Variations of plasma protein electrophoresis in healthy captive Green Iguanas (<i>Iguana iguana</i>)

Musilová, Anna; Knotková, Z.; Pinterova, K; Knotek, Z.

doi:10.1111/vcp.12238

Cited by 11 publications

(15 citation statements)

References 16 publications

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“…Little is known about this condition in veterinary medicine with the description of bisalbuminemia in different types of animals from amphibians ( Taricha granulosa ) to reptiles ( Iguana iguana ) and mammals 18 19. In the case of mammals, detection of bisalbuminemia has been described in clinically healthy cats and bottlenose dolphins ( Tursiops truncatus ) with hereditary bisalbuminemia 20 21…”

Section: Discussionmentioning

confidence: 99%

Bisalbuminemia in a dog with leishmaniosis after anti‐Leishmania therapeutic protocol administration: a rare condition detected in the electrophoretogram

Villanueva‐Saz

Ballesté²,

Casasnovas

et al. 2020

Vet Record Case Reports

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A 5-year-old spayed, crossbred female dog was referred for a unilateral nosebleed of 4 months’ duration. Rhinoscopy revealed significant inflammation of the nasal mucosa compatible with severe rhinitis. On the right nostril, the presence of a white patch on the mucosa was observed and a biopsy was taken. Histopathology revealed a granulomatous inflammation with the presence of neutrophils and lymphocytes. Some macrophages contained small oval microorganisms compatible with Leishmania species. The presence of Leishmania infantum was confirmed by specific immunohistochemistry, showing stained intracellular microorganisms. Abnormal clinicopathological findings included mild anaemia and hyperglobulinemia with polyclonal gammopathy. Moderate amount of antibodies to L. infantum were detected by ELISA in serum. An anti-Leishmania therapeutic protocol was established with allopurinol and meglumine antimoniate, and 4 months later, a follow-up examination was performed showing improvements in laboratory parameters with the exception of the presence of bisalbuminemia.

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Section: Discussionmentioning

confidence: 99%

Bisalbuminemia in a dog with leishmaniosis after anti‐Leishmania therapeutic protocol administration: a rare condition detected in the electrophoretogram

Villanueva‐Saz

Ballesté²,

Casasnovas

et al. 2020

Vet Record Case Reports

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“…Gels were stained with Amido Black (Sebia, Evry, France). The Preference densitometer (Sebia, Evry, France) was used to scan the electrophoretograms (Musilova et al 2015).…”

Section: Methodsmentioning

confidence: 99%

“…Results of the determination of total protein and protein fractions are shown in Table 2. The measured values were compared with reference values for healthy captive green iguanas measured in the same laboratory (Musilova et al 2015).…”

Section: Plasma Protein Electrophoresis In Green Iguana Patientsmentioning

confidence: 99%

“…albumin or prealbumin, alpha, beta and gamma globulins) have been observed in reptiles (Girling 2010). Reference values have been published for a limited number of reptile species (Deem et al 2006;Deem et al 2009;Gimenez et al 2010;Cooper-Bailey et al 2011;Proverbio et al 2012;Fiorucci et al 2013;Andreani et al 2014;Flower et al 2014, Musilova et al 2015 changes in the proportions of plasma protein fractions in reptile patients were described only in some studies (Gimenez et al 2010;Moore et al 2014). The aim Supported by the Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno (Institucionální výzkum FVL VFU Brno, VFU FVL/1650/29).…”

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confidence: 99%

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Plasma protein electrophoresis in green iguanas (Iguana iguana) suffering from hepatic, renal and inflammatory skin diseases

