Vascular-Targeted Photothermal Therapy of An Orthotopic Murine Glioma Model

Abstract

Aim To develop nanoshells for vascular-targeted photothermal therapy of glioma. Materials & methods The ability of nanoshells conjugated to VEGF and/or poly(ethylene glycol) (PEG) to thermally ablate VEGF receptor-2 positive endothelial cells upon near-infrared laser irradiation was evaluated in vitro. Subsequent in vivo studies evaluated therapy in mice bearing intracerebral glioma tumors by exposing tumors to near-infrared light after systemically delivering saline, PEG-coated nanoshells, or VEGF-coated nanoshells. The treatment effect was monitored with intravital microscopy and histology. Results VEGF-coated but not PEG-coated nanoshells bound VEGF receptor-2 positive cells in vitro to enable targeted photothermal ablation. In vivo, VEGF targeting doubled the proportion of nanoshells bound to tumor vessels and vasculature was disrupted following laser exposure. Vessels were not disrupted in mice that received saline. The normal brain was unharmed in all treatment and control mice. Conclusion Nanoshell therapy can induce vascular disruption in glioma.

Nanoshell-mediated photothermal therapy can enhance chemotherapy in inflammatory breast cancer cells

“…7,8 Thus, PTT is advantageous for cancer treatment because it is highly effective, minimally invasive, and offers limited side effects. Accordingly, there are several gold-based, NIRabsorbing nanoparticles being investigated for PTT, including silica core-gold shell nanoshells, 5,9,10 gold nanorods, 11 gold-gold sulfide nanoparticles, 12 hollow gold nanospheres, 13 and gold nanocages. 14 We selected nanoshells for use in our study, which demonstrates that low-dose PTT can sensitize cancer cells to chemotherapy by promoting drug accumulation in cells, because they are the furthest along in clinical development and are currently being evaluated in multiple clinical trials.…”

“…9,33 To evaluate the combined effects of PTT and dox, subtherapeutic doses of each treatment were administered either alone or in combination. By using a dose of dox that causes minimal to no cell death, enhancement of chemotherapy via PTT could be assessed.…”

Noble Metal Nanoparticles in Cancer Therapy: Properties Challenges and Clinical Applications.

“…Gold and silica nanoparticles containing endothelial growth factor [VEGF]-targeted nanoshellꞌs for thermal ablation and vessel disruption in mouse glioma model and it is in clinical tr ials for head and neck cancer and primary and/or metastatic lung tumors. (34) Current research field present a variety of nanoparticles, and each has its own unique properties and applications suc h as nanorings, nanoshells, nanorods, nanopores and nanowires, etc.…”

Nanomedicines for Cancer Therapy: An Update of FDA Approved and Those under Various Stages of Development

“…This is referred to as AuroLase therapy. [84][85][86][87][88][89] The potential benefit of AuroLase therapy is the highly selective and rapid tumor destruction with minimal damage to surrounding healthy tissue. Preclinical studies have demonstrated that AuroLase Therapy is effective and causes no detectable systemic toxicity.…”