Vasa, a regulator of localized mRNA translation on the spindle

Buitrago, Paola Alejandra Sundaram; Rao, Kavya; Yajima, Mamiko

doi:10.1002/bies.202300004

Cited by 3 publications

(2 citation statements)

References 111 publications

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“…BASP1, is a peptide which probably is involved in fertilization-induced oocyte activation (Zakharova and & Zakharov, 2017) while the immunoglobulins (IGHG1) have been suggested to counteract the increased ROS levels and assist the oocyte to survive in adverse environments (Wang et al ., 2022). Additionally, DDX4, the human ortholog of VASA, is a well-known germ cell marker that has also been suggested to be involved in the regulation of translation (Castrillon et al ., 2000; Sundaram et al ., 2023). The roles of IGHG1, BASP1, and DDX4 in oocyte function and translation regulation suggest that they may be critical factors in maintaining oocyte quality.…”

Section: Discussionmentioning

confidence: 99%

Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

Galatidou,

Petelski,

Pujol

et al. 2024

Preprint

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Advanced maternal age is associated with a decline in oocyte quality, which often leads to reproductive failure in humans. However, the mechanisms behind this age-related decline remain unclear. To gain insights into this phenomenon, we applied plexDIA, a multiplexed, single-cell mass spectrometry method, to analyze the proteome of oocytes from both young women and women of advanced maternal age. Our findings primarily revealed distinct proteomic profiles between immature fully grown germinal vesicle and mature metaphase II oocytes. Importantly, we further show that a woman's age is associated with changes in her oocyte proteome. Specifically, when compared to oocytes obtained from young women, advanced maternal age oocytes exhibited lower levels of the proteasome and TRiC complex, as well as other key regulators of proteostasis and meiosis. This suggests that aging adversely affects the proteostasis and meiosis networks in human oocytes. The proteins identified in this study hold potential as targets for improving oocyte quality and may guide future studies into the molecular processes underlying oocyte aging.

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Section: Discussionmentioning

confidence: 99%

Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

Galatidou,

Petelski,

Pujol

et al. 2024

Preprint

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“…The gene vasa encodes an ATP-dependent RNA helicase of the DEAD (Asp-Glu-Ala-Asp)-box family protein, initially discovered in Drosophila as a maternal-effect gene [ 1 ]. Subsequently, vasa homolog genes have been identified in various species including mice ( Mus spretus ) [ 2 ], Xenopus [ 3 ], silkworms ( Bombyx mori ) [ 4 ], humans ( Homo sapiens ) [ 5 ], and the sea urchin ( Anthocidaris crassispina ) [ 6 ]. In fish, the vasa gene was first characterized in zebrafish ( Danio rerio ) [ 7 , 8 ], followed by medaka ( Oryzias latipes ) [ 9 , 10 ], tilapia ( Oreochromis niloticus ) [ 11 ], gibel carp ( Carassius auratus gibelio ) [ 12 ], southern catfish ( S. meridionalis ) [ 13 ], discus fish ( Symphysodon haraldi ) [ 14 ], black carp ( Mylopharyngodon Piceus ) [ 15 ], and so on.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the Vasa Gene in Silurus asotus and Its Expression Response to Letrozole Treatment

Yu,

Wang,

et al. 2024

Genes

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The identification and expression of germ cells are important for studying sex-related mechanisms in fish. The vasa gene, encoding an ATP-dependent RNA helicase, is recognized as a molecular marker of germ cells and plays a crucial role in germ cell development. Silurus asotus, an important freshwater economic fish species in China, shows significant sex dimorphism with the female growing faster than the male. However, the molecular mechanisms underlying these sex differences especially involving in the vasa gene in this fish remain poorly understood. In this work, the vasa gene sequence of S. asotus (named as Savasa) was obtained through RT-PCR and rapid amplification of cDNA end (RACE), and its expression in embryos and tissues was analyzed using qRT-PCR and an in situ hybridization method. Letrozole (LT) treatment on the larvae fish was also conducted to investigate its influence on the gene. The results revealed that the open reading frame (ORF) of Savasa was 1989 bp, encoding 662 amino acids. The SaVasa protein contains 10 conserved domains unique to the DEAD-box protein family, showing the highest sequence identity of 95.92% with that of Silurus meridionalis. In embryos, Savasa is highly expressed from the two-cell stage to the blastula stage in early embryos, with a gradually decreasing trend from the gastrula stage to the heart-beating stage. Furthermore, Savasa was initially detected at the end of the cleavage furrow during the two-cell stage, later condensing into four symmetrical cell clusters with embryonic development. At the gastrula stage, Savasa-positive cells increased and began to migrate towards the dorsal side of the embryo. In tissues, Savasa is predominantly expressed in the ovaries, with almost no or lower expression in other detected tissues. Moreover, Savasa was expressed in phase I–V oocytes in the ovaries, as well as in spermatogonia and spermatocytes in the testis, implying a specific expression pattern of germ cells. In addition, LT significantly upregulated the expression of Savasa in a concentration-dependent manner during the key gonadal differentiation period of the fish. Notably, at 120 dph after LT treatment, Savasa expression was the lowest in the testis and ovary of the high concentration group. Collectively, findings from gene structure, protein sequence, phylogenetic analysis, RNA expression patterns, and response to LT suggest that Savasa is maternally inherited with conserved features, serving as a potential marker gene for germ cells in S.asotus, and might participate in LT-induced early embryonic development and gonadal development processes of the fish. This would provide a basis for further research on the application of germ cell markers and the molecular mechanisms of sex differences in S. asotus.

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Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

Galatidou,

Petelski,

Pujol

et al. 2024

Molecular Human Reproduction

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Advanced maternal age is associated with a decline in oocyte quality, which often leads to reproductive failure in humans. However, the mechanisms behind this age-related decline remain unclear. To gain insights into this phenomenon, we applied plexDIA, a multiplexed data-independent acquisition, single-cell mass spectrometry method, to analyze the proteome of oocytes from both young women and women of advanced maternal age. Our findings primarily revealed distinct proteomic profiles between immature fully grown germinal vesicle and mature metaphase II oocytes. Importantly, we further show that a woman’s age is associated with changes in her oocyte proteome. Specifically, when compared to oocytes obtained from young women, advanced maternal age oocytes exhibited lower levels of the proteasome and TRiC complex, as well as other key regulators of proteostasis and meiosis. This suggests that aging adversely affects the proteostasis and meiosis networks in human oocytes. The proteins identified in this study hold potential as targets for improving oocyte quality and may guide future studies into the molecular processes underlying oocyte aging.

show abstract

Vasa, a regulator of localized mRNA translation on the spindle

Cited by 3 publications

References 111 publications

Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

Characteristics of the Vasa Gene in Silurus asotus and Its Expression Response to Letrozole Treatment

Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

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