VARIATIONS IN PEROXIDASE ISOZYMES OF &lt;i&gt;ORYZA PERENNIS&lt;/i&gt; AND &lt;i&gt;O. SATIVA&lt;/i&gt;

Chu, Yaw-En

doi:10.1266/jjg.42.233

Cited by 43 publications

(17 citation statements)

References 19 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The leaf-blade isozymes of peroxidase, acid phosphatase, and esterase were analysed by starch-gel electrophoresis method (Chu, 1967;Chu and Oka, 1967). For this, homogenates were made of fresh leaves 3 to 5 days after full expansion.…”

Section: Methodsmentioning

confidence: 99%

“…Its importance in maintaining genetic variability in the populations of predominantly self-pollinated plants was demonstrated by Allard and co-workers (1962;1963). Following Anderson ( 1949), many workers have investigated natural hybrids in different plant groups, in order to analyze the structure of hybrid populations (Brehm and Ownbey, 1965;Levin, 1963;1967;Ornduff, 1964;Stutz and Thomas, 1964;etc. ) or to estimate the adaptability of hybrid derivatives to different environments (Benson et aI., 1967;Briggs, 1962).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

INTROGRESSION ACROSS ISOLATING BARRIERS IN WILD AND CULTIVATED ORYZA SPECIES

Chu¹,

Oka²

1970

Evolution

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

INTROGRESSION ACROSS ISOLATING BARRIERS IN WILD AND CULTIVATED ORYZA SPECIES

Chu¹,

Oka²

1970

Evolution

Self Cite

View full text Add to dashboard Cite

“…Yoshitake (1969) reported the isozyme variation of acid phosphatase in the silkworm in relation to its speciation, while Chu (1967) and Shahi et al (1969) compared isozymes of several enzymes among various species of the genus Oryza. Bhatia (1968) and Jaaska (1969Jaaska ( , 1970) studied esterase, acid phosphatase and alcohol dehydrogenase in some Triticum and Aegilops species.…”

mentioning

confidence: 99%

ISOZYME VARIATIONS IN AEGILOPS AND TRITICUM. I. ESTERASE ISOZYMES IN AEGILOPS STUDIED USING THE GEL ISOELECTROFOCUSING METHOD

Nakai

Tsunewaki

1971

The Japanese journal of genetics

View full text Add to dashboard Cite

The study of isozyme variations has become a useful tool for investigating phylogenetic relationships among related species. Yoshitake (1969) reported the isozyme variation of acid phosphatase in the silkworm in relation to its speciation, while Chu (1967) and Shahi et al. (1969) compared isozymes of several enzymes among various species of the genus Oryza. Bhatia (1968) and Jaaska (1969Jaaska ( , 1970) studied esterase, acid phosphatase and alcohol dehydrogenase in some Triticum and Aegilops species. The gel isoelectrof ocusing method developed by Leaback and Rutter (1968) and others, gives a sharp zymogram, because carrier ampholites added to the gel form a pH gradient after electrophoresis and each isozyme is concentrated at a specific site, whose pH value corresponds to the isoelectric point of the respective isozyme. Consequently, this method effectively separates individual isozyme fractions by their specific isoelectric points along the pH gradient of the gel. Using this method, we previously investigated esterase isozymes in wheat and four Aegilops species (Nakai et al. 1969). Results encouraged us to extend the work to all species of Aegilops, whose genomic relationships have been thoroughly investigated (Kihara 1954(Kihara , 1957. Thus, the present experiment was carried out, in which a carrier ampholite with a pH range of 5-8 (a narrower range than that used in the previous work) was used, because most esterase isozymes in this genus have isoelectric points within this pH range.

show abstract

“…Before or at the heading time, the third leaf blades from the top were sampled to make the homogenates. Peroxidase isozymes were observed by starch-gel electrophoresis method described by Endo (1966) or Chu (1967). The isozyme bands migrating towards the anode and cathode were numbered 1A, 2A, ... and 1C, 2C, ..., respectively.…”

Section: Methodsmentioning

confidence: 99%