Knotková¹,

Musilová²,

Pinterova³

et al. 2019

Vet. Med. - Czech

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The goals of this study were to evaluate electrophoretograms in green iguanas suffering from hepatic, renal and inflammatory skin diseases. Plasma samples were obtained from 14 green iguana patients. The diagnosis was based on clinical examination, evaluation of biochemical and haematological profiles or histopathological examination. Agarose gel electrophoresis was performed using an automated HYDRASYS system. A decrease in the albumin/globulin ratio was observed in 11 out of 14 patients (three out of four patients with liver failure, one out of one patient with chronic renal insufficiency and suspected hepatopathy, two out of two patients with chronic interstitial nephritis, four out of five patients with chronic renal insufficiency and one out of two patients with dermatitis). The alpha globulin fraction was elevated in 1 out of 14 patients (patient with dermatitis). The fraction of beta globulins was increased in 8 out of 14 patients (two out of four patients with liver diseases, four out of eight patients with various forms of renal failure and two out of two patients with inflammatory skin diseases). Increased gamma globulin levels were observed in 4 out of 14 patients (one out of four patients with impaired function of the liver, two out of eight patients with renal diseases and one out of two patients with dermatitis). Changes in the electrophoretograms of green iguanas are not disease-specific, but in conjunction with other biochemical blood tests the electrophoresis of plasma proteins may be a useful indicator in the diagnosis of metabolic and infectious diseases and in the monitoring of disease progress.

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“…This type of alteration is well recognizable by capillary zone electrophoresis (CZE), whilst agarose gel electrophoresis (AGE) not always produces a clear separation of albumin fractions [ 7 , 8 ] and it sometimes needs an increased migration time [ 6 ] and buffer pH [ 9 ]. In veterinary medicine, few reports describe the presence of bisalbuminemia in domestic or wild animals: this alteration is described in amphibians [ 10 ] and, recently, the presence of bisalbuminemia was reported in 4 healthy green iguanas [ 11 ]. As regards marine mammals, only two cases of bisalbuminemia were reported in apparently healthy bottlenose dolphins ( Tursiops truncatus ): the first case was a female, approximately of 10–12 years old, captured in 1977 in the Gulf of Mexico [ 12 ] and the second was a 10 years old male [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis

et al. 2016

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BackgroundHereditary bisalbuminemia is a relatively rare anomaly characterized by the occurrence of two albumin fractions on serum protein separation by electrophoresis. In human medicine, it is usually revealed by chance, is not been clearly associated with a specific disease and the causative genetic alteration is a point mutation of human serum albumin gene inherited in an autosomal codominant pattern. This type of alteration is well recognizable by capillary zone electrophoresis (CZE), whilst agarose gel electrophoresis (AGE) not always produces a clear separation of albumin fractions. The aims of this study is to report the presence of this abnormality in two separate groups of related bottlenose dolphins and to compare the results obtained with capillary zone and agarose gel electrophoresis.ResultsSerum samples from 40 bottlenose dolphins kept under human care were analyzed. In 9 samples a double albumin peak was evident in CZE electrophoresis while no double peak was noted in AGE profile. Since only an apparently wider albumin peaks were noted in some AGE electrophoretic profiles, the ratio between base and height (b/h) of the albumin peak was calculated and each point-value recorded in the whole set of data was used to calculate a receiver operating characteristic curve: when the b/h ratio of albumin peak was equal or higher than 0.25, the sensitivity and specificity of AGE to detect bisalbuminemic samples were 87 and 63 %, respectively. The bisalbuminemic dolphins belong to two distinct families: in the first family, all the siblings derived from the same normal sire were bisalbuminemic, whereas in the second family bisalbuminemia was present in a sire and in two out of three siblings.ConclusionsWe report for the first time the presence of hereditary bisalbuminemia in two groups of related bottlenose dolphins identified by means of CZE and we confirm that AGE could fail in the identification of this alteration.

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Variations of plasma protein electrophoresis in healthy captive Green Iguanas (Iguana iguana)

Abstract: Reference intervals of the plasma protein fractions according to electrophoresis in the Green Iguana can be affected by seasonal changes and sex of animals. It should be taken into account when clinical evaluation is performed.

Cited by 11 publications

References 16 publications

Bisalbuminemia in a dog with leishmaniosis after anti‐Leishmania therapeutic protocol administration: a rare condition detected in the electrophoretogram

Bisalbuminemia in a dog with leishmaniosis after anti‐Leishmania therapeutic protocol administration: a rare condition detected in the electrophoretogram

Plasma protein electrophoresis in green iguanas (Iguana iguana) suffering from hepatic, renal and inflammatory skin diseases

Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis

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