Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices

Cho, Seung Woo; Park, Hee Jung; Ryu, Jee Hoon; Kim, Soo Hyun; Kim, Young Ha; Choi, Cha Yong; Lee, Minjae; Kim, Jong Sung; Jang, In Sung; Kim, Dong Ik; Kim, Byung Soo

doi:10.1016/j.biomaterials.2004.06.018

Cited by 85 publications

(67 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, currently used devices lack regeneration and growth potential, leading to subsequent reoperations with high morbidity and mortality. [2][3][4] Therefore, the development of biocompatible materials whose growth would keep pace with that of the child [5][6][7][8][9][10][11] still remains a clinically relevant issue.…”

Section: Introductionmentioning

confidence: 99%

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Pontailler

Illangakoon

Williams

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

As part of a program targeted at developing a resorbable valved tube for replacement of the right ventricular outflow tract, we compared three biopolymers (polyurethane [PU], polyhydroxyalkanoate (the poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxyvalerate) [PHBVV]), and polydioxanone [PDO]) and two biofunctionalization techniques (using adipose-derived stem cells [ADSCs] or the arginine-glycine-aspartate [RGD] peptide) in a rat model of partial inferior vena cava (IVC) replacement. Fifty-three Wistar rats first underwent partial replacement of the IVC with an acellular electrospun PDO, PU, or PHBVV patch, and 31 nude rats subsequently underwent the same procedure using a PDO patch biofunctionalized either by ADSC or RGD. Results were assessed both in vitro (proliferation and survival of ADSC seeded onto the different materials) and in vivo by magnetic resonance imaging ( MRI), histology, immunohistochemistry [against markers of vascular cells (von Willebrand factor [vWF], smooth muscle actin [SMA]), and macrophages ([ED1 and ED2] immunostaining)], and enzyme-linked immunosorbent assay (ELISA; for the expression of various cytokines and inducible NO synthase). PDO showed the best in vitro properties. Six weeks after implantation, MRI did not detect significant luminal changes in any group. All biopolymers were evenly lined by vWF-positive cells, but only PDO and PHBVV showed a continuous layer of SMA-positive cells at 3 months. PU patches resulted in a marked granulomatous inflammatory reaction. The ADSC and RGD biofunctionalization yielded similar outcomes. These data confirm the good biocompatibility of PDO and support the concept that appropriately peptide-functionalized polymers may be successfully substituted for cell-loaded materials.

show abstract

Section: Introductionmentioning

confidence: 99%

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Pontailler

Illangakoon

Williams

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Recently, it has been reported that BMCs are capable of differentiating into ECs [35][36][37] and vascular SMCs. [38][39][40] Indeed, BMCs have been shown as a feasible cell source for the engineering of cardiovascular tissues in animals 7,9,[16][17][18] and humans. 41,42 In addition, the use of BMCs as a cell source for vascular graft engineering is considered less invasive than harvesting vascular cells from autologous blood vessels.…”

Section: Discussionmentioning

confidence: 99%

“…[16][17][18] In brief, bone marrow (30 mL per dog) was aspirated from the humeri of healthy mongrel dogs (12 months old, 20-25 kg, n 5 7) with a syringe containing heparin (100 units heparin/mL bone marrow). Aspirated bone marrow was mixed with an equal volume of phosphate-buffered saline (PBS, Sigma, St. Louis, MO) solution and centrifuged on Ficoll-Paque density gradient (Amersham Biosciences, Arlington Heights, IL) for 30 min at 2000 rpm.…”

Section: Bmc Isolation and Culturementioning

confidence: 99%

Tissue‐Engineered Blood Vessels With Endothelial Nitric Oxide Synthase Activity

et al. 2008

Self Cite

View full text Add to dashboard Cite

Nondegradable synthetic polymer vascular grafts used in cardiovascular surgery have shown serious shortcomings, including thrombosis, calcification, infection, and lack of growth potential. Tissue engineering of vascular grafts with autologous stem cells and biodegradable polymeric materials could solve these problems. The present study is aimed to develop a tissue-engineered vascular graft (TEVG) with functional endothelium using autologous bone marrow-derived cells (BMCs) and a hybrid biodegradable polymer scaffold. Hybrid biodegradable polymer scaffolds were fabricated from poly(lactide-co-e-caprolactone) (PLCL) copolymer reinforced with poly(glycolic acid) (PGA) fibers. Canine bone marrow mononuclear cells were induced in vitro to differentiate into vascular smooth muscle cells and endothelial cells. TEVGs (internal diameter: 10 mm, length: 40 mm) were fabricated by seeding vascular cells differentiated from BMCs onto PGA/PLCL scaffolds and implanted into the abdominal aorta of bone marrow donor dogs (n 5 7). Eight weeks after implantation of the TEVGs, the vascular grafts remained patent. Histological and immunohistochemical analyses of the vascular grafts retrieved at 8 weeks revealed the regeneration of endothelium and smooth muscle and the presence of collagen. Western blot analysis showed that endothelial nitric oxide synthase (eNOS) was expressed in TEVGs comparable to native abdominal aortas. This study demonstrates that vascular grafts with significant eNOS activity can be tissueengineered with autologous BMCs and hybrid biodegradable polymer scaffolds. '

show abstract

“…Decellularized blood vessels provide a scaffold with the complex structure of the native tissue and are highly supportive of EC adhesion. [84][85][86][87] Ovine EOCs seeded onto decellularized porcine iliac arteries were pre-conditioned with a fluid shear stress of 25 dyn/cm 2 and produced NO upon stimulation with a thromboxane analog. 27 When implanted into sheep, the EOC-seeded grafts remained patent for approximately 130 days.…”

Section: Naturally Derived and Biodegradable Scaffoldingmentioning

confidence: 99%

Endothelial Outgrowth Cells: Function and Performance in Vascular Grafts

Glynn

Hinds

2014

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

The clinical need for vascular grafts continues to grow. Tissue engineering strategies have been employed to develop vascular grafts for patients lacking sufficient autologous vessels for grafting. Restoring a functional endothelium on the graft lumen has been shown to greatly improve the long-term patency of small-diameter grafts. However, obtaining an autologous source of endothelial cells for in vitro endothelialization is invasive and often not a viable option. Endothelial outgrowth cells (EOCs), derived from circulating progenitor cells in peripheral blood, provide an alternative cell source for engineering an autologous endothelium. This review aims at highlighting the role of EOCs in the regulation of processes that are central to vascular graft performance. To characterize EOC performance in vascular grafts, this review identifies the characteristics of EOCs, defines functional performance criteria for EOCs in vascular grafts, and summarizes the existing work in developing vascular grafts with EOCs.

show abstract

Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices

Cited by 85 publications

References 31 publications

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Tissue‐Engineered Blood Vessels With Endothelial Nitric Oxide Synthase Activity

Endothelial Outgrowth Cells: Function and Performance in Vascular Grafts

Contact Info

Product

Resources

About