Varicocele, leucocitospermia y su impacto en la fragmentación del ADN espermático

Rodríguez, Beatriz L.; López‐Fernández, Carmen; Núñez-Calonge, Rocío; Caballero, Pedro; Martı́nez-Moya, Manuel; Alonso, Carolina; Martín, Beatriz Barrantes; Rodríguez‐Alonso, Beatriz; García-Peiró, Agustín; Gosálvez, Jaime

doi:10.1016/s1698-031x(12)70039-0

Cited by 4 publications

(7 citation statements)

References 36 publications

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“…Another group of patients that could have increased oxidative stress are patients with leukocytospermia (Saleh et al ., ), but it has been described that they do not show an increase in the nuclear degraded subpopulation (Rodriguez et al ., ; Gosalvez et al ., ). This fact could be explained in the sense that they might not have testicular affectation, as with varicocele, and because of that, they would present good sperm nuclear DNA compaction.…”

Section: Discussionmentioning

confidence: 99%

“…In a recent paper, a relation has been established between varicocele affectation and the degradation index using the SCD test. Degradation index values above 0.3 have been proposed as a marker for varicocele affectation (Rodriguez et al ., ; Gosalvez et al ., ). Degradation index data showed lower values than 0.3 in untreated samples and in hydrogen peroxide treatments with and without DTT.…”

Section: Discussionmentioning

confidence: 99%

“…Another parameter calculated has been the degradation index, which is defined as %nuclear degraded spermatozoa/%SDF (Rodriguez et al ., ; Gosalvez et al ., ).…”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, although this sperm subpopulation is present in all semen samples in a low percentage, it also shows a significant increase in varicocele patients and rearranged genome carriers (Enciso et al ., ; Garcia‐Peiró et al ., ). In this sense, it has recently been described that a degradation index (%nuclear degraded spermatozoa/%SDF) by the SCD test higher than 0.3 is associated with a varicocele affectation (Rodriguez et al ., ; Gosalvez et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…(), using controlled DTT incubations to induce nuclear sperm decompaction, showed that the HDS subpopulation is associated with an immature sperm chromatin status. Recently, some works have determined the presence of the degraded subpopulation in different infertile patients using the SCD test (Enciso et al ., ; Garcia‐Peiró et al ., ; Rodriguez et al ., ; Gosalvez et al ., ). Nevertheless, little is known about their aetiology in semen samples.…”

Section: Introductionmentioning

confidence: 99%

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Nuclear degraded sperm subpopulation is affected by poor chromatin compaction and nuclease activity

et al. 2014

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There is an interest in the nuclear degraded sperm subpopulation because, although it is present in a low percentage in all semen samples, patient groups such as varicocele and rearranged genome carriers show high levels of these degraded spermatozoa. This study is designed with two objectives in mind: first, incubations of H2 O2 and nuclease on DTT-treated and untreated samples to show the aetiology of this subpopulation and second, assessment of the correlation between the protamine ratio and nuclear degraded spermatozoa. A very high increase in the nuclear degraded subpopulation has been found with nuclease incubation, and it is even higher when it has been merged with nuclear decompaction using DTT. Alternatively, incubation with H2 O2 with and without DTT did not show such a significant increase in nuclear degraded spermatozoa. The protamine ratio correlated with this subpopulation, showing, in patients, that poor nuclear compaction would turn the sperm susceptible to degradation. Then, the assessment of nuclear degraded spermatozoa might not be only a measure of DNA degradation but also an indicator of chromatin compaction in the spermatozoa. Different patient groups would fit this model for sperm nuclear degradation, such as varicocele patients, who show a high percentage of immature spermatozoa and nuclear degraded spermatozoa, and reorganised genome carriers, where reorganisation might also cause poor chromatin compaction on the sperm nucleus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Another parameter calculated has been the degradation index, which is defined as %nuclear degraded spermatozoa/%SDF (Rodriguez et al ., ; Gosalvez et al ., ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nuclear degraded sperm subpopulation is affected by poor chromatin compaction and nuclease activity

et al. 2014

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¿A qué nivel se produce la fragmentación del ADN en pacientes infértiles con varicocele?

Campos-Hernández

Castro

López

et al. 2012

Revista Internacional de Andrología

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Oral antioxidant treatment partly improves integrity of human sperm DNA in infertile grade I varicocele patients

et al. 2015

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Infertile males with varicocele have the highest percentage of sperm cells with damaged DNA, compared to other infertile groups. Antioxidant treatment is known to enhance the integrity of sperm DNA; however, there are no data on the effects in varicocele patients. We thus investigated the potential benefits of antioxidant treatment specifically in grade I varicocele males. Twenty infertile patients with grade I varicocele were given multivitamins (1500 mg L-Carnitine, 60 mg vitamin C, 20 mg coenzyme Q10, 10 mg vitamin E, 200 μg vitamin B9, 1 μg vitamin B12, 10 mg zinc, 50 μg selenium) daily for three months. Semen parameters including total sperm count, concentration, progressive motility, vitality, and morphology were determined before and after treatment. In addition, sperm DNA fragmentation and the amount of highly degraded sperm cells were analyzed by Sperm Chromatin Dispersion. After treatment, patients showed an average relative reduction of 22.1% in sperm DNA fragmentation (p = 0.02) and had 31.3% fewer highly degraded sperm cells (p = 0.07). Total numbers of sperm cells were increased (p = 0.04), but other semen parameters were unaffected. These data suggest that sperm DNA integrity in grade I varicocele patients may be improved by oral antioxidant treatment.

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Varicocele, leucocitospermia y su impacto en la fragmentación del ADN espermático

Cited by 4 publications

References 36 publications

Nuclear degraded sperm subpopulation is affected by poor chromatin compaction and nuclease activity

Nuclear degraded sperm subpopulation is affected by poor chromatin compaction and nuclease activity

¿A qué nivel se produce la fragmentación del ADN en pacientes infértiles con varicocele?

Oral antioxidant treatment partly improves integrity of human sperm DNA in infertile grade I varicocele patients

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