Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles

Kang, Kyeongah; Lím, D.; Choi, In Hong; Kang, Taegyeong; Lee, Kangtaek; Moon, Eun Yi; Yang, Young; Lee, Myeong Sok; Lim, Jong Seok

doi:10.1016/j.toxlet.2011.05.1033

Cited by 87 publications

(84 citation statements)

References 36 publications

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“…Moreover, Ag NP were anti-angiogenic when evaluated in chick chorioallantoic membranes and in matrigel plugs in mice [27] . At the moment, we are aware of a single report demonstrating that Ag NP induce angiogenesis [11] . These discordant findings might result from the use of a transformed endothelial cell line [11] .…”

Section: Discussionmentioning

confidence: 99%

“…At the moment, we are aware of a single report demonstrating that Ag NP induce angiogenesis [11] . These discordant findings might result from the use of a transformed endothelial cell line [11] . While cell lines are useful for some experiments, they appear to be less suited for studies focused on the regulation of cell survival, proliferation and apoptosis [28] .…”

Section: Discussionmentioning

confidence: 99%

“…They also reduce VEGF and interleukin 1β-induced permeability in porcine retinal endothelial cells [10] . In contrast with these studies, Kang et al [11] showed that Ag NP stimulated the murine endothelial cell line SVEC4-10 to produce angiogenic factors and nitric oxide and to activate VEGF-receptor pathways. This study also reports that Ag NP increase angiogenesis in Matrigel plug assay.…”

Section: Introductionmentioning

confidence: 93%

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Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells

Castiglioni¹

2014

WJBC

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croscope. RESULTS:Ag NP are cytotoxic in a dose and time dependent fashion for HMEC. At high concentrations, Ag NP determine loss of membrane integrity as demonstrated by the increased activity of lactate dehydrogenase in the culture medium. Ag NP rapidly stimulate the formation of free radicals. However, pre-incubation with Trolox, apocynin, or N-acetyl-L-cysteine, antioxidants which have different structure and act through different mechanisms, is not sufficient to prevent cytotoxicity. Ag NP also induce DNA damage dose-dependently, as shown by comet assay. When exposed to sublethal concentrations of Ag NP for long times, the cells remain viable but are growth retarded. Interestingly, removal of Ag NP partially rescues cell growth. Also genotoxicity is reversible upon removal of Ag NP from culture medium, suggesting that no permanent modifications occur. It is noteworthy that Ag NP are cytotoxic and genotoxic also for endothelial progenitors, in particular for endothelial colony-forming cells, which participate to angiogenesis. CONCLUSION:Silver nanoparticles are cytotoxic and genotoxic for human microvascular endothelial cells and might become a useful tool to control excessive angiogenesis.© 2014 Baishideng Publishing Group Inc. All rights reserved.Key words: Endothelial cells; Nanoparticles; Genotoxicity; Cytotoxicity; Angiogenesis Core tip: We studied the sensitivity to silver nanoparticles of microvascular endothelial cells, which are responsible for tissue homeostasis and fundamental in angiogenesis. Silver nanoparticles are cytotoxic and lead to membrane leakage. Cytotoxicity is not prevented by the antioxidants Trolox, N-acetyl-L-cysteine or apocynin. Silver nanoparticles also induce DNA damage as demonstrated by comet assay. When exposed to sublethal concentrations of silver nanoparticles for long times, the cells remain viable but are growth retarded. METHODS:We cultured human microvascular endothelial cells and endothelial colony-forming cells in their corresponding growth medium. Stock solutions of Ag NP were prepared in culture medium and sonicated before use. They were added at different concentrations and for different times to culture media. The toxicity of Ag NP was investigated by measuring the reduction of yellow tetrazolium salt to dark purple formazan (MTT assay) at 575 nm. After staining with trypan blue, cell proliferation was assessed by counting viable cells. The lactate dehydrogenase leakage assay was performed on culture media by following the oxidation of NADH to NAD+ and monitoring the reaction kinetically at 340 nm. Reactive oxygen species production was quantified using 2'-7'-dichlorofluorescein diacetate. The alkaline comet assay was performed after mixing the cells with low melting-point agarose. Electrophoresis was then conducted and the samples were stained with ethidium bromide and analyzed with a fluorescence mi-

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

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Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells

Castiglioni¹

2014

WJBC

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“…4 The nanoparticles showed potent antibacterial activity and a significantly higher synergistic effect with erythromycin, methicillin, and ciprofloxacin. 5 Silver nanoparticles are also reported to possess antifungal, 6 anti-inflammatory, 7 antiviral, 8 anti-angiogenesis, 9 and antiplatelet 10 activities. They all confer to them a major advantage for the development of alternative products against, for example, multidrug-resistant microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat. extract and their application in clinical ultrasound gel

He¹,

Du²,

Lv³

et al. 2013

IJN

138

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Eco-friendly green synthesis with plant extracts plays a very important role in nanotechnology, without any harmful chemicals. In this report, the synthesis of water-soluble silver nanoparticles was developed by treating silver ions with Chrysanthemum morifolium Ramat. extract at room temperature. The effect of the extract on the formation of silver nanoparticles was characterized by ultraviolet and visible absorption spectroscopy, X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The ultraviolet and visible absorption spectroscopy results show a strong resonance centered on the surface of silver nanoparticles (AgNP) at 430 nm. The Fourier transform infrared spectroscopy spectral study demonstrates Chrysanthemum morifolium Ramat. extract acted as the reducing and stabilizing agent during the synthesis. The X-ray diffraction analysis confirmed that the synthesized AgNP are single crystallines, corresponding with the result of transmission electron microscopy. Water-soluble AgNP, with an approximate size of 20 nm-50 nm were also observed in the transmission electron microscopy image. The bactericidal properties of the synthesized AgNP were investigated using the agar-dilution method and the growth-inhibition test. The results show the AgNP had potent bactericidal activity on Staphylococcus aureus and Escherichia coli, as well as a strong antibacterial activity against gram-negative bacteria, as compared to gram-positive bacteria with a dose-dependent effect, thus providing a clinical ultrasound gel with bactericidal property for prevention of cross infections.

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“…The antimicrobial properties of silver nanoparticles (AgNPs) have made these particles one of the most frequently used nanomaterials in consumer products (Kang et al, 2011). However, aggregation may reduce the specific area (surface area to volume ratio) of these tiny particles and thus weaken their antimicrobial effect (Liang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Organic matter induced mobilization of polymer-coated silver nanoparticles from water-saturated sand

Yang

Yin

Chen

et al. 2015

Science of The Total Environment

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Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles

Cited by 87 publications

References 36 publications

Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells

Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells

Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat. extract and their application in clinical ultrasound gel

Organic matter induced mobilization of polymer-coated silver nanoparticles from water-saturated sand

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