Vascular Pseudoinvasion in Laparoscopic Hysterectomy Specimens for Endometrial Carcinoma

Abstract

Over several years, it has been a matter of debate whether or not the use of a uterine balloon manipulator during laparoscopic hysterectomies for endometrial carcinoma (EC) may cause tumor disruption resulting in a positive peritoneal cytology. More recently, this procedure has been associated with vascular pseudoinvasion in cases of low-risk EC. In this study, we evaluated a series of 21 cases of low-risk EC treated by laparoscopic hysterectomy (LH) to determine the incidence of this finding and to better characterize its histopathologic features. In addition, we reviewed 28 cases of low-risk EC treated by total abdominal hysterectomy (TAH) for comparison. Clinical information was obtained from patients' charts. Hematoxylin and eosin-stained slides were retrospectively reviewed in all cases. The following information was recorded: tumor grade and tumor stage according to the International Federation of Gynecology and Obstetrics, tumor shape (polypoid versus flat), presence or absence of vascular space involvement (VSI), size and location of the vessels with tumor involvement, concomitant presence of artifactual clefts in the myometrium with tumor involvement if applicable, presence or absence of lymph node sampling and the presence or absence of involvement at this site, and results of peritoneal cytology. Seven of 21 (33%) cases of low-risk EC treated by LH in this study showed VSI. None of the cases treated by TAH had VSI (P=0.001). In all of the cases of LH with VSI, the endometrial tumor was polypoid. VSI was detected only in large, thick-walled vessels in the outer myometrium or in ectatic vessels anywhere in the myometrium; no tumor fragments were seen in small vessels. The tumor in the VSI consisted of conspicuous fragments of tumors detached from the vascular wall. The VSI also lacked the inflammatory perivascular infiltrate seen in many cases of bona fide lymphovascular invasion. In addition, all of the cases with VSI also showed fragments of tumor in artifactual clefts in the myometrium. None of the cases of LH in which lymph node sampling and/or peritoneal cytology were obtained showed tumor at this site. In summary, our study confirms that LH is indeed associated with a higher rate of vascular pseudoinvasion when compared with TAH. However, we cannot attribute this phenomenon to mechanical disruption, displacement, and transport of tumor tissue into vascular spaces by the use of a uterine manipulator alone. Instead, we propose that pathologists may be generating postoperative pseudoinvasion by mechanically transporting tumor into vascular spaces during the grossing process. Proper recognition of this artifact is of utmost importance to avoid the overtreatment of patients undergoing LH for low-risk EC.

Evaluation of vascular space involvement in endometrial adenocarcinomas: laparoscopic vs abdominal hysterectomies

“…In contrast to the two previously published studies, which addressed vascular space involvement by endometrial carcinoma in total laparoscopic hysterectomies, 7,8 this study did not find a significant difference in the rate of vascular space involvement between total laparoscopic hysterectomy and total abdominal hysterectomy. Nevertheless, there was a trend towards a higher frequency of vascular space involvement in the total laparoscopic hysterectomy group and the lack of a strong correlation can be related to a number of issues.…”

“…8 In the latter they noted that the tumor cells were mostly in large vessels in the outer aspect of the myometrium but not in small vascular spaces. Sometimes, the tumor cells were associated with stroma, and the vascular space involvement was only seen in cases with exophytic tumors.…”

Efficacy and safety of uterine manipulators in laparoscopic surgery: a review

“…In both cervical and endometrial malignancies, clinico-pathological parameters such as infiltration depth and lymphovascular space invasion (LVSI) may be influenced when a manipulator is used [27][28][29][30][31]. However, it is hypothesised that other factors such as artefacts and tissue handling contributed to these findings.…”

Recent Developments in Surgical Pathology of the Uterine Corpus

“…88 Others propose that LVPI is a grossing artifact, arguing that displacement of endometrial glands and stroma is a result of manipulation of the specimen in the gross room and is more likely to occur in exophytic tumors. 87,89 However, it should also be noted that there can be significant interobserver variability in assessing lymphovascular space invasion. Most importantly, vascular invasion is known to be an independent prognostic factor in endometrial carcinoma.…”