Vascular cell behavior on glycocalyx–mimetic surfaces: Simultaneous mimicking of the chemical composition and topographical structure of the vascular endothelial glycocalyx

Liang, Xiaofang; Zhang, Aiyang; Sun, Wenjuan; Jiao, Lei; Liu, Xiaoli; Tang, Zilong; Chen, Hong

doi:10.1016/j.colsurfb.2022.112337

Cited by 9 publications

(8 citation statements)

References 55 publications

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“…The introduction of the copolymer of sulfonate-containing monomer and glyco-containing monomer on the surface have also been shown to be conducive to HUVECs. 10,17 HUVECs were uniformly distributed on the SM−SM surface with a fusiform shape at an aspect ratio of ∼2.2.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…As shown in Figure 4a, HUVECs tended to adhere within the circles (PDMS area modified by pS) on S−M and adhere outside the circles (gold film area modified by pS) on M−S, indicating the good cell affinity of pS. 10,17 The HUVEC density on SM−SM was ∼285 cells/mm 2 , which was 2 and 1.7 times that of the S− M and M−S surfaces, respectively. The average spread area of HUVECs on SM−SM was ∼1479 μm 2 , which was 1.8 and 1.7 times that of S−M and M−S, respectively.…”

Section: ■ Experimental Methodsmentioning

confidence: 95%

“…14−16 It was found that glyco groups inhibited vascular cells, whereas sulfonate groups promoted vascular cell adhesion and proliferation whenever they were alone or combined with glyco groups. 10 When HMPs were uniformly distributed on the patterned surfaces, a highly complicated vascular cell behavior was observed. On chemically homogeneous surfaces, ordered micro/nano circular patterns did not interfere with the effect of HMPs on vascular cells, 10,17 topography obscured the effect of HMPs and showed a strong inhibition of vascular cells.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Previous studies have shown that HMP-modified surfaces exhibit similar biological functions to heparin-modified surfaces, including affecting vascular cell adhesion, proliferation, and differentiation. − Vascular cell behavior is a fundamental process among surface–blood interactions and provides a handle to optimize surface hemocompatibility . Ideal blood-contacting materials should selectively promote endothelial cell proliferation and concomitantly inhibit smooth muscle cell proliferation (“vascular cell selectivity”). − It was found that glyco groups inhibited vascular cells, whereas sulfonate groups promoted vascular cell adhesion and proliferation whenever they were alone or combined with glyco groups . When HMPs were uniformly distributed on the patterned surfaces, a highly complicated vascular cell behavior was observed.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Structural Elements of Heparin-Mimicking Polymers on Vascular Cell Distribution and Functions: Chemically Homogeneous or Heterogeneous?

Lei,

Sun,

Sheng

et al. 2023

ACS Biomater. Sci. Eng.

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Heparin-mimicking polymers (HMPs) are artificially synthesized alternatives to heparin with comparable regulatory effects on protein adsorption and cell behavior. By introducing two major structural elements of HMPs (sulfonate- and glyco-containing units) to different areas of material surfaces, heterogeneous surfaces patterned with different HMPs and homogeneous surfaces patterned with the same HMPs can be obtained. In this work, heterogeneous HMP-patterned poly(dimethylsiloxane) (PDMS) surfaces with sulfonate-containing polySS (pS) and glyco-containing polyMAG (pM) distributed in circular patterns (with a diameter of 300 μm) were prepared (S–M and M–S). Specifically, pS and pM were distributed inside and outside the circles on S–M, respectively, and exchanged their distribution on M–S. Homogeneous HMP-patterned silicone surfaces (SM–SM) where sulfonate- and glyco-containing poly(SS-co-MAG) (pSM) were distributed uniformly were prepared. Vascular cells showed interestingly different behaviors between chemically homogeneous and heterogeneous surfaces. They tended to grow in the sulfonate-modified area on S–M and M–S and were distributed uniformly on SM–SM. Compared with M–S, S–M showed a better promoting effect on the growth of vascular cells. Among all the samples, SM–SM exhibited the highest proliferation density and an optimum spreading state of vascular cells, as well as the highest human umbilical vein endothelial cell (HUVEC) viability (∼99%) and relatively low human umbilical vein smooth muscle cell (HUVSMC) viability (∼72%). By heterogeneous or homogeneous patterning with different structural elements of HMPs, the modified silicone surfaces spatially guided vascular cell distribution and functions. This strategy provides a new surface engineering approach to the study of cell–HMP interactions.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Experimental Methodsmentioning

confidence: 95%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Structural Elements of Heparin-Mimicking Polymers on Vascular Cell Distribution and Functions: Chemically Homogeneous or Heterogeneous?

