Various stages of Schistosoma express Lewisx, LacdiNAc, GalNAc 1-4 (Fuc 1-3)GlcNAc and GalNAc 1-4(Fuc 1-2Fuc 1-3)GlcNAc carbohydrate epitopes: detection with monoclonal antibodies that are characterized by enzymatically synthesized neoglycoproteins

Remoortere, Alexandra van; Hokke, Cornelis H.; Dam, Govert J. van; Die, Irma van; Deelder, André M.; Eijnden, Dirk H. van den

doi:10.1093/glycob/10.6.601

Cited by 122 publications

(131 citation statements)

References 38 publications

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“…Other synthetic immunogenic schistosome oligosaccharides including Lewis X, GalNAcb1 fi 4GlcNAc and GalNAcb1 fi 4(Fuca1 fi 2Fuca1 fi 3)GlcNAc were successfully applied to monitor antibody responses in S. mansoni, S. japonicum, or S. haematobium-infected patients (Van Remoortere et al 2001a). In addition, these oligosaccharides allowed the characterisation of schistosome-specific mAbs similar to the current study, and it appeared that generally both IgM and IgG type mAbs specific for a particular oligosaccharide epitope occurred (Van Remoortere et al 2000).…”

Section: Discussionsupporting

confidence: 65%

“…The Sensor Chip CM5 and Amine Coupling Kit were also obtained from BIAcore. The neoglycoconjugates were immobilised at a flow rate of 5 ll/min in 10 mM sodium acetate (pH 4.0) onto a carboxylmethylated dextran CM5 sensor chip by covalent amine coupling until an increase of approximately 3,500-4,500 response units (RU) was observed using described procedures (Van Remoortere et al 2000, 2001a. On each CM5 sensor chip there was also a control BSA channel prepared to correct for the buffer-related RU change on injection, matrix effects and non-specific interactions (Myszka 1999).…”

Section: Inhibition Elisamentioning

confidence: 99%

“…To test the potential of the synthetic oligosaccharides for the detection of serum antibodies, sera obtained from schistosome-infected individuals (n=67) and healthy controls (n=5) were subjected to BIAcore determination of specific IgM and IgG levels according to the previously reported methods (Van Remoortere et al 2000, 2001a. The infection sera were studied by IFA and ELISA to determine the presence of IgM against S. mansoni male adult worms and of IgG against S. mansoni soluble egg antigens, respectively, and subsequently categorised into four groups according to the antibody titers (Nash 1974;Deelder et al 1980).…”

Section: Immunoreactivity Of Infection Sera With the Glycoconjugatesmentioning

confidence: 99%

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Immunodiagnostically applicable monoclonal antibodies to the circulating anodic antigen of Schistosoma mansoni bind to small, defined oligosaccharide epitopes

et al. 2003

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Gut-associated glycoproteins constitute a major group of the circulating excretory antigens produced by human Schistosoma species. The O-glycans of the relatively abundant circulating anodic antigen (CAA) from S. mansoni carry long stretches of unique fi 6(Glc Ab1 fi 3)GalNAcb1 fi repeats. Specific anti-carbohydrate monoclonal antibodies (mAbs) are essential tools for the immunodiagnostic detection of CAA in the serum or urine of Schistosoma-infected subjects. In order to define the epitopes recognised by these anti-CAA mAbs, we screened a series of protein-coupled synthetic di-to pentasaccharide building blocks of the CAA polysaccharide for immunoreactivity, using ELISA and surface plasmon resonance spectroscopy. It was shown that anti-CAA IgM mAbs preferentially recognise fi 6(GlcAb1 fi 3)GalNAcb1 fi disaccharide units. Interestingly, no mouse anti-CAA mAbs of the IgG class were found that bind to the synthetic epitopes, although many of the IgG mAbs tested do recognise native CAA in a carbohydratedependent manner. In addition, both IgM and IgG class antibodies could be detected in human infection sera using the synthetic CAA fragments. These synthetic schistosome glycan epitopes and their matching set of specific mAbs are useful tools that further the development of diagnostic methods and are helpful in defining the immunological responses of the mammalian hosts to schistosome glycoconjugates.

