Vascular augmentation of free adipose tissue grafts

Smahel, J; Meyer, Viktor E.; Schütz, Kathrin

doi:10.1007/bf00236371

Cited by 20 publications

(10 citation statements)

References 10 publications

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“…Transplants are largely absorbed and replaced by fibrous tissue and oil cysts [Peer, 1956] due to insufficient supply with nutrients and oxygen. Mature fat cells which do not get in contact with surrounding capillaries by inosculation within the first 4 days die [Smahel et al, 1990;von Heimburg and Pallua, 2001]. Any necrosis poses a risk to the surrounding tissue since infections are more likely to occur in necrotic tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the isolation of preadipocytes and their subsequent transplantation is superior to the transplantation of liposuctioned tissue or tissue grafts as a whole. In contrast to mature adipocytes, the transplantation of adipogenic precursor cells represents a highly promising source for soft tissue engineering [Smahel et al, 1990;von Heimburg et al, 2001bvon Heimburg et al, , 2001c since these cells are able to proliferate and differentiate into adipose tissue after reimplantation. In addition, preadipocytes survive a trauma of transplantation better than adipocytes, and they show good attachment and accumulation of fat at the new location [Billings and May, 1989].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to preadipocytes, adult adipocytes have lost the capacity to divide and are much more dependent on an adequate nutrient and oxygen supply [Smahel et al, 1990;von Heimburg and Pallua, 2001].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Viable Cell Yield from Excised versus Aspirated Adipose Tissue

Heimburg

Hemmrich

Haydarlioglu

et al. 2004

Cells Tissues Organs

128

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The correction of soft-tissue defects by adipose tissue transplantation often produces poor and unpredictable results. The implantation of isolated and cultured preadipocytes offers a solution to this problem since these cells differentiate into adipocytes when implanted in vivo. A field of major interest is to maximize the yield of preadipocytes isolated from adipose tissue showing only low contamination with other cell types. Aspiration and excision are two concurrent clinical ways of harvesting adipose tissue for the isolation of preadipocytes. This tissue is usually discarded after surgery. In this study, the yield of preadipocytes obtained from liposuction material was compared to that of excised adipose tissue. Furthermore, we determined the loss of precursor cells if isolation of preadipocytes was delayed for 24 h. Preadipocytes were isolated from the stromal cell fraction of human subcutaneous adipose tissue samples. Harvesting of adipose tissue by suction was performed according to the Coleman procedure (manually applied negative pressure using a 10-ml syringe with a blunt tip cannula). Isolation was either carried out within 60 min after extraction or after storage for 24 h in culture medium at 4°C. Isolated preadipocytes were cultured for 24 h, trypsinized and counted in a Neubauer chamber. Our results show clearly that the yield of preadipocytes isolated from liposuction material (within 60 min after extraction and after 24 h of storage) is higher than the cell yield from excised adipose tissue. Overnight storage for 24 h leads to a significant loss of preadipocytes in excised tissue but not in liposuction material. The high yield of cells isolated from liposuction material proves that extraction by suction does not damage the stromal cell fraction in the adipose tissue. If cell isolation is not performed immediately after the operation, liposuction material is clearly the better alternative for storage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparison of Viable Cell Yield from Excised versus Aspirated Adipose Tissue

Heimburg

Hemmrich

Haydarlioglu

et al. 2004

Cells Tissues Organs

128

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“…Moreover, in these conditions, the human 3-D singlelayer hypodermis presents the advantage of containing both preadipocytes, expressing pref-1, and mature adipocytes characterized by oil red O staining of lipids and by significant levels of adiponectin secretion [43] . The coexistence of preadipocytes and mature adipocytes may ensure the renewal of adipocytes in the hypodermis, knowing that mature adipocytes cannot survive for a long time in culture [4,5] .…”

Section: Discussionmentioning

confidence: 99%

“…Given that mature adipocytes do not proliferate, scientists have focussed on adipose-derived stem cells (ASC), their progenitors [4,5] , prepared for clinical pur-poses. Differentiated or not differentiated ASC of human [6][7][8] or animal origin [9,10] are often incorporated into scaffolds to facilitate their transfer [6,7,11,12] .…”

Section: Introductionmentioning

confidence: 99%

A Simple Way to Reconstruct a Human 3-D Hypodermis: A Useful Tool for Pharmacological Functionality

Lequeux

Auxenfans

Thépot

et al. 2011

Skin Pharmacol Physiol

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Background: Adipose tissue engineering has been hampered by the inability to culture mature adipocytes. Adipose-derived stem cell (ASC) culture opens the way for the preparation of human 3-D hypodermis in large quantities. These models play a role in obesity-related active molecules and slimming agent screening. Moreover, they contribute to a better understanding of the mechanisms underpinning obesity. Materials and Methods: Freshly extracted ASC from fat tissue were characterized by flow cytometry for CD73, CD90, CD105, HLA-ABC, CD14 and CD45 markers and by Western blot for pref-1. Their differentiation in mature adipocytes was followed by lipid and adiponectin secretion or by oil red O staining and radioimmunoassay. Neosynthesized extracellular matrix (ECM) of 3-D hypodermis was investigated by immunohistochemistry (collagen type I, V and VI) and transmission electron microscopy. Results: Our results demonstrate that the culture of preadipocytes in proliferation medium for 15 days followed by 16 days of culture in differentiation medium allowed production of the thickest single-layer hypodermis in which preadipocytes and mature adipocytes coexist and synthesize adiponectin and ECM components. Functionality of our 3-D single-layer hypodermis was demonstrated both by a 3.5-fold glycerol production after its stimulation with norepinephrine (adrenergic agonist) and by its slimming after caffeine treatment versus the nontreated 3-D hypodermis. Conclusion: This economic 3-D model, easy to prepare and giving reproducible results after the treatment of actives, is useful for pharmacotoxicological trials as an alternative to animal experimentation.

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Lipofilling und Liposkulptur

Pallua

Wolter

2016

Plastische Chirurgie

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Vascular augmentation of free adipose tissue grafts

Cited by 20 publications

References 10 publications

Comparison of Viable Cell Yield from Excised versus Aspirated Adipose Tissue

Comparison of Viable Cell Yield from Excised versus Aspirated Adipose Tissue

A Simple Way to Reconstruct a Human 3-D Hypodermis: A Useful Tool for Pharmacological Functionality

Lipofilling und Liposkulptur

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