Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis

Costa, Carolina S.; Bravo, Juliana Pereira; Ribeiro, Cintia; Soprano, Adriana Santos; Sassaki, Flávio Tetsuo; Maia, Ivan de Godoy

doi:10.1007/s11240-017-1276-6

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…Thus far, the role of AKT1 and AtHAK5 in Rb + translocation within vascular cells cannot be discarded. Expression of AKT1‐like and HAK5‐like genes in vascular tissues has been established before (Chen et al, ; Costa et al, ; Li et al, ; Yang et al, ). Thus, AKT1 and AtHAK5 expression in root tissues was verified with promoter::GUS fusions (Figure ).…”

Section: Resultsmentioning

confidence: 98%

Modulation of K⁺translocation by AKT1 and AtHAK5 in Arabidopsis plants

Nieves‐Cordones

Lara

Ródenas

et al. 2019

Plant Cell & Environment

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Root cells take up K + from the soil solution, and a fraction of the absorbed K + is translocated to the shoot after being loaded into xylem vessels. K + uptake and translocation are spatially separated processes. K + uptake occurs in the cortex and epidermis whereas K + translocation starts at the stele. Both uptake and translocation processes are expected to be linked, but the connection between them is not well characterized. Here, we studied K + uptake and translocation using Rb + as a tracer in wild-type Arabidopsis thaliana and in T-DNA insertion mutants in the K + uptake or translocation systems. The relative amount of translocated Rb + to the shoot was positively correlated with net Rb + uptake rates, and the akt1 athak5 T-DNA mutant plants were more efficient in their allocation of Rb + to shoots. Moreover, a mutation of SKOR and a reduced plant transpiration prevented the full upregulation of AtHAK5 gene expression and Rb + uptake in K + -starved plants. Lastly, Rb + was found to be retrieved from root xylem vessels, with AKT1 playing a significant role in K + -sufficient plants. Overall, our results suggest that K + uptake and translocation are tightly coordinated via signals that regulate the expression of K + transport systems.

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Section: Resultsmentioning

confidence: 98%

Modulation of K⁺translocation by AKT1 and AtHAK5 in Arabidopsis plants

Nieves‐Cordones

Lara

Ródenas

et al. 2019

Plant Cell & Environment

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show abstract

“…Most HAK genes in plants are expressed mainly in the root system with tissue speci city, while a few members are expressed in multiple plant tissues (Bañuelos et al, 2002). However, recent studies have found that the EgHAK5 promoter in Eucalyptus derives GUS expression not only in the Arabidopsis root system but also in the leaf vein and embryo axis vessel tissue (Costa et al, 2017). Present study found that the GUS driven by pScHAK10 was expressed in the root system and leaves of A. thaliana.…”

Section: Discussionmentioning

confidence: 55%

“…For example, the potassium transporter HAK5 is mainly expressed in the root systems. The promoters of the AtHAK5 gene in Arabidopsis and OsHAK5 gene in rice drive the GUS gene to express in the root system, which tissue speci city but also can be induced by low potassium conditions to stimulate gene expression (Santa-María et al, 1997; Ahn et al, 2004;Costa et al, 2017). Most HAK genes in plants are expressed mainly in the root system with tissue speci city, while a few members are expressed in multiple plant tissues (Bañuelos et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional analysis of ScHAK10 gene promoter from sugarcane (Saccharum officinarum L.)

Luo,

Huang,

Cao

et al. 2024

Preprint

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Transcriptional regulation of the high-affinity K+ (HAK) transporter gene is an important mechanism of plant response to low potassium stress. Cloning and analysis of the promoter of potassium transporter gene is not only helpful to its expression pattern and regulation mechanism but also important to improve the potassium absorption efficiency in sugarcane. The potassium transporter gene ScHAK10 is highly expressed and induced by low potassium stress in sugarcane, but the functions of its promoter is still unclear. In the present article, the 1918 bp promoter region of the ScHAK10 gene (pScHAK10) was cloned by genomic walking technique. Computational analysis affirmed the existence of abiotic stress-responsive cis and core cis-elements, such as TATA box, CAAT box, phytohormone responsive, stress response and light response motifs. GUS histochemical staining of transgenic Arabidopsis thaliana seedlings showed that the leaf, corolla, and root tip were deeply stained, and paraffin sections of root tip tissue showed GUS staining in ductal tissue of A. thaliana shallowly stained. The 5′-terminal deletion of the promoter was cloned, and the lengths of 1918 (full), 1623 (Q1), 1332 (Q2), 957 (Q3), 576 (Q4), and 357 bp (Q5) were cloned into the GUS reporter vector for A. thaliana transient transformation. The transgenic plants generated through a single event exhibited a promising expression of the GUS reporter protein, which was treated with salt, low potassium, IAA and cold stress conditions. The results showed that the promoter activity correlates with the promoter fragment's length, and the long promoter fragment exhibits higher training. The Q5 was the least active and could not drive GUS expression. Under abiotic stress, the expression of GUS enzyme activity varies among different promoter fragments. Under low potassium and high salt stress, Q3 and Q4 showed the highest promoter activity. The Q1 and Q4 led the highest promoter activity during IAA and cold stress. These findings help to understand the molecular mechanism of ScHAK10 expression regulation and provide a theoretical basis for improving potassium uptake efficiency and breeding potassium-efficient varieties in sugarcane.

