Vascular endothelial growth factor‐D modulates oxidant–antioxidant balance of human vascular endothelial cells

Papiewska-Pająk, Izabela; Balcerczyk, Aneta; Stec-Martyna, Emilia; Koziołkiewicz, Wiktor; Boncela, Joanna

doi:10.1111/jcmm.13045

Cited by 10 publications

(11 citation statements)

References 67 publications

(74 reference statements)

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“…VEGF-D, an angiogenic and lymphangiogenic glycoprotein, can facilitate tumor growth and distant organ metastasis [11] . Accumulated evidences have proved the expression of VEGF-D is usually connected with tumor metastasis and poor patient outcome.…”

Section: Discussionmentioning

confidence: 99%

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Consecutive Observation of Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

Du¹,

Kong²,

Yang³

et al. 2020

Preprint

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Background：Lymphogenous metastasis, one of the most common dissemination routes for ovarian carcinoma, predicts a poor prognosis and relates to most cancer-related death. Now there were no effective therapy and control methods for ovarian carcinoma. Hence, it is necessary to build an animal model of lymph node metastasis in ovarian cancer to seek for the tools to find effective treatments. Our purpose of this study was to investigate the tumor cell dissemination of ovarian carcinoma to the gradient lymph nodes of nude mice and its possible mechanism. Methods: The mice models of VEGF-D over-expressed were built and the tumor growth and sentinel lymph nodes were evaluated weekly, while the visible lymph nodes and tumor masses were excised for histological examination using HE examination. Then Evan’s Blue was conducted to observe the unveil lymphatic network. Subsequently, immunohistochemistry was performed to check the expression of relative genes. Meanwhile, microvessel counting was performed within the tumor tissues and measured using computer assisted morphometric analysis.Results: The over-expression of VEGF-D promoted the tumor growth of ovarian carcinoma, facilitated the hyperplasia of tumor lymphatic and increased the intratumoral lymphatic vessel density. Besides, the up-regulation of VEGF-D induced the expression of MMP-2, which might be the underlying mechanism for the lymph metastasis in ovarian cancer. Conclusion: it was a step-by-step progression from the inoculation of cancer cells, to the proliferation of the primary tumors, and then to the lymphatic metastases, in which VEGF-D and MMP-2 played vital roles.

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Section: Discussionmentioning

confidence: 99%

“…Vascular endothelial growth factor D (VEGF-D) is a member of VEGF family, which is the typical lymphangiopoiesis factor, which is often correlated with tumor metastasis and poor patient outcome [11] .…”

Section: Introductionmentioning

confidence: 99%

Consecutive Observation of Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

Du¹,

Kong²,

Yang³

et al. 2020

Preprint

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show abstract

“…In our study, we established the lymphogeneous high metastasis animal model with SKOV-3 cells transfected with VEGF-D [16] , and explored the underlying mechanisms of the lymph metastasis. VEGF-D, an angiogenic and lymphangiogenic glycoprotein, can facilitate tumor growth and distant organ metastasis [11] . Accumulated evidences have proved the expression of VEGF-D is usually connected with tumor metastasis and poor patient outcome.…”

Section: Discussionmentioning

confidence: 99%

The Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

Kong

Yang

et al. 2020

Preprint

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Background：Lymphogenous metastasis, one of the most common dissemination routes for ovarian carcinoma, predicts a poor prognosis and relates to most cancer-related death. Now there were no effective therapy and control methods for ovarian carcinoma. Hence, it is necessary to build an animal model of lymph node metastasis in ovarian cancer to seek for the tools to find effective treatments. Our purpose of this study was to investigate the tumor cell dissemination of ovarian carcinoma to the gradient lymph nodes of nude mice and its possible mechanism. Methods: The mice models of VEGF-D over-expressed were built and the tumor growth and sentinel lymph nodes were evaluated weekly, while the visible lymph nodes and tumor masses were excised for histological examination using HE examination. Then Evan’s Blue was conducted to observe the unveil lymphatic network. Subsequently, immunohistochemistry was performed to check the expression of relative genes. Meanwhile, microvessel counting was performed within the tumor tissues and measured using computer assisted morphometric analysis.Results: The over-expression of VEGF-D promoted the tumor growth of ovarian carcinoma, facilitated the hyperplasia of tumor lymphatic and increased the intratumoral lymphatic vessel density. Besides, the up-regulation of VEGF-D induced the expression of MMP-2, which might be the underlying mechanism for the lymph metastasis in ovarian cancer. Conclusion: It was a step-by-step progression from the inoculation of cancer cells, to the proliferation of the primary tumors, and then to the lymphatic metastases, in which VEGF-D and MMP-2 played vital roles.

show abstract

“…Vascular endothelial growth factor D (VEGF-D) is a member of VEGF family, which is the typical lymphangiopoiesis factor, and is often correlated with tumor metastasis and poor patient outcome [18] .…”

Section: Introductionmentioning

confidence: 99%

Consecutive Observation of Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

Kong

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background：Lymphogenous metastasis, one of the most common dissemination routes for ovarian carcinoma, predicts a poor prognosis and relates to most cancer-related death. Now there were no effective therapy and control methods for ovarian carcinoma. Hence, it is necessary to build an animal model of lymph node metastasis in ovarian cancer to seek for the tools to find effective treatments. Our purpose of this study was to investigate the tumor cell dissemination of ovarian carcinoma to the gradient lymph nodes of nude mice and its possible mechanism.Methods: The mice models of VEGF-D over-expressed were built, and the tumor growth and sentinel lymph nodes were evaluated weekly, while the visible lymph nodes and tumor masses were excised for histological examination using HE examination. Then Evan’s Blue was conducted to observe the unveil lymphatic network. Subsequently, immunohistochemistry (IHC) was performed to check the protein expression of relative genes. Meanwhile, microvessel counting was performed within the tumor tissues and measured using computer assisted morphometric analysis.Results: The over-expression of VEGF-D promoted the tumor growth of ovarian carcinoma, facilitated the hyperplasia of tumor lymphatic and increased the intratumoral lymphatic vessel density. Besides, the up-regulation of VEGF-D induced the expression of MMP-2, which might be the underlying mechanism of the lymph metastasis in ovarian cancer.Conclusion: It was a step-by-step progression from the inoculation of cancer cells to the proliferation of the primary tumors, and then to the lymphatic metastases, in which VEGF-D and MMP-2 played vital roles.

show abstract

Vascular endothelial growth factor‐D modulates oxidant–antioxidant balance of human vascular endothelial cells

Cited by 10 publications

References 67 publications

Consecutive Observation of Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

Consecutive Observation of Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

The Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

Consecutive Observation of Dynamic Metastases of Human Ovarian Carcinoma to Grading Lymph Nodes in Mouse Model

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