Lei,

Sun,

Sheng

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

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“…There are also some experimental works dealing with chemical fabrication of glycocalyx-mimetic surfaces (see, e.g, [ 9 , 10 ]). Such artificial structures can be used for a direct investigation of glycocalyx-like substances and their interaction with blood flow.…”

Section: Introductionmentioning

confidence: 99%

Glycocalyx Sensing with a Mathematical Model of Acoustic Shear Wave Biosensor

Turova

Kovtanyuk

Pykhteev³

et al. 2022

Bioengineering

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The article deals with an idea of exploiting an acoustic shear wave biosensor for investigating the glycocalyx, a polysaccharide polymer molecule layer on the endothelium of blood vessels that, according to recent studies, plays an important role in protecting against diseases. To test this idea, a mathematical model of an acoustic shear wave sensor and corresponding software developed earlier for proteomic applications are used. In this case, the glycocalyx is treated as a layer homogenized over the thin polymer “villi”. Its material characteristics depend on the density, thickness, and length of the villi and on the viscous properties of the surrounding liquid (blood plasma). It is proved that the model used has a good sensitivity to the above parameters of the villi and blood plasma. Numerical experiments performed using real data collected retrospectively from premature infants show that the use of acoustic shear wave sensors may be a promising approach to investigate properties of glycocalyx-like structures and their role in prematurity.

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Hydrophilic cryogels as potential 3D cell culture materials: Synthesis and characterization of 2‐(methacrylamido) glucopyranose‐based polymer scaffolds

Behrendt,

Pretzel,

Cseresnyés

et al. 2023

Journal of Polymer Science

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Cell cultures are important techniques to investigate cellular processes in a simplified manner. The most commonly applied 2D models do not simulate most of the in vivo conditions whereas cryogels as promising 3D cell culture materials are able to provide both a structured microenvironment and tailored surface properties, similar to the natural extracellular matrix (ECM). Herein, we present the synthesis and characterization of hydrophilic cryogels with different 2‐(methacrylamido) glucopyranose amounts followed by 3D cell culture experiments with L929 cells. Selected samples are additionally functionalized with fibronectin‐FITC for improved cellular adhesion. The incorporation of different 2‐(methacrylamido) glucopyranose amounts into the cryogels is demonstrated by Fourier‐transform IR (FTIR) and solid‐state NMR (ssNMR). The gels are able to withstand harsh autoclaving conditions as shown by thermogravimetric analysis (TGA) and ssNMR measurements. Pore size measurements by automated scanning electron microscopy (SEM) image analysis reveal similar pore sizes among the entire cryogel series. Confocal laser scanning microscopy (CLSM) images suggest the presence of healthy cells due to the absence of morphological abnormalities among the entire cryogel series. L929 cells settled deeper into the cryogels with increasing MAG content. With additional fibronectin functionalization, the cells are retained much stronger and stay in the upper gel layers.

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Vascular cell behavior on glycocalyx–mimetic surfaces: Simultaneous mimicking of the chemical composition and topographical structure of the vascular endothelial glycocalyx

Cited by 9 publications

References 55 publications

Effect of Structural Elements of Heparin-Mimicking Polymers on Vascular Cell Distribution and Functions: Chemically Homogeneous or Heterogeneous?

Effect of Structural Elements of Heparin-Mimicking Polymers on Vascular Cell Distribution and Functions: Chemically Homogeneous or Heterogeneous?

Glycocalyx Sensing with a Mathematical Model of Acoustic Shear Wave Biosensor

Hydrophilic cryogels as potential 3D cell culture materials: Synthesis and characterization of 2‐(methacrylamido) glucopyranose‐based polymer scaffolds

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