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“…For example, the potassium transporter HAK5 is mainly expressed in the root systems. The promoters of the AtHAK5 gene in Arabidopsis and OsHAK5 gene in rice drive the GUS gene to express in the root system, which tissue speci city but also can be induced by low potassium conditions to stimulate gene expression [13][14][15]. Most HAK genes in plants are expressed mainly in the root system with tissue speci city, while a few members are expressed in multiple plant tissues [16].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional analysis of ScHAK10 gene promoter from sugarcane (Saccharum officinarum L.)

Luo,

Huang,

Cao

et al. 2024

Preprint

View full text Add to dashboard Cite

Transcriptional regulation of the high-affinity K+ (HAK) transporter gene is an important mechanism of plant response to low potassium stress. Cloning and analysis of the promoter of potassium transporter gene is not only helpful to its expression pattern and regulation mechanism but also important to improve the potassium absorption efficiency in sugarcane. The potassium transporter gene ScHAK10 is highly expressed and induced by low potassium stress in sugarcane，but the functions of its promoter is still unclear. In the present article, the 1918 bp promoter region of the ScHAK10 gene (pScHAK10) was cloned by genomic walking technique. Computational analysis affirmed the existence of abiotic stress-responsive cis and core cis-elements, such as TATA box, CAAT box, phytohormone responsive, stress response and light response motifs. GUS histochemical staining of transgenic Arabidopsis thaliana seedlings showed that the leaf, corolla, and root tip were deeply stained, and paraffin sections of root tip tissue showed GUS staining in ductal tissue of A. thaliana shallowly stained. The 5′-terminal deletion of the promoter was cloned, and the lengths of 1918 (full), 1623 (Q1), 1332 (Q2), 957 (Q3), 576 (Q4), and 357 bp (Q5) were cloned into the GUS reporter vector for A. thaliana transient transformation. The transgenic plants generated through a single event exhibited a promising expression of the GUS reporter protein, which was treated with salt, low potassium, IAA and cold stress conditions. The results showed that the promoter activity correlates with the promoter fragment's length, and the long promoter fragment exhibits higher training. The Q5 was the least active and could not drive GUS expression. Under abiotic stress, the expression of GUS enzyme activity varies among different promoter fragments. Under low potassium and high salt stress, Q3 and Q4 showed the highest promoter activity. The Q1 and Q4 led the highest promoter activity during IAA and cold stress. These findings help to understand the molecular mechanism of ScHAK10 expression regulation and provide a theoretical basis for improving potassium uptake efficiency and breeding potassium-efficient varieties in sugarcane.

show abstract

Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis

Cited by 9 publications

References 34 publications

Modulation of K⁺translocation by AKT1 and AtHAK5 in Arabidopsis plants

Modulation of K⁺translocation by AKT1 and AtHAK5 in Arabidopsis plants

Molecular cloning and functional analysis of ScHAK10 gene promoter from sugarcane (Saccharum officinarum L.)

Molecular cloning and functional analysis of ScHAK10 gene promoter from sugarcane (Saccharum officinarum L.)

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Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis

Cited by 9 publications

References 34 publications

Modulation of K+translocation by AKT1 and AtHAK5 in Arabidopsis plants

Modulation of K+translocation by AKT1 and AtHAK5 in Arabidopsis plants

Molecular cloning and functional analysis of ScHAK10 gene promoter from sugarcane (Saccharum officinarum L.)

Molecular cloning and functional analysis of ScHAK10 gene promoter from sugarcane (Saccharum officinarum L.)

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Modulation of K⁺translocation by AKT1 and AtHAK5 in Arabidopsis plants

Modulation of K⁺translocation by AKT1 and AtHAK5 in Arabidopsis plants