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Section: Discussionsupporting

confidence: 65%

Section: Inhibition Elisamentioning

confidence: 99%

Section: Immunoreactivity Of Infection Sera With the Glycoconjugatesmentioning

confidence: 99%

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Immunodiagnostically applicable monoclonal antibodies to the circulating anodic antigen of Schistosoma mansoni bind to small, defined oligosaccharide epitopes

et al. 2003

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“…The correct presentation of Lewis X epitopes at the surface was confirmed by monoclonal antibodies (291-4D10-A and 22-1B3-A; ref. 19) directed against Lewis X.…”

Section: Preparation Of Neoglycoconjugates 4 Andmentioning

confidence: 99%

Carbohydrate self-recognition mediates marine sponge cellular adhesion

Haseley

Vermeer

Kamerling

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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Sponges (Porifera), the simplest and earliest multicellular organisms, are thought to have evolved from their unicellular ancestors about 1 billion years ago by developing cell-recognition and adhesion mechanisms to discriminate against ''non-self.'' Consequently, they are used as models for investigating recognition phenomena. Cellular adhesion of marine sponges is an event involving adherence of extracellular proteoglycan-like molecules, otherwise known as aggregation factors (AFs). In a calciumindependent process the AFs adhere to the cell surface, and in a calcium-dependent process they exhibit AF self-association. A mechanism which has been implied but not definitely proven to play a role in the calcium-dependent event is self-recognition of defined carbohydrate epitopes. For the red beard sponge, Microciona prolifera, two carbohydrate epitopes, a sulfated disaccharide and a pyruvylated trisaccharide, have been implicated in cellular adhesion. To investigate this phenomenon a system has been designed, by using surface plasmon resonance detection, to mimic the role of carbohydrates in cellular adhesion of M. prolifera. The results show self-recognition of the sulfated disaccharide to be a major force behind the calcium-dependent event. The interaction is not simply based on electrostatic interactions, as other sulfated carbohydrates analyzed by using this procedure did not selfassociate. Furthermore, the interaction is completely eradicated on substitution of Ca 2؉ ions by either Mg 2؉ or Mn 2؉ ions. This physiologically relevant recognition mechanism confirms the existence of true carbohydrate self-recognition, and may have significant implications for the role of carbohydrates in cellular recognition of higher organisms. W eak polyvalent interactions play an important role in biological processes. There is growing evidence that carbohydrates, found on the surfaces of all living cells, are functional constituents in cell-cell interactions. At present, only a few examples of low-affinity carbohydrate-carbohydrate interactions are known (1-5). For example, pioneering work by Hakomori and his colleagues (1-3) has shown glycosphingolipid self-interaction to occur by way of multivalent interaction of Lewis X epitopes.Since 1900, marine sponges have been used as primitive models for studying the phenomenon of cell recognition. Knowledge of the recognition mechanisms of these simple organisms, hypothetically situated at the foot of the metazoan kingdom, may contribute to the understanding of cell-cell adhesion events within higher organisms. Adhesion of marine sponges is an event that involves both calcium-independent adherence of proteoglycan-like molecules, named aggregation factors (AFs), to the cell surface, and calcium-dependent AF self-association (6-8). The calcium-dependent event is species-specific, as illustrated by the rapid self-association and sorting, on the addition of calcium ions, of a mixture of colored (pink, yellow, and white) proteoglycan-coated beads, each color corresponding to a different s...

