Variations of bacterial-specific activity with cell size and nucleic acid content assessed by flow cytometry

Lebaron, Philippe; Servais, Pierre; Baudoux, Anne‐Claire; Bourrain, Muriel; Courties, Claude; Parthuisot, Nathalie

doi:10.3354/ame028131

Cited by 152 publications

(159 citation statements)

References 23 publications

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“…It has previously been demonstrated that HDNA cells represent the active component of bacterioplankton communities, while LDNA cells are likely to consist of dormant or dead bacteria (Gasol et al, 1999;Lebaron et al, 2001Lebaron et al, , 2002.…”

Section: Resultsmentioning

confidence: 99%

“…Based on di¡erences in SYBR Green £uorescence (DNA content) and side scatter (an indicator of cell size), heterotrophic bacteria were divided into two distinct populations, high DNA (HDNA) and low DNA (LDNA) (Gasol et al, 1999;Lebaron et al, 2001Lebaron et al, , 2002 (Figure 1). It has previously been demonstrated that HDNA cells represent the active component of bacterioplankton communities, while LDNA cells are likely to consist of dormant or dead bacteria (Gasol et al, 1999;Lebaron et al, 2001Lebaron et al, , 2002.…”

Section: Resultsmentioning

confidence: 99%

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Flow cytometric analysis of virus-like particles and heterotrophic bacteria within coral-associated reef water

2006

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Using £ow cytometry, two distinct populations of virus-like particles (VLP) and heterotrophic bacteria were de¢ned within the 12 cm water layer immediately overlying healthy, diseased and dead acroporid corals. Bacterial abundances were similar in overlying water for all coral types, however, VLP were 30% higher above diseased corals than healthy or dead corals. Mean virus to bacteria ratios (VBR) were up to 30% higher above diseased corals than above healthy or dead coral or in distant water. Concomitant with increasing VLP concentrations within 5 cm of coral surfaces, VBR distributions were generally highest above healthy and diseased coral and depressed above dead coral. These results suggest fundamental shifts in the VLP and bacterial community in water associated with diseased corals.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Flow cytometric analysis of virus-like particles and heterotrophic bacteria within coral-associated reef water

2006

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“…Microscopic analyses and flow cytometry, combined with specific coloration techniques, can be used to characterise the morphological diversity of microbial communities, e.g. the size-distribution of microbial cells within a groundwater sample (Lebaron et al 2002).…”

Section: Methodsmentioning

confidence: 99%

Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

2006

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The current knowledge of microbial biocenoses (communities) in pristine aquifers is presented in a review, which also discusses their relevance for questions of groundwater protection. Aquifers are heterogeneous on all scales and structured in a variety of habitats. The void spaces in many aquifers are small. The biocenoses are thus predominantly composed of microorganisms and, often, microinvertebrates. Larger voids and macroorganisms occur in karst cavities. Due to the absence of light, the biocenoses depend on chemical energy resources, which are, however, scarce in non-contaminated groundwater. The microorganisms thus show small cell sizes, low population densities and reduced activity; they developed specific strategies to survive oligotrophic conditions. The review also discusses the impact of contamination on the biocenoses, and the potential use of the biocenoses or specific organisms as indicators for groundwater quality, and the limits of this approach. Bacteria are either planktonic or attached to aquifer material, which requires both fluid and solid phase sampling. Most groundwater bacteria are viable but non-culturable. Consequently, cultivation techniques give an incomplete picture of the biocenoses, while methods from molecular microbiology provide genetic fingerprints of the entire community. Different analytical methods are available to count microorganisms, identify species, characterise microbial diversity, and measure activity.Résumé Cette revue expose l'état actuel des connaissances concernant les biocénoses microbiennes présentes dans les aquifères oligotrophes. L'impact d'une contamination sur les biocénoses est discuté, ainsi que le potentiel que Les bactéries sont planctoniques ou attachées aux matériaux de l'aquifère, ce qui demande unéchantillonnagè a la fois de l'eau et du substrat. De nombreuses méthodes sont aujourd'hui disponibles pour le comptage, l'identification et la caractérisation de la diversité, ainsi que la mesure des activités des organismes des aquifères. Comme la grande majorité des bactéries est viable mais non cultivable, les techniques de cultures actuelles ne donnent qu'une image incomplète des communautés, alors que les méthodes moléculaires développées récemment offrent la possibilité d'obtenir un profil de la communauté plus complet.Resumen Se presentauna reseña crítica del conocimiento actual de biocenosis microbiana (comunidades) en acuíferos prístinos la cual también discute su relevancia en términos de protección de aguas subterráneas. Los acuíferos son heterogéneos en todas las escalas y estructurados en una variedad de habitats. Los espacios vacíos en muchos acuíferos son pequeños. La biocenosis está por lo tanto compuesta predominantemente por microorganismos y, frecuentemente, microinvertebrados. Espacios más grandes y macroorganismos ocurren en cavidades kársticas. Debido a la ausencia de luz la biocenosis depende de recursos energéticos químicos los cuales, sin embargo, son escasos en agua subterránea no contaminada. Los microorganismos muestran...

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“…Also cytometric counts revealed the heterogeneity and differences in bacterioplankton populations allowing the distinction of two general groups: bacteria with high apparent nucleic acid content (HNA) and bacteria with low apparent nucleic acid content (LNA) (1,14,22,26,30,45). The HNA bacteria were considered the most active members of a given community while LNA were regarded as inactive, dead or dormant cells (17,26,27). HNA and LNA were also considered to be members of the same phylotypes (11,38).…”

Section: Introductionmentioning

confidence: 99%

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean

Andrade¹,

Gonzalez²,

Rezende³

et al. 2007

Braz. J. Microbiol.

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Bacterioplankton was studied in a large area of Southwest Atlantic Ocean between 13 and 25ºS and 28 and 42ºW. Samples were collected in 108 stations at 20 m depth. Bacteria were enumerated by flow cytometry after nucleic acid staining with syto13 and two subgroups were differentiated: low nucleic acid content (LNA) and high nucleic acid content (HNA) bacteria. Total bacterial numbers varied from 0.37 to 5.53 10 5 cells mL -1. HNA cells represented 15 to 70% of the total number while LNA cells represented 30 to 85%. Heterotrophic bacterial production was determined by incorporation of tritiated leucine and ranged from 2.7 to 171.07 ng C L -1 h -1. No significant correlation was found between abundance and production. Nevertheless with support of multivariate analysis between bacterial abundance, bacterial production, chlorophyll a and other oceanographic data the distribution of the groups in two different oceanic provinces could be explained by nutrient availability. HNA bacteria accounted for the high percentage of cells found in the area north of 19ºS, linked to higher temperature waters and riverine nutrients inputs. LNA bacteria were the dominant cells south of this latitude and were correlated to the higher values of nitrate found for the same area.

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Variations of bacterial-specific activity with cell size and nucleic acid content assessed by flow cytometry

Cited by 152 publications

References 23 publications

Flow cytometric analysis of virus-like particles and heterotrophic bacteria within coral-associated reef water

Flow cytometric analysis of virus-like particles and heterotrophic bacteria within coral-associated reef water

Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean

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