Variations of glucosinolates in kale leaves (Brassica oleracea var. acephala) treated with drought-stress in autumn and spring seasons

Abstract

: The present study aimed to investigate the effects of drought stress on the accumulation of glucosinolates (GSLs) in the leaves of Kale cultivated in autumn and spring. HPLC analysis guided to identify seven GSLs including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrasscin. Quantification of GSLs revealed that the contents of sigirin was the highest (45%) followed by the level of progoitrin (24%) in terms of total GSLs. The ranges of total GSL contents was 1.16 (84)-15.88 (89 DAS, µmol/g dry wt. (DW)) in treatment plot and 1.23 (84)-7.05 (74 DAS, µmol/g dry wt.) in control plot showed the enhancement in the contents of GSLs in treatment than in the control plot. The present results evidenced that the variation of total GSL contents were depending on the harvest period. In 105 DAS, comparatively no differences in the GSL contents on each sample in autumn season, whereas in spring season, although there was decrease in the GSLs tendency from 74 DAS to 84 DAS in both control and treatment plot, the GSL contents of treatment plot was dramatically increased in 89 DAS. In treatment plot, the GSL contents on 89 DAS (1.16) was 15 fold higher to 84 DAS (15.88 µmol/g DW). The variation in the contents of GSL in spring and autumn did not documented significant differences because of their differences in the growth time and cultivation conditions. In conclusion, the GSL contents in kale was likely to be affected by drought stress treatment. Scrutiny and further research for exact relation between drought stress and GSL contents in kale should be needed.

Effects of Electro-conductivity on Growth of Beet and Turnip in the Reclaimed Land Soil

“…GSLs류 물질들은 그 기본구조에 β-D-glucopyranose를 갖 고 있으며, 7종류 아미노산(alanine, methione, waline, leucine, isoleucine, phenylalanine, tryptophan)으로부터 생합성된다 (Fahey et al, 2001;Halkier and Gershenzon, 2006;Jeong et al, 2015). 식물의 조직이 벌레나 바람 등에 의하여 물리적으로 손상될 때, GSLs는 식물체 내에 있는 myrosinase의 가수분해에 의하여 isothiocyanate, thiocyanate 및 nitrile 등을 생성한다.…”

“…GSL 추출 및 HPLC 분석은 여러 논문을 참고하여 시행 하였다 (Jeong et al, 2015;Kim et al, 2015;Park et al, 2017). GSL 분석은 Inertsil ODS-3 column(150×3.0 mm I.D., particle size 3 μm), 가드 컬럼은 Inertsil ODS-2 Cartridge Guard column E(10×2.0 mm I.D., particle size 5 μm) (GL Science, Tokyo, Japan)을 사용하였고 HPLC ( (Sobhanian et al, 2011;Hong et al, 2015).…”

“…GSL 분석은 Inertsil ODS-3 column(150×3.0 mm I.D., particle size 3 μm), 가드 컬럼은 Inertsil ODS-2 Cartridge Guard column E(10×2.0 mm I.D., particle size 5 μm) (GL Science, Tokyo, Japan)을 사용하였고 HPLC ( (Sobhanian et al, 2011;Hong et al, 2015). 케일은 수 분 스트레스 기간이 증가할수록 수분 함량이 감소하였고 (Jeong et al, 2015), 케일의 생체중도 감소하는 경향을 보였 다 (Lee et al, 2017). 저온처리하여 생장한 케일은 온실에서 재배한 케일보다 생육이 더 낮은 경향을 보였다 (Hwang et al, 2017).…”

“…브로콜리 새싹채소에서 NaCl 농도에 따라 GSL 함량 은 무처리구(NaCl 0 mmol/L)부터 60 mmol/L 까지는 감 소하다가 80, 100 mmol/L에서는 높게 나타났고, 3일차에서 5, 7일차로 갈 수로 그 함량은 낮아졌는데, 이는 NaCl이 식 물이 생장함에 따라 염에 내성이 생기기 때문이라고 고찰하 였다 (Guo et al, 2013). 케일이 수분 스트레스를 더 심하게 받을수록 급격히 많은 양의 GSLs를 축적하였다 (Brown et al, 2002;Jeong et al, 2015).…”