Vascular and cellular responses to guanidino propyl piperazine CK0569A, a new anti‐plaque agent

Abstract

Hurttia H, Knuuttila M, Soderling E, Luostarinen V, Paunio K: V^ascular and cellular responses to guanidino propyl piperazine CK0569A, a new anti-plaque agent. Scand J Dent Res 1987; 95: 104-11.Abstract -Guanidino propyl piperazine GK0569A (abbreviation piperazine) used as a 0.1% mouthrinse bas been shown to be effective against plaque bacteria. In addition to its efficacy as an antibacterial agent, its safety sbould be taken into consideration. In this study the eifect of piperazine on the microcirctilation of the hamster cheek pouch was studied. The effect of this drug was also investigated on rat leukocytes in vivo and on macrophages and erythrocytes in vitro. Piperazine caused a distinct retardation in the microcirculation of tbe hamster cheeck pouch even at a low concentration (0.01%), but the effect was abolished in 5 min. The number of leukocytes decreased nsarkedly in the experimentally induced inflammatory exudate in the presence of 0.05% piperazine as compared to the controls. Tbe macrophages in vitro released their iysosomal and cytoplasmic enzymes and lost tbeir viability. The erythrocytes in vitro were destroyed in the presence of 0.005% piperazine as well. Based on these results it can be suggested that 0.005-0.05% piperazine under experimental conditions causes changes that indicate loss of cell viability.