afshin; Seifalian, Alexander M.; Tan, Aaron

doi:10.1016/j.msec.2015.05.045

Cited by 10 publications

(15 citation statements)

References 68 publications

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“…It has been found that the location of cells more than 200 μm away from the nearest capillaries will undergo hypoxia, apoptosis and death, due to the internal mass transfer limitations [120]. Therefore, within the 3D bioprinted tissues or organs, implement of vascularization is crucial for penetrations of oxygen and nutrients as well as long-term maintenance of tissue functions [121]. The vascularization will greatly contribute to the solution of maintaining high cell viability and functionality in 3D bioprinted complex and large-volume tissue constructs, but there's still a long way to go.…”

Section: Vascularizationmentioning

confidence: 99%

3D bioprinting for cell culture and tissue fabrication

Jian

Wang

et al. 2018

Bio-des. Manuf.

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Three-dimensional (3D) bioprinting is a computer-assisted technology which precisely controls spatial position of biomaterials, growth factors and living cells, offering unprecedented possibility to bridge the gap between structurally mimic tissue constructs and functional tissues or organoids. We briefly focus on diverse bioinks used in the recent progresses of biofabrication and 3D bioprinting of various tissue architectures including blood vessel, bone, cartilage, skin, heart, liver and nerve systems. This paper provides readers a guideline with the conjunction between bioinks and the targeted tissue or organ types in structuration and final functionalization of these tissue analogues. The challenges and perspectives in 3D bioprinting field are also illustrated.

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Section: Vascularizationmentioning

confidence: 99%

3D bioprinting for cell culture and tissue fabrication

Jian

Wang

et al. 2018

Bio-des. Manuf.

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“…Therefore most tissues, particularly complex tissues and tissues with high metabolic demand, will mandate vascularization (or connection/anastomosis to recipient systemic circulation). This will ensure the developing bioengineered tissue will receive adequate oxygen and nutrients from the recipient, and adequate removal of harmful metabolites, to maintain viability [95,96]. Vascularity also allows thicker and denser tissues to be constructed, and for innervation and lymphangiogenesis within the tissue [95].…”

Section: Vascularizationmentioning

confidence: 99%

“…This will ensure the developing bioengineered tissue will receive adequate oxygen and nutrients from the recipient, and adequate removal of harmful metabolites, to maintain viability [95,96]. Vascularity also allows thicker and denser tissues to be constructed, and for innervation and lymphangiogenesis within the tissue [95]. Two means of achieving vascularization include vasculogenesis (de novo formation of blood vessels from epithelial progenitor cells) and angiogenesis (vascular expansion by growing blood vessels from pre-existing blood vessels) [96,97].…”

Section: Vascularizationmentioning

confidence: 99%

“…Ex vivo approaches include spheroid coculture, cell sheet engineering, scaffold functionalization, modular assembly, and bioprinting. In vivo approaches include polysurgery, arteriovenous loops, and genetic manipulations [95]. Hooper et al demonstrated that hydrogel scaffolds could be vascularized via endothelialized microchannels in an in vivo rat model, thus overcoming a major obstacle of synthetic constructs [98 • ].…”

Section: Vascularizationmentioning

confidence: 99%

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Whole-Organ Tissue Engineering: No Longer Just a Dream

Wrenn

Weiss

2016

Curr Pathobiol Rep

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Purpose of Review The true potential of the field of transplant surgery remains limited due to shortages of available transplantable allografts and, following transplantation, acute and chronic rejection with need for lifelong immune suppression. An alternative approach is to bioengineer organs to be utilized in vivo, replacing diseased or malfunctioning human organs. This revolutionary step in medicine could have virtually unlimited therapeutic potential. Recent Findings Multiple strategies have been used to replicate functional transplantable organs without deleterious host immune response. Common approaches include the use of decellularized tissue scaffolds and of 3D bioprinting. Continuing challenges include engraftment and maturation of recipient cells, and long-term tissue viability and function. Summary We will review in brief the history of the field and the progress to date with multiple organ systems, highlighting our personal experience in lung regeneration. Finally, we will discuss the challenges that lie ahead to reach the dream of fully functional and transplantable bioengineered organs.

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“…7,8 However, vascularization remains one of the great challenges for tissue engineering limiting its potential to grow tissues of clinically relevant size. 9 Current approaches to vascularize tissue follow either an extrinsic pathway where new vessels grow from the recipient vascular bed and invade throughout the implanted tissue constructs 10 or an intrinsic vascularization pathway where the vasculature grows and expands in unison with the newly developing tissue. 11 The extrinsic approach traditionally involves seeding cells onto a scaffold in vitro and implanting the complete construct into the living animal with the expectation that nutrients, previously supplied by culture media, will be sourced from the circulation.…”

Section: Introductionmentioning

confidence: 99%

Tissue Engineering by Intrinsic Vascularization in an <em>In Vivo</em> Tissue Engineering Chamber

Zhan

Marré

Mitchell

et al. 2016

JoVE

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In reconstructive surgery, there is a clinical need for an alternative to the current methods of autologous reconstruction which are complex, costly and trade one defect for another. Tissue engineering holds the promise to address this increasing demand. However, most tissue engineering strategies fail to generate stable and functional tissue substitutes because of poor vascularization. This paper focuses on an in vivo tissue engineering chamber model of intrinsic vascularization where a perfused artery and a vein either as an arteriovenous loop or a flow-through pedicle configuration is directed inside a protected hollow chamber. In this chamber-based system angiogenic sprouting occurs from the arteriovenous vessels and this system attracts ischemic and inflammatory driven endogenous cell migration which gradually fills the chamber space with fibro-vascular tissue. Exogenous cell/matrix implantation at the time of chamber construction enhances cell survival and determines specificity of the engineered tissues which develop. Our studies have shown that this chamber model can successfully generate different tissues such as fat, cardiac muscle, liver and others. However, modifications and refinements are required to ensure target tissue formation is consistent and reproducible. This article describes a standardized protocol for the fabrication of two different vascularized tissue engineering chamber models in vivo.

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Vascularisation in regenerative therapeutics and surgery

Cited by 10 publications

References 68 publications

3D bioprinting for cell culture and tissue fabrication

3D bioprinting for cell culture and tissue fabrication

Whole-Organ Tissue Engineering: No Longer Just a Dream

Tissue Engineering by Intrinsic Vascularization in an <em>In Vivo</em> Tissue Engineering Chamber

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