Various methods available for detection of apoptotic cells- A review

Archana, M; Bastian,; Yogesh, T L; Kumaraswamy, K L

doi:10.4103/0019-509x.118720

Cited by 70 publications

(24 citation statements)

References 58 publications

(64 reference statements)

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“…This is an inexpensive method for the detection of apoptotic cells. Nevertheless, it lacks objectivity, reproducibility, and sensitivity and it is prone to more errors [ 186 ].…”

Section: In Vitro Angiogenesis Assaysmentioning

confidence: 99%

A Critical Analysis of the AvailableIn VitroandEx VivoMethods to Study Retinal Angiogenesis

Moleiro

Conceição

Leite‐Moreira

et al. 2017

Journal of Ophthalmology

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Angiogenesis is a biological process with a central role in retinal diseases. The choice of the ideal method to study angiogenesis, particularly in the retina, remains a problem. Angiogenesis can be assessed through in vitro and in vivo studies. In spite of inherent limitations, in vitro studies are faster, easier to perform and quantify, and typically less expensive and allow the study of isolated angiogenesis steps. We performed a systematic review of PubMed searching for original articles that applied in vitro or ex vivo angiogenic retinal assays until May 2017, presenting the available assays and discussing their applicability, advantages, and disadvantages. Most of the studies evaluated migration, proliferation, and tube formation of endothelial cells in response to inhibitory or stimulatory compounds. Other aspects of angiogenesis were studied by assessing cell permeability, adhesion, or apoptosis, as well as by implementing organotypic models of the retina. Emphasis is placed on how the methods are applied and how they can contribute to retinal angiogenesis comprehension. We also discuss how to choose the best cell culture to implement these methods. When applied together, in vitro and ex vivo studies constitute a powerful tool to improve retinal angiogenesis knowledge. This review provides support for researchers to better select the most suitable protocols in this field.

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“…This is an inexpensive method for the detection of apoptotic cells. Nevertheless, it lacks objectivity, reproducibility, and sensitivity and it is prone to more errors [ 186 ].…”

Section: In Vitro Angiogenesis Assaysmentioning

confidence: 99%

A Critical Analysis of the AvailableIn VitroandEx VivoMethods to Study Retinal Angiogenesis

Moleiro

Conceição

Leite‐Moreira

et al. 2017

Journal of Ophthalmology

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“…We can assume that in the remote post-mortem period, the processes of myocardial rehabilitation are carried out by increasing the synthesis of cellular material against the background of amplification of apoptosis of damaged cells. Despite the presence of views on the protective role of apoptosis after thermal damage, comparing the clinical data of other researchers and the results obtained by us suggests that heart damage can occur precisely on the background of amplification of apoptosis [4,20,21]. This may also indicate an increase in the S-phase, set 7 days after the thermal trauma, which, in turn, indicates a lack of cardiomyocyte reparation processes in the early stages of the burn injury.…”

Section: Discussionmentioning

confidence: 51%

Indicators of the Cardiomyocytes` Cells Cycle Under Infusion of Blood Substitutes and in the Correction of Experimental Burn Injury by 0,9% Nacl Solution

Radoga

2018

Rep. of Morph.

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According to the WHO, the thermal trauma is on the third place among other injuries. Burned injury is not only damage to the skin, but also the traumatization of all organs and systems of the body as a result of the stress response of the vascular system and the effects of toxic products coming from the area of burn injury. Firstly, such damages affect cardiomyocytes and the microcirculation vessels of the heart. The purpose of our study was to evaluate the changes in the cell cycle of myocardial cells in the left ventricle of rats under conditions of blood substitutes infusion and in the correction of experimental burn injury with a 0,9% solution of NaCl. The burn trauma was modeled using the Regas’ method and placed a catheter into the lower vena cava for intravenous infusion. The following solutions were used for infusion: 0,9% NaCl solution, lactoproteinum with sorbitol (Lactoproteinum-C) and colloidal-hyperosmolar HAES-LX-5% solution. Flow cytometry of the nuclear suspension of left ventricular cardiomyocytes was performed on the 1st, 3rd, and 7th days of the experiment. The statistical analysis of the results was carried out using the “STATISTICA 6.1” program package. The results of the performed study show a fairly stable picture of cell cycle parameters in myocardial cells of animals without burn injury with a predominance, on the one hand, of cells present in the G0G1 phase and the presence of a certain balance between the processes of creation of nuclear DNA synthesis and apoptosis. Changes in the phase of cardiac myocyte cell cycle against the background of the thermal injury of the skin throughout the observation time indicate a prolonged, uncorrected cell cycle disorder and a lack of effective normalization on the background of the physiological solution usage in the first 7 days after burning trauma of the skin. The protective effect of HAES-LX-5% prevents over-strain of cells, as evidenced by the lower synthetic activity of nuclei of cardiomyocytes at all times of the experiment.

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“…Apoptotic bodies were found with hematoxylin-eosin staining in most rGST-P-positive areas (21). During regression of mitogen-induced hyperplasia, non-proliferating (aged) hepatocytes seemed to be preferred for apoptosis.…”

Section: Discussionmentioning

confidence: 91%

Hepatic preneoplasia induction in male Wistar rats: histological studies up to five months post treatment

2016

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Background: Liver preneoplasia development in rats can be mimicked by an initiation-promotion model that induces the appearance of altered hepatocyte foci (FAH).Aims: We compare two initiation-promotion models to evaluate the presence of FAH or additional hepatic pathologies in which other organs were affected up to five month post treatment.Material and methods: FAH were induced in male adult Wistar rats with two doses of dietylnitrosamine (DEN, 150 mg/kg bw) followed by 4 doses per week (3 weeks) of 2-acetylaminofluorene (2-AAF, 20 mg/kg bw) or with one dose of DEN (200 mg/kg bw) followed by 2 doses per week (3 weeks) of 2-AAF. DEN 150, DEN 200 and control rats (received the vehicle of the drugs) groups were compared. Rats were euthanized immediately after the last dose of 2-AAF, at 3, 4 and 5 months (n = 3 for euthanasia times per group). Samples of livers, lungs, kidneys, pancreatic tissue and small bowel were processed for histological and immunohistochemical analysis.Results: FAH persisted for 5 months in all livers of the DEN groups. Three months after withdrawal of 2-AAF, one rat from DEN 150 group developed fibrosis and 5 months after 2-AAF removal another rat from the same group presented a microscopic hyperplastic nodule. Only the lungs had damages compatible with lesions induced by gavage-related reflux in DEN groups.Conclusion: We concluded that up to five month post treatments, FAH persisted in all the livers from DEN groups; livers from DEN 200 group showed no other hepatic lesions besides FAH, and only the lungs suffered pathological alterations in both treated groups.

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Various methods available for detection of apoptotic cells- A review

Cited by 70 publications

References 58 publications

A Critical Analysis of the AvailableIn VitroandEx VivoMethods to Study Retinal Angiogenesis

A Critical Analysis of the AvailableIn VitroandEx VivoMethods to Study Retinal Angiogenesis

Indicators of the Cardiomyocytes` Cells Cycle Under Infusion of Blood Substitutes and in the Correction of Experimental Burn Injury by 0,9% Nacl Solution

Hepatic preneoplasia induction in male Wistar rats: histological studies up to five months post treatment

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