Vascular endothelial growth factor: A new member of the platelet-derived growth factor gene family

Tischer, Edmund; Gospodarowicz, Denis; Mitchell, R.; Silva, Maria João; Schilling, James; Lau, Kenneth; Crisp, Tracey; Fiddes, John C.; Abraham, Judith A.

doi:10.1016/0006-291x(89)92729-0

Cited by 305 publications

(183 citation statements)

References 21 publications

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“…The 5.1 kb VEGF promoter fragment, containing the ®rst two exons of VEGF and the 3.4 kb sequence upstream to the translation initiation site (Tischer et al, 1991), was kindly provided by JA Abraham. The 5.1 kb EcoRI fragment was digested with AccI and NheI and the resulting 2068 bp VEGF promoter fragment (72018 to +50, relative to the transcriptional start site) was cloned into AccI and XhoI of the promoterless luciferase reporter vector pAH 1409 (Hecht, 1990) to generate pLuc 2068.…”

Section: Vegf Promoter-luciferase Constructsmentioning

confidence: 99%

“…One or several of these signaling pathways may lead to induction of VEGF gene expression upon autocrine and/or paracrine PDGFstimulation of cells. Sequence analysis of the VEGF gene promoter revealed several potential binding sites for the transcription factors AP-1, AP-2, and Sp1 (Tischer et al, 1991) which are candidate mediators of the PDGF signal.…”

Section: Introductionmentioning

confidence: 99%

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Sp1 recognition sites in the proximal promoter of the human vascular endothelial growth factor gene are essential for platelet-derived growth factor-induced gene expression

Finkenzeller

Sparacio

Technau³

et al. 1997

Oncogene

182

170

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Stimulation of NIH3T3 cells with platelet-derived growth factor (PDGF)-BB enhances expression of vascular endothelial growth factor (VEGF), an endothelial cell-speci®c mitogen and a key mediator of tumor angiogenesis. Here, we identi®ed cis-acting VEGF promoter elements and trans-acting factors which are involved in PDGF-stimulated VEGF expression. By 5'-deletion and transient transfection analysis, a G+C-rich region at 785 to 750 of the human VEGF promoter was shown to be necessary and su cient for both PDGF inducible and basal expression. The region contains three potential recognition sites for Sp1 transcription factors, which overlap with two Egr-1 sites. Mutations that abolish the ability of Sp1 to interact with the VEGF promoter element also abrogate expression induced by PDGF. Mutations of the potential Egr-1 binding sites did not a ect PDGF responsiveness. Gel shift and antibody supershift analyses showed that Sp1 and Sp3 interact constitutively with the VEGF promoter element. Our data strongly suggest that enhanced VEGF gene expression in PDGF-induced NIH3T3 cells is mediated by Sp1 and/or Sp3 transcription factors bound to the 785 to 750 promoter region of the VEGF gene.

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Section: Vegf Promoter-luciferase Constructsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sp1 recognition sites in the proximal promoter of the human vascular endothelial growth factor gene are essential for platelet-derived growth factor-induced gene expression

Finkenzeller

Sparacio

Technau³

et al. 1997

Oncogene

182

170

View full text Add to dashboard Cite

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“…The VEGF gene undergoes alternative splicing to produce multiple functional isoforms, such as 121, 165 and 189 in human [3] and 120, 164, and 188 in the mouse [4]. Fibrosarcoma cell lines have been developed to express single isoforms of VEGF and used to study their roles in tumour vascularisation.…”

Section: Introductionmentioning

confidence: 99%

Microflow of fluorescently labelled red blood cells in tumours expressing single isoforms of VEGF and their response to vascular targeting agents

Akerman

Reyes-Aldasoro

Fisher

et al. 2011

Medical Engineering & Physics

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“…Bone morphogenetic proteins upregulate the expression of vascular endothelial growth factor, a member of the PDGF superfamily [30]. In clinical and animal studies, BMPs promote fracture healing in a normal setting and in pathologic conditions, including infection [4,5,7,14,35].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Protein-1 Overcomes Inhibition of Fracture Healing in the Diabetic Rat: A Pilot Study

Kidder

Chen

Schmidt

et al. 2008

Clin Orthop Relat Res

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Type I diabetes mellitus inhibits fracture healing and leads to an increase in complications. As a pilot study, we used a closed fracture model in the diabetic rat to address the question of whether osteogenic protein-1 (OP-1) in a collagen carrier can overcome this inhibition by increasing the area of the newly mineralized callus and femoral torque to failure compared with diabetic animals with fractures treated without OP-1. Diabetes was created in 54 rats by injection of streptozotocin. After 2 weeks, a closed femur fracture was created using a drop-weight impaction device. Each fracture site was immediately opened and treated with or without 25 lg OP-1 in a collagen carrier. Animals were euthanized after 2 or 4 weeks. Fracture healing was assessed by callus area from highresolution radiographs, callus strength from torsional failure testing, and undecalcified histologic analysis. The area of newly mineralized callus was greater in diabetic animals treated with 25 lg OP-1/carrier compared with diabetic animals with untreated fractures and with fractures treated with carrier alone. This increase in callus area did not translate into an equivalent increase in torque to failure. Osteogenic protein-1 showed some evidence of overcoming the inhibition of fracture healing in the diabetic rat.

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Vascular endothelial growth factor: A new member of the platelet-derived growth factor gene family

Cited by 305 publications

References 21 publications

Sp1 recognition sites in the proximal promoter of the human vascular endothelial growth factor gene are essential for platelet-derived growth factor-induced gene expression

Sp1 recognition sites in the proximal promoter of the human vascular endothelial growth factor gene are essential for platelet-derived growth factor-induced gene expression

Microflow of fluorescently labelled red blood cells in tumours expressing single isoforms of VEGF and their response to vascular targeting agents

Osteogenic Protein-1 Overcomes Inhibition of Fracture Healing in the Diabetic Rat: A Pilot Study

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