Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix

In this study, pyrrole/N‐para‐toluenpyrrole (Py/NptPy) copolymers were synthesized by chemical oxidative polymerization method. The effects of different kinds of (anionic, cationic, and nonionic) surfactants on the properties of copolymer were investigated. The structural, morphological, thermal, and conductivity properties of the copolymers were investigated by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and the four‐probe measurement device respectively. FTIR spectra of copolymers synthesized in different environments that support the formation of copolymer. SEM results show that the morphologies of copolymers synthesized in the presence of surfactant are more homogeneous and the particle sizes are smaller than that of polypyrrole (PPy). TGA results revealed that the thermal stabilities of Py/NptPy‐NaDBS (400°C) and Py/NptPy‐Tween 20 (260°C) copolymers were higher than surfactant free Py/NptPy copolymer (180°C) according to their initial decomposition temperatures. It was determined that the conductivity values of copolymers were higher than PNptPy. Glucose sensor properties of copolymer synthesized in the presence of tetradecylthreemethyl ammonium bromide (TTAB) were investigated with cyclic voltammetry (CV) method. Michaelis–Menten constant of Py/NptPy‐TTAB copolymer modified electrochemical biosensor was calculated as 5.30 mM. POLYM. ENG. SCI. 56:995–1003, 2016. © 2016 Society of Plastics Engineers

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“…a) has a globular and cauliflower‐like morphology compared to PNptPy (Fig. b) . PNptPy has more flat and “leafy” morphology.…”

Section: Resultsmentioning

confidence: 98%

Pyrrole/N‐p‐toluenepyrrole copolymers in the presence of surfactants: A promising material for glucose sensing

Ozkan

Gürsoy

2016

Polymer Engineering & Sci

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“…As can be seen from the Table 1, the K m is much lower than those reported for Copolymers of vinyl alcohol with thiophene side groups and pyrrole/GOx, 47 Poly(2-Dodecyl-4,7-di(thiophen-2-yl)-2H-benzo[d] [1,2,3]triazole)/GOx, 31 Poly (N-(4-(3-thienylmethylene)-oxycarboyl phenyl) maleimideco-pyrrole/GOx. 41 The integration of biosensors to FIA mode has a large variety of applications (37,38). The advantage of FIA devices is their possible uses in multiple measurements simultaneously (39).…”

Section: Analytical Characteristicsmentioning

confidence: 99%

Oligomeric Thiosemicarbazones as Novel Immobilization Matrix in Biosensing Applications

Seven

Demirdoven

Yıldırım

et al. 2013

Journal of Macromolecular Science, Part A

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Thiosemicarbazone (TSC) matrices are constructed by electrochemically oligomerization on the graphite electrodes to investigate their matrix properties in terms of biomolecule immobilization for the biosensor applications. Amino functionalized oligomeric TSCs were successfully applied for the glucose oxidase (GOx) immobilization using glutaraldehyde (GA) as the crosslinker. Scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FT-IR) measurements are used to characterize the modified surface. Electrochemical measurements of the GOx biosensor were performed at ambient conditions at −0.7 vs. Ag/AgCl electrode by following the oxygen consumption resulting from the enzyme activity, in the presence of glucose substrate. Linearity, operational stabilities and repeatability are investigated in detail, as well as optimization of pH and enzyme amount,. The optimized biosensor shows a very good linearity between 0.05 mM and 1.0 mM glucose with a 10 s response time. Kinetic parameters such as K m and I max are also calculated. Besides the batch configuration, the analytical characteristics of the system were also evaluated in flow injection analysis (FIA) mode. Finally, the new biosensor configuration was applied for glucose determination on a real sample.

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“…A preliminary study showed that HRP immobilized onto polyaniline was very stable for hydrogen peroxide determination (FERNANDES et al, 2005). Several authors reported stability of enzyme biosensors containing immobilized GOD (ÇIL et al, 2007;MISLOVICOVÁ;MICHÁLKOVÁ;VIKARTOVSKÁ, 2007;YILDIZ et al, 2005;FERREIRA;FIORITO;OLIVEIRA JUNIOR, 2004;GÜRSEL et al, 2003). The variation observed in this parameter is very high, and as such it is possible to find stabilities from 45% of initial activity after 5 days of use and storage to 90% of initial activity after 45 cycles of use.…”

Section: Long-term Stabilities Of God/hrp Biosensormentioning

confidence: 99%

“…1.11.1.7) in a sequence of redox reactions. Glucose oxidase as a free enzyme has been used in the measurement of glucose in foods and beverages (ÇIL et al, 2007;WILSON;TURNER, 1992;SARTINI et al, 1998) even though it represents an important cost component of a product (WU et al, 2004). To overcome this inconvenience, GOD has been immobilized on various supports with the purpose of its economic reuse (MISLOVICOVÁ;MICHÁLKOVÁ;VIKARTOVSKÁ, 2007;ENDO et al, 2006;FERREIRA;FIORITO;OLIVEIRA JUNIOR, 2004;GÜRSEL et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Biosensor for determination of glucose in real samples of beverages

Lopes

Batista

et al. 2012

Food Sci. Technol

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A biosensor was developed for spectrophotometric determination of glucose concentrations in real samples of orange juice energetic drinks, and sport drinks. The biosensor consisted of glucose oxidase (GOD) and horseradish peroxidase (HRP) immobilized onto polyaniline activated with glutaraldehyde (PANIG). Immobilization parameters were optimized for GOD, and maximum immobilization yield was 16% when 5.0 mg of PANIG and 8.9 U prepared in 0.1 mol.L-1 sodium phosphate buffer (pH 7.0) reacted for 60 minutes at 4 °C with gentle stirring. The linear operational range for glucose determination using optimized operational parameters was between 0.05 and 6.0 mg.mL-1 with a very good reproducibility of response. The results obtained in the biosensor were compared with those obtained using free enzymes (commercial kits) and then validated through statistical analysis using the Tukey test (95% confidence interval).

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Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix

Cited by 25 publications

References 16 publications

Pyrrole/N‐p‐toluenepyrrole copolymers in the presence of surfactants: A promising material for glucose sensing

Pyrrole/N‐p‐toluenepyrrole copolymers in the presence of surfactants: A promising material for glucose sensing

Oligomeric Thiosemicarbazones as Novel Immobilization Matrix in Biosensing Applications

Biosensor for determination of glucose in real samples of beverages

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