Vascular endothelial growth factor and its soluble receptor in infants with congenital cardiac disease

Pohl-Schickinger, A.; Koehne, Petra; Schmitz, Thomas; Schmitt, Katharina; Hübler, Michael; Redlin, Matthias; Berger, Felix; Stiller, Brigitte

doi:10.1017/s1047951110000545

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…CD133 present star like and distribute on vessels marked by CD31, suggesting that angiogenesis might be promoted by EPCs marked by CD133. VA also increased the content of VEGF in serum, which can stimulate angiogenesis (Pohl-Schickinger et al, 2010). The morphology of vascular endothelial cells in the VA treated group was better than that in the MI group, which is consistent with previous work, that is, VA proteins had protective effects on the cardiac microvascular endothelial cells challenged with ischemia-hypoxia (Xiao et al, 2017b).…”

Section: Discussionmentioning

confidence: 99%

Velvet Antler Mobilizes Endothelial Progenitor Cells to Promote Angiogenesis and Repair Vascular Endothelial Injury in Rats Following Myocardial Infarction

Wang

Mao

et al. 2019

Front. Physiol.

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Objective: This investigation examined the effect of velvet antler (VA) on endothelial progenitor cells (EPCs) and the associated effects to promote angiogenesis and repair vascular endothelial injury in rats with myocardial infarction (MI).Methods: VA was analyzed by liquid chromatography-mass spectrometry. Male Sprague Dawley rats were randomly divided into four groups: sham, MI, VA, and VA + DAPT (gamma-secretase inhibitor IX, a specific blocker of the Notch signaling pathway) group. The rats underwent ligation of the left anterior descending coronary artery for the establishment of MI. Sham-operated rats were used as controls. Blood was taken from the orbital plexus on the first and third days after the operation, and all rats were euthanized on the 7th day after surgery. The blood samples were used to detect the contents of circulating endothelial progenitor cells (CEPCs) and vascular endothelial growth factor (VEGF). Echocardiography was used to test the cardiac function. Cardiac tissue was used for immunohistochemistry and electron microscope, and the marginal zone of the MI tissue was used for western blot and reverse transcription-quantitative polymerase chain reaction.Results: The number of basically qualitative metabolites is 445. Among them, there are 74 substances with relative content greater than 0.05%. VA increased the concentration of CEPCs and VEGF in serum, CD133 content and microvessel density (MVD), and protected the morphology of microvascular endothelial cells in the marginal area of MI at 7 days post-MI surgery. CEPCs and MVD in the VA +DAPT group were lower than those of VA group. VA increased the protein expressions of Jagged-1, Notch1, NICD and HES1, and the mRNA expressions of Hes1 and Hey2, while some of the effects could be suppressed by DAPT.Conclusion: These results suggest that VA promotes the mobilization of EPCs to promote angiogenesis and repair vascular endothelial cell damage in post-MI rats, and these effects may be due to activation of the Notch signal pathway.

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Section: Discussionmentioning

confidence: 99%

Velvet Antler Mobilizes Endothelial Progenitor Cells to Promote Angiogenesis and Repair Vascular Endothelial Injury in Rats Following Myocardial Infarction

Wang

Mao

et al. 2019

Front. Physiol.

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“…Following MI, the formation of collateral circulation requires the stimulation of quiescent endothelial cells by various growth factors, including VEGF and bFGF. The role of VEGF and bFGF in angiogenesis is well understood ( 30 , 31 ). VEGF proteins are homodimeric, with two subunits of ~120–200 amino acids in length, and bind to Fms-like tyrosine and fetal liver kinase-1/kinase insert domain receptor on the endothelial cells ( 32 ) to stimulate proliferation of the endothelial cells to form new microvessels ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

“…The role of VEGF and bFGF in angiogenesis is well understood ( 30 , 31 ). VEGF proteins are homodimeric, with two subunits of ~120–200 amino acids in length, and bind to Fms-like tyrosine and fetal liver kinase-1/kinase insert domain receptor on the endothelial cells ( 32 ) to stimulate proliferation of the endothelial cells to form new microvessels ( 31 ). bFGF, however, mediates the expression of integrins, enhances cell adhesion and migration of vascular endothelial and smooth muscle cells, promotes the formation of new blood vessels and increases collateral circulation ( 22 , 23 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction

Zhang

Jiang

et al. 2015

Molecular Medicine Reports

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The aim of the present study was to evaluate the effect of astragalosides (ASTs) on angiogenesis, as well as the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) following myocardial infarction (MI). MI was induced in rats by ligation of the left coronary artery. Twenty-four hours after surgery, the rats were divided into low-dose, high-dose, control and sham surgery groups (n=8 per group). The low- and high-dose groups were treated with ASTs (2.5 and 10 mg/kg/day, respectively, via intraperitoneal injection), while, the control and sham surgery group rats received saline. Serum levels, and mRNA and protein expression levels of VEGF and bFGF, as well as the microvessel density (MVD) were determined four weeks post-treatment. Twenty-four hours post-surgery, VEGF and bFGF serum levels were observed to be comparable between the groups; while at four weeks, the VEGF and bFGF levels were higher in the AST-treated rats (P<0.01). Similarly, VEGF and bFGF mRNA and protein expression levels were higher following AST treatment (P<0.05). No difference in VEGF mRNA expression between the low- and high-dose groups was noted, however, an increase in the bFGF expression levels was detected in the high-dose group. Newly generated blood vessels were observed following MI, with a significant increase in MVD observed in the AST-treated groups (P<0.05). AST promotes angiogenesis of the heart and increases VEGF and bFGF expression levels. Thus, it is hypothesized that increased VEGF and bFGF levels may contribute to the AST-induced increase in angiogenesis in rat models of MI.

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The use of heparin, bFGF, and VEGF 145 grafted acellular vascular scaffold in small diameter vascular graft

et al. 2018

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We aim to test the application of heparin, bFGF, and VEGF 145 grafted acellular vascular scaffold in small diameter vascular graft. The amount of bFGF and VEGF 145 were determined by ELISA. Femoral artery transplantation was performed. Mechanical strength of acellular vascular scaffolds was determined. Angiography was performed for blood vessel patency. Factor VIII and α2-actin expression was detected by immunohistochemistry. bFGF and VEGF 145 had stable release at 60 and 70 days in vitro, and the release rate of VEGF 145 was slightly slower than that of bFGF. After transplantation, 9 months of the vascular patency rate was 100% at 1, 3, and 9 months, and, was up to 90% at 18 months, while the patency rate in group with grafted heparin only at 1-month was 60%, at 3-month was 40%, at 9-month was 15%, and at 18-month was 10%. The blood vessels taken after 18 months had no significant difference in the mechanical properties between the transplanted and the natural vessels. Positive expression of factor VIII and α2-actin was observed. The heparinized and bFGF and VEGF 145 grafted allogeneic vascular acellular scaffolds are preliminarily obtained, which show good biocompatibility and patency and are of great importance for small diameter vascular graft. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 00B: 000-000, 2018.

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Vascular endothelial growth factor and its soluble receptor in infants with congenital cardiac disease

Cited by 4 publications

References 11 publications

Velvet Antler Mobilizes Endothelial Progenitor Cells to Promote Angiogenesis and Repair Vascular Endothelial Injury in Rats Following Myocardial Infarction

Velvet Antler Mobilizes Endothelial Progenitor Cells to Promote Angiogenesis and Repair Vascular Endothelial Injury in Rats Following Myocardial Infarction

Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction

The use of heparin, bFGF, and VEGF 145 grafted acellular vascular scaffold in small diameter vascular graft

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