Vascular endothelial growth factor levels are increased and associated with disease activity in patients with Behçet's syndrome

Çekmen, Mustafa; Evereklioğlu, Cem; Er, Hamdi̇; İnalöz, H. Serhat; Doğanay, Selim; Türköz, Yusuf; Özerol, İbrahim Halil

doi:10.1046/j.1365-4362.2003.01688.x

Cited by 90 publications

(64 citation statements)

References 38 publications

(48 reference statements)

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“…Other studies have also shown an increase in NO levels of urine and synovial fluid in BD [19][20]. Vascular endothelial growth factor levels are shown to be increased in patients with BD [21] and it has been suggested to be caused by an increase in NO. According to our data low levels of ADMA may also contribute to NO increase in BD.…”

Section: Resultsmentioning

confidence: 91%

Asymmetric dimethylarginine levels in Behçet's Disease

Öztürk¹,

Vatansev²,

Taner³

et al. 2014

Turk J Bioch

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Objective: Behçet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods:The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging. and myocardial infarction; (c) diabetes mellitus, renal and hepatic diseases, active infectious diseases; (d) other known chronic inflammatory diseases. The body mass index (BMI) and blood pressure were recorded. It was also noted that whether the subjects smoke or not. Each subject was evaluated in active and inactive disease periods. At the time of examination, the presence of two or more of the following Behçet's clinical features was considered as active disease: Oral ulceration, genital ulceration, skin lesions, ocular lesions, active major vessel disease, and active major organ involvement including active gastrointestinal or neurological lesions. Subjects with one lesion or lesion-free period were regarded in inactive disease period.The protocol was approved by the Ethics Committee of the Selcuklu Medical Faculty (24.06.2010Faculty (24.06. -2010) and all subjects volunteered for the trial and written consent was obtained according to the Declaration of Helsinki.Blood samples were centrifuged with 2000 x g for 10 min at +4°C, followed by serum removal for assays. All serum samples were stored at -80°C until analysis.Measurements of ADMA, arginine and citrulline levels were accomplished by high performance liquid chromatography (HPLC), using the method described by Taner et al [17]. In brief, 20 mg 5-sulfosalisilic acid was added to 1 ml serum, and the mixture was left in an ice bath for 10 min. The precipitated protein was removed by centrifugation at 2,000×g for 10 min. Ten µl of the supernatant was filtered using a 0.45-μm pore size filter, which was then mixed with 100 μl derivatization reagent [prepared by dissolving ...

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Section: Resultsmentioning

confidence: 91%

Asymmetric dimethylarginine levels in Behçet's Disease

Öztürk¹,

Vatansev²,

Taner³

et al. 2014

Turk J Bioch

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“…We conclude that, like serum VEGF, 7 plasma VEGF levels are useful in diagnosing POEMS syndrome and Castleman disease and can help differentiate from other plasma cell dyscrasias as well as diseases presenting with similar features (ie, peripheral neuropathy, connective tissue disease, and vasculitis). Elevated plasma VEGF levels have been described in several types of cancer, 11 rheumatologic illnesses, 12,13 renal diseases, 14 and in sepsis. 14 The sensitivity of plasma VEGF increases further when used in the appropriate clinical setting, and after accounting for acute illnesses, and medications (corticosteroid use).…”

Section: Resultsmentioning

confidence: 99%

The utility of plasma vascular endothelial growth factor levels in the diagnosis and follow-up of patients with POEMS syndrome

D’Souza¹,

Hayman²,

Buadi³

et al. 2011

Blood

182

174

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The POEMS syndrome is associated with elevated vascular endothelial growth factor (VEGF) levels. Several studies have compared serum VEGF levels between POEMS patients and other disease entities showing higher serum VEGF in POEMS syndrome; however, it is unknown whether serum levels are reliable and reproducible given variable platelet release of VEGF. We therefore compared plasma levels of VEGF in 29 patients with POEMS syndrome with those of other disorders (n = 76). We demonstrated that plasma VEGF levels are useful in differentiating POEMS from other plasma cell dyscrasias, neuropathic processes, and multisystem illnesses. Plasma VEGF is also useful in monitoring disease activity after treatment and correlates with clinical improvements better than hematologic response.

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“…Our comprehensive study of proteomics led to the possibility that monitoring the level of serum ORM1 could be clinically useful for the screening and diagnosis of PTR patients. α-1-antitrypsin (SERPINA1) is an inhibitor of serine proteases principally secreted by hepatocytes, but also by monocytes, neutrophils, macrophages and alveolar epithelial cells, and plays a critical role in modulating host immunity, inhibiting T lymphocyte-mediated antitumor function and thereby accelerated tumor proliferation, and metastasis (3)(4)(5). Moreover, there have been numerous studies documenting a link between SERPINA1 and various cancers, although for most the mechanism for the linkage is unclear.…”

Section: Discussionmentioning

confidence: 99%

Identification of proteomic and metabolic signatures associated with chemoresistance of human epithelial ovarian cancer

Wang

Yin

et al. 2016

International Journal of Oncology

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Abstract. Emerging drug resistance in epithelial ovarian cancer (EOC) thwarted progress in platinum-based chemotherapy, resulting in increased mortality, morbidity and healthcare costs. The aim of this study was to detect the responses induced by chemotherapy at protein and metabolite levels, and to search for new plasma markers that can predict resistance to platinum-based chemotherapy in EOC patients, leading to improved clinical response rates. Serum samples were collected and subjected to proteomic relative quantitation analysis and metabolomic analysis. Differentially expressed proteins and metabolites were subjected to bioinformatics and statistical analysis. Proteins that played a key role in platinum resistance were validated by western blotting and enzyme-linked immunosorbent assay (ELISA). Metabolites that were the main contributors to the groups and closely with clinical characteristics were identified based on the database using nuclear magnetic resonance (NMR). In total, 248 proteins from two independent experiments were identified using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic approach. Among them, FN1, SERPINA1, GPX3 and ORM1 were chosen for western blotting and ELISA validation. Platinum resistance likely associated with differentially expressed proteins and FN1, SERPINA1 and ORM1 may play a positive role in chemotherapy. HPLC-MS analysis of four groups revealed a total of 25,800 metabolic features, of which six compounds were chosen for candidate biomarkers and identified based on the database using NMR. The metabolic signatures of normal control (NC), platinum-sensitive (PTS) and platinum-resistant (PTR) groups were clearly separated from each other.Those findings may provide theoretical clues for the prediction of chemotherapeutic response and reverse of drug resistance, even lead to novel targets for therapeutic intervention.

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Vascular endothelial growth factor levels are increased and associated with disease activity in patients with Behçet's syndrome

Abstract: VEGF may participate in the course of Behçet's syndrome, especially in the active stage, and elevated levels of VEGF may be an additional risk factor for the development of ocular disease, contributing to poor visual outcome.

Cited by 90 publications

References 38 publications

Asymmetric dimethylarginine levels in Behçet's Disease

Asymmetric dimethylarginine levels in Behçet's Disease

The utility of plasma vascular endothelial growth factor levels in the diagnosis and follow-up of patients with POEMS syndrome

Identification of proteomic and metabolic signatures associated with chemoresistance of human epithelial ovarian cancer

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