Vascularisation in adipose depots surrounding immune‐stimulated lymph nodes

MacQueen, Hilary; Waights, Verina; Pond, Caroline M.

doi:10.1046/j.1469-7580.1999.19410033.x

Cited by 14 publications

(10 citation statements)

References 17 publications

(14 reference statements)

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“…8 Immunocytochemistry with anti-rat mononuclear phagocyte in the adipose tissue of tumour-bearing rats (A) and control reaction (B) studies found no variation between the constitutive secretion (Eriksson et al 2000) or gene expression (Montague et al 1997) between these latter depots. However, a greater expression of TNF receptors was found in the mesenteric adipocytes adjacent to lymph nodes (Macqueen et al 1999). We were not able to find studies on the heterogeneity of TNFα secretion by adipose tissue during cachexia, and hence we conclude that this is the first attempt to characterize interdepot variation in this parameter.…”

Section: Discussionmentioning

confidence: 52%

Adipose tissue in Walker 256 tumour-induced cachexia: possible association between decreased leptin concentration and mononuclear cell infiltration

2004

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The adipose tissue (AT) is severely affected by cachexia, a paraneoplastic syndrome, which increases the morbidity and mortality of cancer. There is, however, a heterogeneous response to the condition, according to the AT depot. As plasma leptin concentration has been often reported to vary in cachexia, we have measured (species specific radioimmunoassay) the local concentration of leptin in three AT depots: retroperitoneal (RPAT), epididymal (EAT) and mesenteric (MES) of Walker 256 tumour-bearing rats. A reduced concentration of leptin ( P<0.0001) was found in all the depots and in the plasma of the cachectic rats, compared with controls already from day 4 after tumour cell injection. The presence of a cell infiltrate was observed in all AT obtained from the tumour-bearing animals. Ultrastructural analysis, along with immunocytochemistry for RT1B (indicating the presence of MHCII) and using antibody against mononuclear phagocytes, showed the cells to be macrophages. The profile of TNFalpha and PGE2 secretion by the infiltrate was investigated (commercial kits). There was increased production of both factors by the cells of all AT ( P<0.05) compared with peritoneal macrophages obtained from the cachectic rats, while the cells isolated from MES showed the highest synthesis of TNFalpha. The results suggest a possible modulation of the chronic locally produced TNFalpha and PGE2 upon leptin synthesis by the AT of the cachectic rats.

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Section: Discussionmentioning

confidence: 52%

Adipose tissue in Walker 256 tumour-induced cachexia: possible association between decreased leptin concentration and mononuclear cell infiltration

2004

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“…The results are not consistent with the long-established assumption (Clandinin et al 1991) that the precursors of PL of newly formed cells are derived directly from the diet via blood-borne lipids. Vascularisation of the perinodal adipose tissue increases under chronic immune stimulation, but the process is very slow compared with the timescale of this experiment (MacQueen et al 1999). Increased perfusion of both the inflamed lymph node and its surrounding adipose tissue would obliterate most of the changes induced by the supplemented diets in the inflamed tissues, replacing them with fatty acids derived from the plain diet.…”

Section: Paracrine Relationship Between Adipose and Lymphoid Tissuesmentioning

confidence: 86%

The source of fatty acids incorporated into proliferating lymphoid cells in immune-stimulated lymph nodes

Pond

Mattacks

2003

Br J Nutr

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To explore the hypothesis that proliferating lymphoid cells in immune-stimulated lymph nodes obtain nutrients locally from adjacent adipose tissue, adult guinea pigs were fed for 6 weeks on standard chow or on chow supplemented with 100 g suet, sunflower oil or fish oi/g. All the guinea pigs ate standard chow for the last 5 d, during which swelling of one popliteal lymph node was stimulated by repeated local injection of lipopolysaccharide. The fatty acid compositions of phospholipids in both popliteal and in several mesenteric lymph nodes, and of triacylglycerols in eleven samples of adipose tissue defined by their anatomical relations to lymph nodes, were determined by GC. The proportions of fatty acids in the phospholipids extracted from the stimulated popliteal node correlated best with those of triacylglycerols in the surrounding adipocytes, less strongly with those of adipocytes elsewhere in depots associated with lymphoid tissue, but not with those of nodeless depots. The composition of triacylglycerols in the perinodal adipose tissue changed under local immune stimulation. We conclude that proliferating lymphoid cells in activated lymph nodes obtain fatty acids mainly from the triacylglycerols in adjacent perinodal adipose tissue. Immune stimulation prompts changes in the fatty acid composition of the triacylglycerols of adipocytes in node-containing depots that equip the adipose tissue for provisioning immune responses. Such local interactions show that specialised adipocytes can act as an interface between whole-body and cellular nutrition, and may explain why mammalian adipose tissue is partitioned into a few large and many small depots.

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“…1994; Schling & Löffler, 2002). Vascularization of perinodal adipose tissue increases slowly during chronic inflammation (MacQueen et al. 1999).…”

Section: Discussionmentioning

confidence: 99%

The cellular structure and lipid/protein composition of adipose tissue surrounding chronically stimulated lymph nodes in rats

2003

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To test the hypothesis that chronic immune stimulation of a peripheral lymph node induces the formation of additional mature adipocytes in adjacent adipose tissue, one popliteal lymph node of large male rats was stimulated by local injection of 10 µg or 20 µg lipopolysaccharide three times a week for 6 weeks. Adipocyte volumes in sites defined by their anatomical relations to the stimulated and homologous unstimulated popliteal lymph nodes were measured, plus adipocyte complement of the popliteal depot, and the lipid and protein content of adipocytes and adipose stroma. The higher dose of lipopolysaccharide doubled the mass of the locally stimulated lymph node and the surrounding adipose tissue enlarged by the appearance of additional mature adipocytes. Similar but smaller changes were observed in the popliteal adipose depot of the unstimulated leg and in a nodeless depot. The lipid content of the adipocytes decreased and that of the stroma increased dose-dependently in all samples measured

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Vascularisation in adipose depots surrounding immune‐stimulated lymph nodes

Cited by 14 publications

References 17 publications

Adipose tissue in Walker 256 tumour-induced cachexia: possible association between decreased leptin concentration and mononuclear cell infiltration

Adipose tissue in Walker 256 tumour-induced cachexia: possible association between decreased leptin concentration and mononuclear cell infiltration

The source of fatty acids incorporated into proliferating lymphoid cells in immune-stimulated lymph nodes

The cellular structure and lipid/protein composition of adipose tissue surrounding chronically stimulated lymph nodes in rats

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