Vascular Tissue Banking: State of the Art

Buzzi, Marina; Mirelli, Michele; Vaselli, Cristiana; Tazzari, Pl; Terzi, A.; Stella, Andréa; Conte, Roberto

doi:10.1016/j.transproceed.2005.06.060

Cited by 11 publications

(8 citation statements)

References 4 publications

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“…5,56 Also, since cryopreservation is often performed in supplemented cell culture media, high concentrations of serum are often already present in the cryopreservation solution. 6,46,57 However, especially for the use in tissue banking, serum-free cryopreservation is desirable, as serum is i ) undefined and ii ) of animal origin.…”

Section: Discussionmentioning

confidence: 99%

“…For long-term storage, the only option is cryopreservation. The current gold standard used in vessel banking is cryopreservation in various serum-containing cell culture media (M 199, 3 RPMI 4,5 ) with addition of cryoprotective agents (mostly DMSO) and sometimes other additives like human albumin. 5 However, very modest results are achieved with most current freezing protocols in terms of muscular and especially endothelial function and integrity.…”

Section: Introductionmentioning

confidence: 99%

“…The current gold standard used in vessel banking is cryopreservation in various serum-containing cell culture media (M 199, 3 RPMI 4,5 ) with addition of cryoprotective agents (mostly DMSO) and sometimes other additives like human albumin. 5 However, very modest results are achieved with most current freezing protocols in terms of muscular and especially endothelial function and integrity. 6–9 In the clinical setting, an impaired endothelial lining induces platelet adhesion and clot formation, so it is highly desirable to preserve the endothelial layer of cryopreserved vessels for transplantation purposes.…”

Section: Introductionmentioning

confidence: 99%

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Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

2018

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Cryopreservation is the only long-term storage option for the storage of vessels and vascular constructs. However, endothelial barrier function is almost completely lost after cryopreservation in most established cryopreservation solutions. We here aimed to improve endothelial function after cryopreservation using the 2D-model of porcine aortic endothelial cell monolayers. The monolayers were cryopreserved in cell culture medium or cold storage solutions based on the 4°C vascular preservation solution TiProtec®, all supplemented with 10% DMSO, using different temperature gradients. After short-term storage at −80°C, monolayers were rapidly thawed and re-cultured in cell culture medium. Thawing after cryopreservation in cell culture medium caused both immediate and delayed cell death, resulting in 11 ± 5% living cells after 24 h of re-culture. After cryopreservation in TiProtec and chloride-poor modifications thereof, the proportion of adherent viable cells was markedly increased compared to cryopreservation in cell culture medium (TiProtec: 38 ± 11%, modified TiProtec solutions ≥ 50%). Using these solutions, cells cryopreserved in a sub-confluent state were able to proliferate during re-culture. Mitochondrial fragmentation was observed in all solutions, but was partially reversible after cryopreservation in TiProtec and almost completely reversible in modified solutions within 3 h of re-culture. The superior protection of TiProtec and its modifications was apparent at all temperature gradients; however, best results were achieved with a cooling rate of −1°C/min. In conclusion, the use of TiProtec or modifications thereof as base solution for cryopreservation greatly improved cryopreservation results for endothelial monolayers in terms of survival and of monolayer and mitochondrial integrity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

2018

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“…Thus, to our knowledge, this study is the first to report the use of arterial cryopreserved homografts for venous reconstruction of the PV/SMV after pancreatic resection. However, in the literature, it has widely been accepted that cryopreserved arterial homografts are safe and effective [20][21][22] .…”

Section: Discussionmentioning

confidence: 99%

Portal/Superior Mesenteric Vein Reconstruction during Pancreatic Resection Using a Cryopreserved Arterial Homograft

et al. 2015

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Background: Portal-superior mesenteric vein (PV/SMV) resection during pancreatic resection has been widely applied in clinical practice. Methods: From a prospective data base of pancreatic resections, patients undergoing PV/SMV resection and reconstruction with a cryopreserved arterial homograft were extracted with the aim of evaluating the safety, feasibility and reproducibility of the procedure. Data regarding patient demographics, preoperative staging, surgery, histopathology and postoperative outcomes were analyzed. Results: Five patients were extracted in the last year. Indications for this technique were type IV-V degree of vein involvement and a 3.5 cm median length of vein infiltration. Median operative and clamping times were satisfactory (385 and 27 min, respectively), postoperative outcomes were good and there was no graft infection, thrombosis or stenosis occurred postoperatively and during the follow-up period. Conclusion: The use of a cryopreserved arterial homograft for PV/SMV reconstruction after pancreatic resection seems to be a feasible, safe and easily reproducible surgical technique in high-volume specialized centers and can be added to the pool of surgical solutions in selected patients.

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“…Cryopreserved venous grafts can be used to reconstruct portal flow (4,5). We herein present three cases undergoing adult living donor liver transplantation in which reconstruction of portal flow was achieved with a cryopreserved vein graft from an engorged coronary vein to the graft portal vein.…”

mentioning

confidence: 99%

Application of cryopreserved vein grafts as a conduit between the coronary vein and liver graft to reconstruct portal flow in adult living liver transplantation

Wu¹,

Chou²,

Pan³

et al. 2009

Clinical Transplantation

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Adult-to-adult living donor liver transplantation is an alternative to donation from a deceased individual, and can help relieve the shortage of liver donations available for adult patients in Asian countries. When transplant candidates have thrombosis and deterioration of the portal vein, living donor liver transplantation is relatively contraindicated because portal veins in the grafts are short and vein grafts may not be available to reconstruct the portal vein. From June 2003 to May 2007, 82 adult living donor liver transplantations were performed at Chang-Gung Memorial Hospital. Three patients had portal vein thrombosis and marked fibrosis of the portal vein and cryopreserved vein grafts were used to reconstruct portal flow from the engorged coronary vein to the graft portal vein. All vein grafts are patent and all patients have normal liver function at 21-36 months after transplantation. When cryopreserved vein grafts are available, adult living donor liver transplantation can be successfully performed in patients with marked deterioration of the portal vein. The short distance from the engorged coronary vein to the graft portal vein may decrease the incidence of re-thrombosis of the venous conduit.

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Vascular Tissue Banking: State of the Art

Cited by 11 publications

References 4 publications

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

Portal/Superior Mesenteric Vein Reconstruction during Pancreatic Resection Using a Cryopreserved Arterial Homograft

Application of cryopreserved vein grafts as a conduit between the coronary vein and liver graft to reconstruct portal flow in adult living liver transplantation

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