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“…The lectin HPA (Helix pomatia agglutinin) was purchased from Sigma-Aldrich. An anti-LDN (IgG, clone Y1H5), an anti-LDNF (IgG, clone L6B8), an anti-Le x (IgM, clone 5F1), two anti-LDN-dF (IgM, clone 290-2D9-A; IgM, clone 290-4A8), and an anti-Man 3 GlcNAc 2 (M3GN2; IgM, clone 100-4G11-A) MAb, as well as an anti-FLNDF antibody (IgG, clone F2D2), were developed as MAbs by the production of hybridomas from spleens of mice that had been infected with S. mansoni by using methods described previously (16,(39)(40)(41)(42). The anti-horseradish peroxidase (HRP) antibody (anti-peroxidase, polyclonal rabbit IgG; P7899) was purchased from Sigma-Aldrich and used to detect core ␣3 fucose and core xylose (CF/CX) determinants (43,44).…”

Section: Methodsmentioning

confidence: 99%

Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray

et al. 2016

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f Infection of mammals by the parasitic helminth Schistosoma mansoni induces antibodies to glycan antigens in worms and eggs, but the differential nature of the immune response among infected mammals is poorly understood. To better define these responses, we used a shotgun glycomics approach in which N-glycans from schistosome egg glycoproteins were prepared, derivatized, separated, and used to generate an egg shotgun glycan microarray. This array was interrogated with sera from infected mice, rhesus monkeys, and humans and with glycan-binding proteins and antibodies to gather information about the structures of antigenic glycans, which also were analyzed by mass spectrometry. A major glycan antigen targeted by IgG from different infected species is the FLDNF epitope [Fuc␣3GalNAc␤4(Fuc␣3)GlcNAc-R], which is also recognized by the IgG monoclonal antibody F2D2. The FLDNF antigen is expressed by all life stages of the parasite in mammalian hosts, and F2D2 can kill schistosomula in vitro in a complement-dependent manner. Different antisera also recognized other glycan determinants, including core ␤-xylose and highly fucosylated glycans. Thus, the natural shotgun glycan microarray of schistosome eggs is useful in identifying antigenic glycans and in developing new anti-glycan reagents that may have diagnostic applications and contribute to developing new vaccines against schistosomiasis. Schistosomiasis is a major health problem in tropical and subtropical areas where it is endemic, with more than 200 million people actively infected and 800 million at risk of contracting the disease (1-3). Current treatment for disease is limited to the drug praziquantel (4), but cases of drug resistance have been reported (5). Decades of research on schistosomiasis vaccines have yielded only two candidates for clinical trials, and no encouraging results have been published yet (6-9). Thus, there is an urgent need to develop more sensitive diagnostic methods and to identify new vaccine candidates.Recent studies have shown that a major part of the host immune response to infection is directed against carbohydrate (glycan) antigens in glycoproteins and glycolipids (10-17). A wide variety of unusual antigenic determinants include glycans containing the LDN, fucosylated LDN sequences (LDNF, LDN-dF, FLDN, and FLDNF), Lewis X (Le x ), poly-Le x , core ␣3 fucose, and core ␤2 xylose structures (Fig. 1) (11, 14, 17), many of which are expressed by all developmental stages of schistosomes (18). Interestingly, monoclonal antibodies (MAbs) specific to these glycans recognize these antigens on the surface of 3-h-old schistosomula, and some anti-glycan antibodies can mediate killing in vitro in a complement-dependent fashion (18-21). Schistosoma mansoniinfected rhesus monkeys, which are known to self-cure after infection, have IgG to many glycan antigens, including Le x , LDN, LDNF, core fucose, and core xylose determinants, and their sera are effective in complement-mediated cytolysis of cells expressing Le x as well as schistosomulum larvae in ...

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Various stages of Schistosoma express Lewisx, LacdiNAc, GalNAc 1-4 (Fuc 1-3)GlcNAc and GalNAc 1-4(Fuc 1-2Fuc 1-3)GlcNAc carbohydrate epitopes: detection with monoclonal antibodies that are characterized by enzymatically synthesized neoglycoproteins

Cited by 122 publications

References 38 publications

Immunodiagnostically applicable monoclonal antibodies to the circulating anodic antigen of Schistosoma mansoni bind to small, defined oligosaccharide epitopes

Immunodiagnostically applicable monoclonal antibodies to the circulating anodic antigen of Schistosoma mansoni bind to small, defined oligosaccharide epitopes

Carbohydrate self-recognition mediates marine sponge cellular adhesion

Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray

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