Vascular effects and antioxidant activity of two Combretum species from Democratic Republic of Congo

Manga, Francine Nsuadi; Khattabi, Charaf El; Fontaine, Jeanine; Berkenboom, Guy; Duez, Pierre; Nzunzu, José Lami; Pochet, Stéphanie

doi:10.1016/j.jep.2012.04.039

Cited by 16 publications

(12 citation statements)

References 44 publications

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“… 17 , 18 Substances identified in other Combretum genera members are also capable of inducing EDR in rat aortas; however the concentrations required are higher than those of ECL; for example, 5 to 80µg/mL (to mollic acid glucoside isolated from C. molle leaf) and EC 50 of 3.9µg/mL and 9.5µg/mL for methanolic extract of C. celastroides leaves, and C. racemosum roots respectively. 3 , 4 As in the rat aorta, 1.5µg/mL of ECL causes complete relaxation of PE-induced contractions the vasodilator in the extract of compounds present in ECL are unlikely to be related to those already described in other members of the genus Combretum .…”

Section: Discussionmentioning

confidence: 89%

“…In a bioactivity-based screening of the potential effects of the lyophilized hydroalcoholic extract of Combretum leprosum (ECL) bark on visceral and vascular smooth muscles, it was observed that the extract caused a potent endothelium-dependent relaxation (EDR) of rabbit thoracic aorta rings. Although some substances isolated from related species of the Combretum genus have been shown to cause EDR in rat aorta (mollic acid glucoside, isolated from Combretum molle ) as well as methanolic fractions rich in flavonoids from Combretum celastroides and Combretum racemosum, 3 , 4 all previously reported effects of flower, leaf and/or root ECL did not include any cardiovascular bioactivity.…”

Section: Introductionmentioning

confidence: 94%

“…Em uma triagem baseada na bioatividade dos possíveis efeitos do extrato hidroalcoólico liofilizado da casca de Combretum leprosum (ECL) em músculos lisos viscerais e vasculares, observou-se que o extrato causou um potente relaxamento dependente do endotélio ( endothelium-dependent relaxation - EDR)dos anéis da aorta torácica de coelho. Embora tenha sido descrito que substâncias isoladas de espécies relacionadas do gênero Combretum causam EDR na aorta de ratos (glicosídeo ácido móllico isolado do Combretum molle ), bem como frações metanólicas ricas em flavonoides de Combretum celastroides e Combretum racemosum , 3 , 4 todos os efeitos de extratos de flores, folhas e/ou raízes de ECL previamente descritos não incluem nenhuma bioatividade cardiovascular.…”

Section: Introductionunclassified

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Long-lasting endothelium-dependent relaxation of isolated arteries caused by an extract from the bark of Combretum leprosum

Filho

Cavalcanti

Passaglia

et al. 2015

Einstein (São Paulo)

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Objective To describe and to characterize the relaxing effect of an extract of the bark of Combretum leprosum on isolated arterial rings from different animals.Methods Rings (3 to 4mm) from rabbit, rat, or porcine arteries rings were suspended in an organ bath (Krebs, 37°C, 95%O2/5%CO2) to record isometric contractions. After the stabilization period (2 to 3 hours) contractions were induced by the addition of phenylephrine (0.1 to 0.3µM) or U46619 (10 to 100nM), and Combretum leprosum extract was added on the plateau of the contractions. Experiments were performed to determine the potency, duration, reversibility, and to get insights on the potential mechanism involved in extract-induced relaxations.Results In all rings tested, Combretumleprosum extract (1.5μg/mL) was able to cause relaxations, which were strictly endothelium-dependent. In rabbit or rat thoracic aorta rings, the relaxations were reversed by vitamin B12a or L-NG-nitroarginine. In porcine right coronary arteries and rabbit abdominal aorta, extract caused both L-NG-nitroarginine-sensitive and L-NG-nitroarginine-resistant relaxations. In rabbit thoracic aorta, the extract was relatively potent (EC50=0.20µg/mL) and caused relaxations; intriguingly the endothelium continued to produce relaxing factors for a long period after removing the extract. The magnitude of extract-induced relaxations was significantly reduced in the absence of extracellular Ca2+; in addition, the TRPs channels blocker ruthenium red (10µM) was able to revert extract-induced relaxations. Phytochemical analyses indicated that the extract was rich in polyphenol-like reacting substances.Conclusions Combretum leprosum extract contains bioactive compounds capable of promoting Ca2+-dependent stimulation of endothelial cells which results in a prolonged production of relaxing factors.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 94%

