Variations Induced in Electrophoretic Pattern of Haemolymph Proteins of Flesh Fly, Sarcophaga Argyrostoma (Diptera: Sarcophagidae) Larvae Challenged With Hydrogen Peroxide.

Mahmoud, S. A.; Moselhy, Walaa A.; El-Khashab, L.A.A.; Abdelbaset, B.Z.; Seufi, AlaaEddeen M.

doi:10.21474/ijar01/1759

Cited by 3 publications

(1 citation statement)

References 7 publications

(7 reference statements)

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“…Similarly, the present study revealed a significant antioxidant capacity and DPPH radical scavenging of the injected M. domestica larval hemolymph. These may be associated with the increased antioxidant capacity of the hemolymph over time similarly to previous reported increase or induction of new proteins to overcome the stress of hydrogen peroxide in Sarcophaga argyrostoma larvae [103], and antibacterial proteins in larval hemolymph of Spodoptera eridania after LPS injection [104]. Collectively, based on current results, the further purification of hemolymph fractions and the separation of certain compounds and proteins should be performed to investigate the mechanisms of their antioxidant and cytotoxic capacities in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 74%

Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

et al. 2020

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The usage of insects as a sustainable and functional natural products resource is a new promise in complementary and alternative medicine. The present study aimed to investigate the ability of Musca domestica (housefly) larval hemolymph (insect blood) to display the enhanced in vitro antioxidant and cytotoxic effects. The oxidative stress (OS) was elicited by inducing lipopolysaccharides (LPS) treatment as an exogenous stressor. Determination of superoxide dismutase 1 (SOD1), glutathione (GSH), malondialdehyde (MDA) and total antioxidant capacity (TAC), and mRNA and protein expressions of SOD1, was investigated as confirmatory markers of oxidative stress induction. Cytotoxicity on cancerous MCF-7 and normal Vero cells were also evaluated using an MTT assay at 24 h post-injection. The injection of LPS induced a significant (p < 0.05) increase in SOD, GSH and TAC, whereas, the MDA was diminished. Hemolymph was collected from normal and treated larvae after 6, 12 and 24 h. The M. domestica superoxide dismutase (MdSOD1) transcripts were significantly (p < 0.05) upregulated 6 and 12 h post-treatment, while a significant downregulation was observed after 24 h. Western blot analysis showed that MdSOD1 was expressed in the hemolymph of the treated larvae with an increase of 1.2 folds at 6 and 12 h and 1.6 folds at 24 h relative to the control group. LPS-treated larval hemolymphs exhibited significant cytotoxicity with respect to the untreated ones against MCF-7 while Vero cells showed no cytotoxicity for both hemolymphs. The DPPH free radical scavenging activity was examined and a significant antioxidant potential potency was observed at 6 h (50% maximal inhibitory concentration (IC50): 63.3 ± 3.51 µg/mL) when compared to the control M. domestica larval hemolymph (IC50: 611.7 ± 10.41 µg/mL). Taken together, M. domestica larval hemolymph exhibited enhanced antioxidant and consequently increased cytotoxic capacities under stressed conditions.

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Section: Discussionsupporting

confidence: 74%

Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

et al. 2020

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Larvicidal and biochechemical effects of methanol leaf extract and biosynthesized silver nanoparticles using Nerium oleander L. (Apocynaceae) against Lucilia sericata M. (Diptera: Calliphoridae)

Abdel-Meguid

2022

Int J Trop Insect Sci

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Insects as a Prospective Source of Biologically Active Molecules and Pharmaceuticals—Biochemical Properties and Cell Toxicity of Tenebrio molitor and Zophobas morio Cell-Free Larval Hemolymph

Knežić,

Avramov,

Tatić

et al. 2024

IJMS

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Insects are of great interest as novel sources of alternative proteins and biologically active compounds, primarily anticancer agents. Protein-rich insect larval hemolymph is a prospective candidate for pharmaceutical and food industry-related research. In this study, selected biochemical properties and cell toxicity of larval hemolymph from two mealworm species, Tenebrio molitor and Zophobas morio, were analyzed. Total proteins and carbohydrates, antioxidant capacity, and the level of lipid peroxidation were determined. Human cancer (U-87) and normometabolic (MRC-5) cells were treated with different concentrations of larval hemolymph proteins, and the effects on cell viability were assayed 24, 48, and 72 h after treatments. Z. morio hemolymph was shown to be richer in total proteins, showing a higher antioxidant capacity and lipid peroxidation level than T. molitor hemolymph, which was richer in total carbohydrates. Cytotoxicity assays showed that T. molitor and Z. morio hemolymphs differently affect the viability of U-87 and MRC-5 cells in cell type-, dose-, and time-dependent manners. Hemolymph from both species was more cytotoxic to U-87 cells than to MRC-5 cells, which was particularly prominent after 48 h. Additionally, a more potent cytotoxic effect of Z. morio hemolymph was observed on both cell lines, likely due to its higher antioxidant capacity, compared to T. molitor hemolymph.

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Variations Induced in Electrophoretic Pattern of Haemolymph Proteins of Flesh Fly, Sarcophaga Argyrostoma (Diptera: Sarcophagidae) Larvae Challenged With Hydrogen Peroxide.

Cited by 3 publications

References 7 publications

Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

Larvicidal and biochechemical effects of methanol leaf extract and biosynthesized silver nanoparticles using Nerium oleander L. (Apocynaceae) against Lucilia sericata M. (Diptera: Calliphoridae)

Insects as a Prospective Source of Biologically Active Molecules and Pharmaceuticals—Biochemical Properties and Cell Toxicity of Tenebrio molitor and Zophobas morio Cell-Free Larval Hemolymph

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