Vascular and Cardiac Studies in
            <i>Drosophila</i>

Yu, Lin; Daniels, Joseph P.; Wolf, Matthew J.

doi:10.1002/9781118495148.ch50

Cited by 2 publications

(3 citation statements)

References 22 publications

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“…We also expressed green fluorescent protein (GFP) from the tinC enhancer ( tinC-GFP ) and OCT to visualize the beating heart in awake, adult flies. We measured the size of the ventricular diameter at the end of systole (ESD) and diastole (EDD) and calculated the fractional shortening, which is a measure of systolic function (30, 31). Compared to tinC-GFP; tinC-Gal4 flies, tinC-GFP; tinC-Gal4,UAS-yki S168A ( tinC>yki S168A ) flies had smaller EDDs and ESDs (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription

Daniels

et al. 2015

Sci. Signal.

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Organ hypertrophy can result from enlargement of individual cells or from cell proliferation or both. Activating mutations in the serine-threonine kinase Raf cause cardiac hypertrophy and contribute to Noonan syndrome in humans. Cardiac-specific expression of activated Raf also causes hypertrophy in Drosophila melanogaster. We found that Yorkie (Yki), a transcriptional coactivator in the Hippo pathway that regulates organ size, is required for Raf-induced cardiac hypertrophy in flies. Although aberrant activation of Yki orthologs stimulates cardiac hyperplasia in mice, cardiac-specific expression of an activated mutant form of Yki in fruit flies caused cardiac hypertrophy without hyperplasia. Knockdown of Yki caused cardiac dilation without loss of cardiomyocytes and prevented Raf-induced cardiac hypertrophy. In flies, Yki-induced cardiac hypertrophy required the TEA domain–containing transcription factor Scalloped, and, in mammalian cells, expression of mouse RafL613V, an activated form of Raf with a Noonan syndrome mutation, increased Yki-induced Scalloped activity. Furthermore, overexpression of Tgi (a Tondu domain–containing Scalloped-binding corepressor) in the fly heart abrogated Yki- or Raf-induced cardiac hypertrophy. Thus, crosstalk between Raf and Yki occurs in the heart and can influence Raf-mediated cardiac hypertrophy.

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Section: Resultsmentioning

confidence: 99%

“…Fly heart wall thicknesses were measured as previously described (31, 53). Briefly, adult female flies of 2 to 7 days after eclosion were collected and fixed in Telly’s fixation buffer (60% ethanol, 3.33% formalin, 4% glacial acetic acid) for at least 1 week at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription

Daniels

et al. 2015

Sci. Signal.

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“…The heart tube consists of a single layer of cardiomyocytes and performs pulsatile contractions, which result in hemolymph flow [ 6 ]. Given the high conservation of genes and proteins found in Drosophila in the mammalian heart, it can be used to investigate pathways and mechanisms influencing heart function in mammals [ 7 , 8 ]. The liver equivalent is called the fat body and is important for maintaining energy storage, immune response and nutrient sensing [ 9 ].…”

Section: Drosophila Melanogaster As a Model Organismmentioning

confidence: 99%

Insights into human kidney function from the study of Drosophila

Koehler

Huber

2023

Pediatr Nephrol

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Biological and biomedical research using Drosophila melanogaster as a model organism has gained recognition through several Nobel prizes within the last 100 years. Drosophila exhibits several advantages when compared to other in vivo models such as mice and rats, as its life cycle is very short, animal maintenance is easy and inexpensive and a huge variety of transgenic strains and tools are publicly available. Moreover, more than 70% of human disease-causing genes are highly conserved in the fruit fly. Here, we explain the use of Drosophila in nephrology research and describe two kidney tissues, Malpighian tubules and the nephrocytes. The latter are the homologous cells to mammalian glomerular podocytes and helped to provide insights into a variety of signaling pathways due to the high morphological similarities and the conserved molecular make-up between nephrocytes and podocytes. In recent years, nephrocytes have also been used to study inter-organ communication as links between nephrocytes and the heart, the immune system and the muscles have been described. In addition, other tissues such as the eye and the reproductive system can be used to study the functional role of proteins being part of the kidney filtration barrier.

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Vascular and Cardiac Studies in Drosophila

Cited by 2 publications

References 22 publications

Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription

Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription

Insights into human kidney function from the study of Drosophila

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