Section: Introductionunclassified

See 1 more Smart Citation

Long-lasting endothelium-dependent relaxation of isolated arteries caused by an extract from the bark of Combretum leprosum

Filho

Cavalcanti

Passaglia

et al. 2015

Einstein (São Paulo)

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“…Recent studies have shown that plants of the Combretum genus have antidiabetic activity [9,10], as well as in vivo antioxidant activity [11,12]. Recently, study of Dechandt and collaborators [13] observed a decrease in glycemia, glycosuria and urinary urea levels in streptozotocin-diabetic rats treated for 21 days with the ethanolic extract of the flowers of Combretum lanceolatum (ClEtOH).…”

Section: Introductionmentioning

confidence: 99%

Changes in the Oxidative Stress Biomarkers in Liver of Streptozotocin-diabetic Rats Treated with Combretum lanceolatum Flowers Extract

Dechandt

Souza

Siqueira

et al. 2014

BJPR

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“…Water or organic extracts of C. racemosum leaves also showed significant antimicrobial activities against various pathogenic microorganisms [10, 11]. Furthermore, the antiinflammatory, vasorelaxant, and trypanocidal properties of the C. racemosum extracts have been validated in recent pharmacological studies [12, 13]. Preliminary phytochemical screening of C. racemosum extracts indicated the presence of alkaloids, steroids, cardiac glycosides, saponins and tannins [9].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum

Schepetkin

Kouakou

Yapi³

et al. 2013

International Immunopharmacology

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Extracts of leaves of different species of the genus Combretum have been used historically to treat a variety of medicinal problems. However, little is known about the active components conferring therapeutic properties to these extracts. In the present studies, we evaluated biochemical properties and immunomodulatory activity of polysaccharides isolated from the leaves of Combretum racemosum. Water-soluble polysaccharides from leaves of C. racemosum were extracted and fractionated by DEAE-cellulose and Diaion HP-20 to obtain a Diaion-bound fraction, designated Combretum polysaccharide-acidic bound or CP-AB, which was eluted with methanol, and an unbound fraction, designated as CP-AU. Molecular weight determination, sugar analysis, and other physical and chemical characterization of the fractions were performed. Fraction CP-AU (mol. weight 5.0 kDa) contained type II arabinogalactan and had potent immunomodulatory activity, inducing the production of interleukin (IL)-1β, -6, -10, and tumor necrosis factor-α (TNF-α) by human peripheral blood mononuclear cells (PBMC) and MonoMac-6 monocytic cells. Likewise, intraperitoneal administration of CP-AU increased in vivo serum levels of IL-6 and monocyte chemoattractant protein-1 (MCP-1) in mice. CP-AU-induced secretion of TNF-α in PBMC was prevented by Toll-like receptor 4 (TLR4) antagonist LPS-RS. Treatment with CP-AU induced phosphorylation of Akt2, Akt3, GSK-3β, HSP27, mTOR, and all p38 MAPK isoforms (α, β, δ, and γ), as well as stimulation of AP-1/NF-κB transcriptional activity. In addition, CP-AU effectively agglutinated erythrocytes from several species, including human, mouse, and rabbit. In contrast, fraction CP-AB was inactive in all biological tests, including cytokine production and hemagglutination. These data suggest that at least part of the beneficial therapeutic effects reported for the water extracts of leaves from C. racemosum are due to modulation of leukocyte functions.

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Vascular effects and antioxidant activity of two Combretum species from Democratic Republic of Congo

Cited by 16 publications

References 44 publications

Long-lasting endothelium-dependent relaxation of isolated arteries caused by an extract from the bark of Combretum leprosum

Long-lasting endothelium-dependent relaxation of isolated arteries caused by an extract from the bark of Combretum leprosum

Changes in the Oxidative Stress Biomarkers in Liver of Streptozotocin-diabetic Rats Treated with Combretum lanceolatum Flowers Extract

Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum

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