Vascular endothelial growth factors and their receptors in the novel human cell line, HN-Eso-1, established from esophageal spindle cell carcinoma

Nakatani, Hajime; Akimori, Toyokazu; Takezaki, Yuka; Hanazaki, Kazuhiro

doi:10.2152/jmi.57.232

Cited by 3 publications

(3 citation statements)

References 7 publications

(3 reference statements)

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“…VEGF-C is regarded as a crucial factor for regulating lymphogenesis, acting via VEGFR-3. Certain studies have also demonstrated that there is a correlation between tumor VEGF-C expression and lymph node metastasis (41)(42)(43). This suggested that blocking the expression of VEGF-C in the tumor cells can relieve lymphatic invasion and lymph node metastasis.…”

Section: Discussionmentioning

confidence: 98%

Inhibiting the proliferation and metastasis of hilar cholangiocarcinoma cells by blocking the expression of vascular endothelial growth factor with small interfering RNA

Ouyang

Tang

2018

Oncol Lett

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The aim of the present study was to investigate whether the proliferation and metastasis of hilar cholangiocarcinoma cells can be suppressed and whether apoptosis can be induced by small interfering RNA (siRNA) repression of vascular endothelial growth factor (VEGF). siRNA sequences targeting the gene were designed and the human hilar cholangiocarcinoma QBC939, HCCC-9810 and RBE cell lines were transfected with VEGF-siRNA plasmids for 48 h. Reverse transcription-quantitative polymerase chain reaction and western blotting measured the levels of VEGF-A, VEGF-C and matrix metalloproteinase 2 (MMP2) mRNA expression and protein content. The cell invasion potential was evaluated using the Transwell invasion and migration assay and the MTT assay was employed to detect the proliferation of hilar cholangiocarcinoma cells. Flow cytometry was used to quantify cell apoptosis and necrosis. Following the transfection of VEGF-siRNA, a significant reduction of mRNA and protein levels of VEGF-A, VEGF-C and MMP2 was observed in the hilar cholangiocarcinoma cells. The invasion, migration and proliferation of tumor cells were also notably decreased. The rate of tumor cell apoptosis was increased in the VEGF-siRNA group (15.42%) compared with the non-siRNA control (2.22%) and the negative control (2.71%) groups. It was concluded that blocking the expression of VEGF via VEGF-siRNA effectively inhibited the invasion, migration and proliferation, and induced apoptosis in hilar cholangiocarcinoma cells. These observations suggested that targeting VEGF with RNAi may be an effective therapeutic strategy for treating hilar cholangiocarcinoma.

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Section: Discussionmentioning

confidence: 98%

Inhibiting the proliferation and metastasis of hilar cholangiocarcinoma cells by blocking the expression of vascular endothelial growth factor with small interfering RNA

Ouyang

Tang

2018

Oncol Lett

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“…It could effectively inhibit VEGF-stimulated endothelial cell migration as well as proliferation and decrease tumor micro vessel density [15]. In addition, Apatinib could prevent multidrug resistance conferred by ABCB1 and ABCG2 proteins [16].…”

Section: Discussionmentioning

confidence: 99%

Response to apatinib in chemotherapy-failed advanced spindle cell breast carcinoma

et al. 2016

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Spindle cell carcinoma of the breast is a rare subtype of metaplastic carcinoma, and no effective chemotherapy special for metaplastic carcinoma exists until now. As spindle cell carcinomas of the breast are typically “Triple Negative”, endocrine therapy and molecular therapy targeted to Her2 might not be favorable, resulting in poor prognosis. Apatinib is currently being tested in patients with breast or lung cancers. Here we report a successful case using Apatinib to treat spindle cell carcinoma of breast.A 52- year- old woman presented with a gradually enlarged lump in left breast, which was revealed to be a triple-negative spindle cell carcinoma, underwent a modified radical mastectomy. After the first line chemotherapy with Cyclophosphamide and Epirubicin, multiple metastases in bilateral lung and left anterior thoracic wall appeared. After disease progressed with therapy of Bevacizumab combined with Albumin-bound Paclitaxel and Cisplatin, we treated the patient with Apatinib according to her VEGFR expression, which showed nearly complete response and controllable and tolerated side effects. Next-generation sequencing analysis of the tumor specimen and real time ctDNA was performed to observe the mutated gene numbers matched with therapeutic effect. The present case can help to provide a new and effective therapy strategy to treat advanced spindle cell carcinoma.

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“…qPCR analysis has previously revealed that VEGFA is upregulated in the HN-Eso-1 metastatic esophageal spindle cell carcinoma-derived cell line. VEGFA expression was reported to be involved in the HN-Eso-1 cell autocrine system and to have a protective effect against anti-tumor agents (41). HBEGF is a member of the epidermal growth factor family.…”

Section: Discussionmentioning

confidence: 99%

Pilot genome-wide study of tandem 3′ UTRs in esophageal cancer using high-throughput sequencing

Sun

Zhu

2014

Molecular Medicine Reports

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Regulatory regions within the 3′ untranslated region (UTR) influence polyadenylation (polyA), translation efficiency, localization and stability of mRNA. Alternative polyA (APA) has been considered to have a key role in gene regulation since 2008. Esophageal carcinoma is the eighth most common type of cancer worldwide. The association between polyA and disease highlights the requirement for comprehensive characterization of genome-wide polyA profiles. In the present study, global polyA profiles were established using the sequencing APA sites (SAPAS) method in order to elucidate the interrelation between 3′ UTR length and the development of esophageal cancer. PolyA profiles were analyzed in squamous cell carcinoma, with ~903 genes identified to have shortened 3′ UTRs and 917 genes identified to use distal polyA sites. The genes with shortened 3′ UTRs were primarily associated with adherens junctions and the cell cycle. Four differentially expressed genes were also found, among which three genes were observed to be upregulated in cancerous tissue and involved in the positive regulation of cell motion, migration and locomotion. One gene was found to be downregulated in cancerous tissue, and associated with oxidative phosphorylation. These findings suggest that esophagitis may have a key role in the development of esophageal carcinoma. Furthermore, the genes with tandem 3′ UTRs and differential expression identified in the present study may have the potential to be used as biomarkers for the diagnosis and prognosis of esophageal cancer.

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Vascular endothelial growth factors and their receptors in the novel human cell line, HN-Eso-1, established from esophageal spindle cell carcinoma

Cited by 3 publications

References 7 publications

Inhibiting the proliferation and metastasis of hilar cholangiocarcinoma cells by blocking the expression of vascular endothelial growth factor with small interfering RNA

Inhibiting the proliferation and metastasis of hilar cholangiocarcinoma cells by blocking the expression of vascular endothelial growth factor with small interfering RNA

Response to apatinib in chemotherapy-failed advanced spindle cell breast carcinoma

Pilot genome-wide study of tandem 3′ UTRs in esophageal cancer using high-throughput sequencing

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