Variations in the leukocyte and cytokine profiles between placental and maternal circulation in pregnancy-associated malaria

Okamgba, Okezie C; Ifeanyichukwu, Martin; Ilesanmi, Ayodele O; Chigbu, Lawrence N

doi:10.2147/rrtm.s137829

Cited by 20 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the maternal peripheral level of IL-17A and the placental and neonate IFN-γ levels were not signi cantly different due to the infection. Several previous studies have shown elevated levels of type 2 anti-in ammatory cytokines IL-10 and IL-4 in the malariainfected group [13,15,23,25,30,31]. Our ndings were different from those reported by Bayoumi et al [11] and Chêne and et al [12] who found higher levels of IL-4 and IL-10 in the non-infected group.…”

Section: Discussioncontrasting

confidence: 99%

Impact of placental malaria on maternal, placental and foetal cord responses and its role in pregnancy outcomes in women from Blue Nile State, Sudan

Omer

Franco-Jarava²,

Noureldien

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Sequestration of Plasmodium falciparum infected cells in the placenta results in placental malaria (PM). It activates a mother's immune cells and induces secretion of inflammatory cytokines, which might influence pregnancy outcomes. This study aims to investigate the inflammatory environment in maternal peripheral, placental, and umbilical cord blood in response to PM and the extent to which this may influence maternal haemoglobin levels and birth weight Methods A total of 185 consenting Sudanese women from Blue Nile state were enrolled in a cross sectional conducted between Jan 2012-Dec2005. Malaria infection in the collected samples was determined microscopically, and ELISA was used to measure the plasma levels of the antibodies, IL-4, IL-6, IL-10, IL-17A, and INF γ in the collected positive and negative malaria samples. Results Elevated levels of antibodies, IL-4 and IL-10 and reduced levels of IL-6 were detected in the malaria positive samples in comparison to the negative ones in the three types of samples investigated. Maternal antibodies, IL-4 and IL-10 were significantly higher in the samples collected from the PM infected group compared to the non-infected control (P < 0.001). While the absence of PM was significantly associated with the IL-6 and maternal IFN-γ levels, maternal IL-17A, placental and umbilical cord IFN-γ levels showed no significant difference (P = 0.214, P = 0.065, P = 0.536 respectively) due to infection. Haemoglobin level and birth weight were increased in the group with high levels of IL-6 and IL-17A but not in the group with IL-4 and IL-10 levels. Only maternal peripheral FN-γ level was significantly positively correlated with maternal hemoglobin (r = 0.171, P = 0.020), and baby birth weight (r = 0.233, P = 0.001). Conclusion These results suggest that PM cross-reacts with the mother’s immune response and impairs her cytokine profile, which might alter maternal haemoglobin levels and the baby's birth weight.

show abstract

Section: Discussioncontrasting

confidence: 99%

Impact of placental malaria on maternal, placental and foetal cord responses and its role in pregnancy outcomes in women from Blue Nile State, Sudan

Omer

Franco-Jarava²,

Noureldien

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, the maternal peripheral level of IL-17A and the placental and neonate IFN-γ levels were not signi cantly different due to the infection. Several previous studies have shown elevated levels of type 2 anti-in ammatory cytokines IL-10 and IL-4 in the malariainfected group [12,13,15,23,25,30,31]. Conversely, the present results were different from the previous ndings in an area with unusable malaria transmission in Sudan, where higher levels of IL-4 and IL-10 in the non-infected group was reported [11].…”

Section: Discussioncontrasting

confidence: 99%

Impact of placental malaria on maternal, placental and foetal cord responses and its role in pregnancy outcomes in women from Blue Nile State, Sudan

