Vascular Development of the Heart

Tomanek, Robert J.; Yue, Xinping; Zheng, Wei

doi:10.1007/978-1-4612-0109-0_7

Cited by 6 publications

(6 citation statements)

References 104 publications

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“…The in situ experiments were motivated by our hypothesis that the capillary plexus at the root of the truncus arteriosus, or its associated cells, is unique in that it penetrates the aorta at the coronary sinuses to form the roots of the two main coronary arteries (Ratajska and Fiejka, 1999; and recent review by Tomanek et al, 2002). Although the coronary arteries may have anomalous origins, the vast majority of left and right coronary orifices are found at the left and right sinuses, respectively.…”

Section: Discussionmentioning

confidence: 99%

Role of VEGF family members and receptors in coronary vessel formation

Tomanek

Holifield

Reiter

et al. 2002

Developmental Dynamics

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The specific roles of vascular endothelial growth factor (VEGF) family members and their receptors (VEGFRs) in coronary vessel formation were studied. By using the quail heart explant model, we found that neutralizing antibodies to VEGF-B or VEGF-C inhibited tube formation on the collagen gel more than anti-VEGF-A. Soluble VEGFR-1, a receptor for VEGF-A and -B, inhibited tube formation by 87%, a finding consistent with that of VEGF-B inhibition. In contrast, addition of soluble VEGFR-2, a receptor for VEGF family members A, C, D, and E, inhibited tube formation by only 43%. Acidic FGF-induced tube formation dependency on VEGF was demonstrated by the attenuating effect of a soluble VEGFR-1 and -2 chimera. The localization of VEGF R-2 and R-3 was demonstrated by in situ hybridization of serial sections, which documented marked accumulations of transcripts for both receptors at the base of the truncus arteriosus coinciding with the temporal and spatial formation of the coronary arteries by means of ingrowth of capillary plexuses. This finding suggests that both VEGFR-2 and R-3 may play a role in the formation of the coronary artery roots. In summary, these experiments document a role for multiple members of the VEGF family and their receptors in formation of the coronary vascular bed.

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Section: Discussionmentioning

confidence: 99%

Role of VEGF family members and receptors in coronary vessel formation

Tomanek

Holifield

Reiter

et al. 2002

Developmental Dynamics

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“…For more detailed information regarding metabolic and mechanical influences on myocardial vascularization during postnatal development, see refs. [2,136].…”

Section: Arteriolar Formationmentioning

confidence: 97%

“…This process involves the assembly of endothelial cells into tubular structures (vasculogenesis) along a fibronectin network [61]. As seen by electron microscopy, some endothelial cells appear in blood islands, while others contain a large vacuole suggestive of intracellular lumen formation [2,61]. Blood islands are also the first sign of myocardial vascularization in mammals [55]; in humans they appear as early as Stage 14, i.e.…”

Section: Endothelial Cell Differentiation and Tubulogenesismentioning

confidence: 99%

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Formation of the coronary vasculature during development

Tomanek

2005

Angiogenesis

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107

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The formation of the coronary vasculature involves a series of carefully regulated temporal events that include vasculogenesis, angiogenesis, arteriogenesis and remodeling. This review explores these events, which begin with the migration of proepicardial cells to form the epicardium and end with postnatal growth and remodeling. Coronary endothelial, smooth muscle and fibroblast cells differentiate via epithelial-mesenchymal transformation; these cells delaminate from the epicardium. Following the formation of a tubular network by endothelial cells, an aortic ring of endothelial cells penetrates the aorta at the left and right aortic cusps to form the two ostia. Smooth muscle cell recruitment occurs rapidly and the coronary artery network begins forming as blood flow is established. Recent studies have identified a number of regulatory molecules that play key roles in epicardial formation and the transformation of its component cells into mesenchyme. Moreover, we are finally gaining some understanding regarding the interplay of angiogenic growth factors in the complex process of establishing the coronary vascular tree. Understanding coronary embryogenesis is important for interventions regarding adult cardiovascular diseases as well as those necessary to correct congenital defects.

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“…This process is followed by the formation of a vesicle that evolves into “comma” and “S” bodies, which will give rise to the glomerulus. As a result of this process, MM differentiates into nephrons, while the ureteric bud branches result in the collecting ducts [13–15]. …”

Section: Introductionmentioning

confidence: 99%

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

et al. 2017

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BackgroundKidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.ResultsIn this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.ConclusionOur results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.

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Vascular Development of the Heart

Cited by 6 publications

References 104 publications

Role of VEGF family members and receptors in coronary vessel formation

Role of VEGF family members and receptors in coronary vessel formation

Formation of the coronary vasculature during development

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

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