Varicella-zoster virus vaccine DNA differs from the parental virus DNA

Ecker, Joseph R.; Hyman, Richard W.

doi:10.1128/jvi.40.1.314-318.1981

Cited by 19 publications

(4 citation statements)

References 24 publications

(7 reference statements)

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“…Additional passages in or in guinea pig embryo cells (20 passages) produced only minor variations. Similarly, Ecker and Hyman [1981] noted only minor differences in the restriction patterns of the vaccine DNA compared to a parent strain. The cleavage patterns from the two Ellen strains are also very similar despite a difference of 30 in vitro passages (Fig.…”

Section: Discussionmentioning

confidence: 95%

Restriction endonuclease analysis of varicella‐zoster vaccine virus and wild‐type dnas

Martin¹,

Dohner²,

Wellinghoff³

et al. 1982

Journal of Medical Virology

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The DNA from several clinical isolates of varicella-zoster virus (VZV) were compared with the DNA from the vaccine strain VZV using three restriction endonucleases: BamHI, BgII, and HpaI. When electrophoresed through an agarose gel, the vaccine DNA digestion pattern was significantly different from the digestion patterns of the wild-type DNAs. Variations in the digestion pattern of the separate clinical isolates were also observed.

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Section: Discussionmentioning

confidence: 95%

Restriction endonuclease analysis of varicella‐zoster vaccine virus and wild‐type dnas

Martin¹,

Dohner²,

Wellinghoff³

et al. 1982

Journal of Medical Virology

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“…Electrophoresis was carried out at 3.1 V/cm for 16 to 24 h. After staining with ethidium bromide (2 p.g/ml) for 1 h at room temperature, gels were photographed under UV illumination on Polaroid type 667 film. Gels to be used for determining the molecular weights and molarities of GPCMV DNA fragments contained DNA radiolabeled in vivo and were enhanced by fluorography with 1.0 M sodium salicylate (Mallinkrodt) as described previously (8,14), dried on filter paper at 37°C, and exposed on Kodak XAR-film at -76°C. Fluorographs were scanned at 550 nm with a Beckman DU-8 spectrophotometer, and the areas under the peaks were quantitated.…”

Section: Methodsmentioning

confidence: 99%

Characterization of guinea pig cytomegalovirus DNA

Isom¹,

Gao²,

Wigdahl³

1984

J Virol

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The genome of guinea pig cytomegalovirus (GPCMV) was analyzed and compared with that of human cytomegalovirus (HCMV). GPCMV and HCMV DNAs were isolated from virions and further purified by CsCl centrifugation. Purified GPCMV DNA sedimented as a single peak in a neutral sucrose gradient and was infectious when transfected into guinea pig embryo fibroblast cells. The cytopathology was characteristic of that seen after infection with GPCMV. Virus DNA purified from virions isolated from infected GPEF or 104C1 cells had a CsCl buoyant density of 1.713 g/cm3, which corresponds to a guanine plus cytosine content of 54.1%. The CsCl buoyant density of GPCMV DNA was slightly less than that of HCMV DNA (1.716 g/cm3), but sufficiently different so that the two virus DNA peaks did not coincide. GPCMV DNA cosedimented with T4 DNA in a neutral sucrose gradient. Restriction endonuclease cleavage of GPCMV or HCMV DNAs with HindIll, XbaI, or EcoRI yielded fragments easily separable by agarose gel electrophoresis and ranging from 1.0 x 106 to 25.8 x 106 daltons. The number, size, and molarity of GPCMV DNA fragments generated by restriction enzymes were determined. Hybridization of restriction endonucleasecleaved GPCMV DNA with radioactively labeled HCMV DNA and, conversely, hybridization of restriction endonuclease-cleaved HCMV DNA with radioactively labeled GPCMV DNA indicated sequence homology between the two virus DNAs.

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“…Restriction endonuclease fingerprinting (REFP) of herpesvirus DNA has been used to differentiate virus isolates and has been applied to varicella-zoster virus (VZV) strains [Ecker and Hyman, 1981;Gershon et al, 1984;Hayakawa et al, 1984Hayakawa et al, ,1986Pichini et al, 1983;Straus et al, 1983Straus et al, , 1984Williams et al, 1985;Zweerink et al, 19811. VZV infection manifests clinically either as chickenpox or zoster.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Oka varicella vaccine strain from wild varicella‐zoster virus strains isolated from vaccinees and household contact

Shiraki

Horiuchi

Asano

et al. 1991

Journal of Medical Virology

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The Oka varicella vaccine strain can be differentiated from wild-type strains by its unique restriction endonuclease fingerprinting (REFP: HpaI-K and EcoRI-P) pattern of the gpV-coding region of the varicella-zoster virus (VZV) genome. VZV-DNAs from patients with complicated clinical courses related to vaccination were examined to determine whether they were vaccine-derived or wild-type. A virus was isolated from a one year-old boy with acute lymphocytic leukemia (ALL) who developed typical varicella 28 days after vaccination (case A). Another virus was isolated from a four-year-old boy without clinical symptoms following household contact with varicella patients at the age of two months, and he developed zoster 14 months after vaccination (case B). Also, two strains (OK1 and OK2) were isolated from household contacts (mother and sister) with a vaccine with ALL in Oklahoma who developed varicella 18 days after vaccination (case C). In case C, BgII-REFP did not determine conclusively whether the two strains (OK1 and OK2) were vaccine-derived or wild-type because the patterns obtained were different from both the Oka varicella vaccine strain and American wild-type strains [Gelb et al., Journal of Infectious Diseases, 155:633-640, 1987]. All VZV strains examined in the present study were identified as wild-type by our method using HpaI-K and EcoRI-P fragments as marker fragments. Thus it is becoming evident that REFP using HpaI and EcoRI endonucleases is a convenient and reliable means of distinguishing between the Oka vaccine virus strain and wild-type viruses isolated from individuals developing vesicular rashes shortly and long after varicella vaccination.

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Varicella-zoster virus vaccine DNA differs from the parental virus DNA

Cited by 19 publications

References 24 publications

Restriction endonuclease analysis of varicella‐zoster vaccine virus and wild‐type dnas

Restriction endonuclease analysis of varicella‐zoster vaccine virus and wild‐type dnas

Characterization of guinea pig cytomegalovirus DNA

Differentiation of Oka varicella vaccine strain from wild varicella‐zoster virus strains isolated from vaccinees and household contact

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