Vascular endothelial growth factor A and its two receptors in human preantral follicles from fetuses, girls, and women

Abir, Ronit; Ao, Asangla; Zhang, Xiao Yun; Garor, Roni; Nitke, Shmuel; Fisch, Benjamin

doi:10.1016/j.fertnstert.2009.01.111

Cited by 27 publications

(20 citation statements)

References 27 publications

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“…Consisted with our results, previous studies revealed that VEGF is expressed in oocyte of ostrich [22], rat [23], ovine [24], porcine [25] and human [26]. In the intervention groups, with the increase in FSH concentrations, the expression of VEGF increased gradually.…”

Section: Discussionsupporting

confidence: 87%

Optimal FSH usage in revascularization of allotransplanted ovarian tissue in mice

Zheng

Hei

et al. 2017

J Ovarian Res

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BackgroudOvarian transplantation is a useful method for preserving the fertility of young women with cancer who undergo radiotherapy and chemotherapy. Follicle-stimulating hormone (FSH) is use to protect transplanted ovarian tissues from ischemia injury through promoting revascularization after transplantation, but the side effect of high level FSH is ovarian overstimulation leading to substantial follicular loss. In this study, we investigated the optimal usage of FSH on revascularization in the in vitro cultured ovarian tissues before and after transplantation.ResultsFSH mainly exhibited an additive response in the gene and protein expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and follicle stimulating hormone receptor (FSHR) with its raised concentrations (0.15 IU/ml, 0.30 IU/ml and 0.60 IU/ml) and prolonged treatment (3 h, 6 h, 12 h, 24 h). The concentrations with 0.60 IU/ml FSH could obviously promoted the expression of VEGF, bFGF and FSHR, but under this concentration FSH could also overstimulated the ovarian tissue leading to follicular loss. With the increase of culture time, the gene and protein expression of VEGF and bFGF both were up-regulated in all of the FSH added groups, but FSHR expression decreased when culture time exceeded 12 h. So we chose 0.30 IU/ml FSH added concentration and 6 h culture time as the FSH usage condition in functional revascularization verification experiment, and found that under this condition FSH promoted 2.5 times increase of vascular density in treated group than in control group after ovarian tissues transplantation.ConclusionOvarian intervention with 0.30 IU/ml FSH for 6 h is an optimal FSH usage condition which could accelerate the revascularization in the allotransplanted ovarian tissue and can not produce ovarian overstimulation.

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Section: Discussionsupporting

confidence: 87%

Optimal FSH usage in revascularization of allotransplanted ovarian tissue in mice

Zheng

Hei

et al. 2017

J Ovarian Res

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“…VEGF is a 34-to 42-kDa protein, and there are seven members of the VEGF family. VEGF interacts with its receptors present in granulosa and theca cells to act as a mitogenic factor in developing goat (Bruno et al 2009) and preantral human follicles (Abir et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles

Bui

Nguyễn

Asako

et al. 2017

Reprod. Fertil. Dev.

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The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3 mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6 mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200 ng mL -1 VEGF during the first 20 h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200 ng mL -1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200 ng mL -1 during the first 20 h of IVM.Additional keyword: pig. T. M. T. Bui et al. Effect of VEGF on IVM of porcine oocytesOvaries contain a large number of small follicles, but the meiotic and developmental competence of oocytes from these follicles is quite low. In the present study we demonstrated that the addition of 200 ng mL -1 vascular endothelial growth factor to the IVM medium increased the maturation rate of porcine oocytes from small follicles and that blastocyst formation following parthenogenetic activation also increased. These findings may contribute to efficient animal production and human assisted reproductive technology.

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“…Vascular endothelial growth factor (VEGF), specifically VEGF-A, is a potent angiogenic factor that stimulates vascular endothelial cell survival, proliferation, migration, and differentiation. Moreover, some data suggest that VEGF-A appears to be involved in folliculogenesis with the presence of VEGF-A receptors in granulosa cells [ 18 ]. VEGF is a disulfide-bonded dimeric glycoprotein that is encoded by a gene that contains 8 exons.…”

Section: Introductionmentioning

confidence: 99%

Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice

Henry

Labied

Fransolet

et al. 2015

Reprod Biol Endocrinol

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BackgroundAggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement.MethodsFrozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies.ResultsWe found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3 days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53% and 29% of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. CD31 positive murine blood vessels were observed in 40% of the VEGF165 transplants compared with 21% of the controls. After 3 weeks, the density of murine vessels was significantly higher in the VEGF165 group.ConclusionThe encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.

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Vascular endothelial growth factor A and its two receptors in human preantral follicles from fetuses, girls, and women

Cited by 27 publications

References 27 publications

Optimal FSH usage in revascularization of allotransplanted ovarian tissue in mice

Optimal FSH usage in revascularization of allotransplanted ovarian tissue in mice

Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles

Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice

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