Omer

Franco-Jarava²,

Noureldien

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The sequestration of Plasmodium falciparum infected cells in the placenta results in placental malaria (PM). It activates the mother's immune cells and induces secretion of inflammatory cytokines, which might influence pregnancy outcomes. This study aims to investigate the inflammatory environment in maternal peripheral, placental, and umbilical cord blood in response to PM and the extent to which this may influence maternal haemoglobin levels and birth weight.Methods A total of 185 consenting Sudanese women from Blue Nile state were enrolled in a cross sectional conducted between Jan 2012-Dec 2005. Malaria infection in the collected samples was determined microscopically, and ELISA was used to measure the plasma levels of the antibodies, IL-4, IL-6, IL-10, IL-17A, and INF γ in the collected positive and negative malaria samples. Results Elevated levels of antibodies, IL-4 and IL-10 and reduced levels of IL-6 were detected in the malaria positive samples in comparison to the negative ones in the three types of samples investigated. Maternal antibodies, IL-4 and IL-10 were significantly higher in the samples collected from the PM infected group compared to the non-infected control (P < 0.001). While the absence of PM was significantly associated with the IL-6 and maternal IFN-γ levels, maternal IL-17A, placental and umbilical cord IFN-γ levels showed no significant difference (P=0.214, P=0.065, P=0.536, respectively) due to infection. Haemoglobin level and birth weight were increased in the group with high levels of IL-6 and IL-17A, but not in the group with IL-4 and IL-10 levels. While significantly negative correlation was found between IFN-γ levels and birth weight for all three types of samples, only maternal peripheral FN-γ level was significantly positively correlated with maternal haemoglobin (r= 0.171, P =0.020).Conclusion These results suggest that PM cross-reacts with the mother’s immune response and impairs her cytokine profile, which might alter maternal haemoglobin levels and the baby's birth weight.

show abstract

“…Findings of this study showed that IFNγ, TNFα, IL-4, IL-6 and IL-10 were elevated in infected placentas as opposed to that of uninfected placentas. This finding is in consonance with studies by (Sharma and Shukla, 2017;Okamgba et al, 2018;Lima et al, 2019) who observed that IFNγ and TNFα were increased in the parasite infected than the uninfected placenta. On the contrary, it did not agree with the finding of Bayoumi et al (2009) who observed that IFNγ, IL-4 and IL-10 were elevated in uninfected than infected placentas and Yasnot et al (2013) who observed that IL-6 and IL-10 were decreased in the infected than the uninfected placenta.…”

Section: Discussionsupporting

confidence: 92%

Association between parasite density and cytokines in malaria infected human placentas

Okamgba

2021

Ann Med Lab Sci

View full text Add to dashboard Cite

Background: Placental malaria is a major cause of infection induced adverse conditions in pregnancy and is attributed to the sequestration of malaria parasite in the intervillous space. We investigated if any relationship exists between the parasite density and cytokines in malaria parasite infected human placentas. Methods: Sixty (60) malaria parasite infected placentas from apparently healthy immediate post-partum women and 40 malaria parasite uninfected placentas which served as control were studied. Blood from the human placenta was aseptically collected and tested for HIV and malaria parasite using standard methods. Interferon-Gamma (IFNγ), Tumor Necrosis Factor alpha (TNFα), Interleukin-4 (IL-4), Interleukin-6 (IL-6) and Interleukin-10 (IL-10) were measured by Enzyme-Linked Immunosorbent Assay (ELISA) technique. Data were analysed using appropriate statistical tools. Results: The result revealed P. falciparum with a mean parasite density of 762.47±459.62 parasite/µl of blood. The mean±SD (11.71±6.55pg/ml) and 55.57±43.13 pg/ml for IFNγ and IL-10 respectively for infected placenta was statistically higher on comparison with 5.58±2.86 pg/ml and 16.60±4.88 pg/ml for IFNγ and IL10 respectively for uninfected human placenta (P<0.05). Positive correlation existed between parasite density and IL-6 (r = 0.59, p = 0.001) and between parasite density and IL-10 (r =0.41, p=0.024). Conclusion: The study showed upregulated levels of IL-6 and IL-10 which indicates disruption of normal immune balance in the parasite infected placenta and the amount of IL-6 and IL-10 secreted could reflect the level of parasitaemia and could serve for diagnostic assessment of placental malaria.

show abstract

Variations in the leukocyte and cytokine profiles between placental and maternal circulation in pregnancy-associated malaria

Cited by 20 publications

References 29 publications

Impact of placental malaria on maternal, placental and foetal cord responses and its role in pregnancy outcomes in women from Blue Nile State, Sudan

Impact of placental malaria on maternal, placental and foetal cord responses and its role in pregnancy outcomes in women from Blue Nile State, Sudan

Impact of placental malaria on maternal, placental and foetal cord responses and its role in pregnancy outcomes in women from Blue Nile State, Sudan

Association between parasite density and cytokines in malaria infected human placentas

Contact Info

Product

Resources

About