Vascular Endothelial Growth Factor Receptor–2 Expression in the Pulp of Human Primary and Young Permanent Teeth

Mattuella, Letícia Grando; Figueiredo, José Antônio Poli de; Araújo, Fernando Borba de; Fossati, Anna Christina Medeiros

doi:10.1016/j.joen.2007.08.019

Cited by 31 publications

(24 citation statements)

References 38 publications

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“…VEGF expression in pulp fibroblasts and odontoblasts of human teeth is higher in immature than mature permanent teeth, suggesting a role of VEGF in tooth maturation (Q. . VEGFR-2 is expressed in endothelial cells of the tooth pulp of both primary and permanent human teeth and may function to respond to morphogenetic and survival signals mediated by VEGF (Grando Mattuella et al 2007). As stated previously, VEGF, as well as FGF, PDGF, and EGF, are found in dentin matrix (Roberts-Clark and Smith 2000).…”

Section: Discussionmentioning

confidence: 99%

Incisor Degeneration in Rats Induced by Vascular Endothelial Growth Factor/Fibroblast Growth Factor Receptor Tyrosine Kinase Inhibition

et al. 2010

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BMS-645737, an inhibitor of vascular endothelial growth factor (VEGF) receptor-2 and fibroblast growth factor (FGF) receptor-1, has antiangiogenic activity and was evaluated in nonclinical studies as a treatment for cancer. This article characterizes the BMS-645737-induced clinical, gross, and histologic lesions of incisor teeth in Sprague-Dawley (SD) rats. Rats received 0 800 mg/kg BMS-645737 in a single-dose study or consecutive daily doses of 0 20 mg/kg/day in a 1-month study. The reversibility of these effects was assessed in the 1-month study. White discoloration and fracture of incisors were observed clinically and grossly in the 1-month study. In both studies, dose-dependent histopathologic lesions of incisors were degeneration and/or necrosis of odontoblasts and ameloblasts; decreased mineralization of dentin; inflammation and necrosis of the dental pulp; and edema, congestion, and hemorrhage in the pulp and periodontal tissue adjacent to the enamel organ. Partial recovery was observed at lower doses after a two-week dose-free period in the one-month study. Drug-induced incisor lesions were considered to be related to the pharmacologic inhibitory effects on VEGF and FGF signaling, that is, inhibition of growth and maintenance of small-diameter vessels that support the formation of dentin and enamel in growing teeth and/or to perturbances of function of odontoblasts and ameloblasts or their precursors.

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Section: Discussionmentioning

confidence: 99%

Incisor Degeneration in Rats Induced by Vascular Endothelial Growth Factor/Fibroblast Growth Factor Receptor Tyrosine Kinase Inhibition

et al. 2010

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“…This process involves various angiogenic growth factors such as Vascular Endothelial Growth Factor (VEGF) and its family (VEGF-A, -B, -C, -D, -E), and platelet-derived growth factor (PDGF), which play important roles in the differentiation, growth and migration of endothelial cells [1]. Healthy and inflamed dental pulp contains VEGF, which plays a role in vascularization and may also trigger pulpitis [2].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia-mimicking bioactive glass regenerative effects on dental stem cells

Noor

Azevedo

Mohamad

et al. 2016

AIP Conference Proceedings

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Abstract.Vascularization is an important aspect of tissue regeneration. Hypoxia, low oxygen concentration, is a known stimulus for the release of vascular endothelial growth factors (VEGF) which play important roles in vascularization. The current study aimed to assess the effect of a cobalt-containing bioactive glass (BG) in stimulating hypoxia and promoting vascularization. To incorporate cobalt into BG, 1 mol% of calcium was substituting with cobalt, and this formulation was compared to the one without cobalt. Both BGs were processed via melt-derived method. The BG powders with particle size less than 38 μm were incubated with cell culture medium for 4 hours at 37°C on continuous rolling, and then the medium was filtered using 0.22 μm syringe filters. Prior to use, the BG-conditioned media were supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) antibiotic-antimycotic, and were allowed to equilibrate overnight inside a CO2 incubator. The conditioned media were used on human dental stem cells (stem cells from permanent (DPSC) and deciduous (SHED) teeth) and assessed for their capacity to stimulate the release of angiogenic factors from the cells. The results showed that cobalt ions were released from the cobalt-containing BG, following partial dissolution of the glasses in cell culture medium, and promoted VEGF release from the cells. In conclusion, the incorporation of cobalt in BG may have potential to be used for tissue regeneration by promoting vascularization through the activation of hypoxia pathway and the release of VEGF.

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“…VEGF acts as a paracrine factor whose signals are mediated by two type III tyrosine kinase receptors on the surface of endothelial cells: VEGFR-1 and VEGFR-2. VEGFR-2 is a major mediator of the mitogenic and angiogenic effects of VEGF (Grando et al, 2007). The platelet-endothelial cell adhesion molecule (CD31) is also an important marker of vascular endothelial cells in periodontal tissue (Gemmell et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Analysis of vascular distribution and growth factors in human gingival tissue associated with periodontal probing depth

Noguchi

Miwa

Sunohara

et al. 2011

Okajimas Folia Anat. Jpn.

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Summary: Vascular endothelial growth factor (VEgF) is a key regulator of blood vessel endothelium. tissue levels of this angiogenesis marker are unknown in human gingival tissue, as is the correlation between vascular growth factors and hypoxiainducible factor. we examined the expression of VEgF, type III tyrosine kinase receptors (VEgF-r2), platelet-endothelial cell adhesion molecule (cD31) and hypoxia-inducible factor (hIF) mrNA from human gingival tissue of the oral cavity. tissue samples were from a small quantity of gingival sample biopsy with gingival sulcular depth (gSD) < 2 mm (group 1), 2 to 4 mm (group 2), and > 4 mm (group 3). we found that the levels of VEgF-r2, cD31 and hIF mrNA were higher in the gingival tissue of group 2 than that of group 1, and VEgF in the group 3 was also higher than that of group 1. the different mrNA levels of these markers may reflect the mrNA levels reflect the vasculature state of gingival tissue based on gSD. VEgF-r2 and hIF also indicate the presence of an elongated blood vessel in the gingival tissue. In the early stage of angiogenesis, VEgF-r2 leads to expression of VEgF, and hIF-1 mediates increased VEgF expression in response to hypoxia in swollen tissues or during the expansion of periodontal tissues, which is useful in the early diagnosis of periodontal diseases.

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Vascular Endothelial Growth Factor Receptor–2 Expression in the Pulp of Human Primary and Young Permanent Teeth

Cited by 31 publications

References 38 publications

Incisor Degeneration in Rats Induced by Vascular Endothelial Growth Factor/Fibroblast Growth Factor Receptor Tyrosine Kinase Inhibition

Incisor Degeneration in Rats Induced by Vascular Endothelial Growth Factor/Fibroblast Growth Factor Receptor Tyrosine Kinase Inhibition

Hypoxia-mimicking bioactive glass regenerative effects on dental stem cells

Analysis of vascular distribution and growth factors in human gingival tissue associated with periodontal probing depth

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