Variations in Gut Microbiota of Siberian Flying Squirrels Correspond to Seasonal Phenological Changes in Their Hokkaido Subarctic Forest Ecosystem

Liu, Po-Yu; Cheng, An Chi; Huang, Shu‐Mei; Chang, Hao-Wei; Oshida, Tatsuo; Yu, Hon‐Tsen

doi:10.1007/s00248-018-1278-x

Cited by 19 publications

(21 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The timing and form of this seasonal microbiota restructuring was remarkably consistent across both populations and all years examined, and strongly resembled patterns detected in a two-year study of a separate UK wood mouse population (Maurice et al, 2015). Other studies in wild animals have similarly reported a dominant role of season in restructuring the gut microbiota compared to host factors (Kobayashi et al, 2006;Williams et al, 2013;Fogel, 2015;Springer et al, 2017;Amato et al, 2014a;Sun et al, 2016;Liu et al, 2019;Ren et al, 2017;Maurice et al, 2015). However, this is the first time such seasonality has been shown to have such a consistent pattern across multiple populations and years.…”

Section: Discussionsupporting

confidence: 74%

“…The copyright holder for this preprint this version posted October 16, 2021. ; https://doi.org/10.1101/2021.10.15.464528 doi: bioRxiv preprint 2014a; Sun et al, 2016;Liu et al, 2019;Ren et al, 2017;Maurice et al, 2015). However, this is the first time such seasonality has been shown to have such a consistent pattern across multiple populations and years.…”

Section: Discussionmentioning

confidence: 80%

“…A growing number of studies have revealed strong seasonal dynamics in the gut microbiota of wild animals (Kobayashi et al, 2006;Williams et al, 2013;Fogel, 2015;Springer et al, 2017;Amato et al, 2014;Sun et al, 2016;Liu et al, 2019;Ren et al, 2017;Maurice et al, 2015;Orkin et al, 2019). Pronounced seasonal microbiota dynamics have also been observed among humans leading traditional, hunter-gatherer lifestyles (Davenport et al, 2014;Smits et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Synchronous seasonality in the gut microbiota of wild wood mouse populations

Raulo

Brouard

Troitsky

et al. 2021

Preprint

View full text Add to dashboard Cite

1. The gut microbiome performs many important functions in mammalian hosts, with community composition shaping its functional role. However, what factors drive individual microbiota variation in wild animals and to what extent these are predictable or idiosyncratic across populations remains poorly understood. 2. Here, we use a multi-population dataset from a common rodent species (the wood mouse, Apodemus sylvaticus), to test whether a consistent set of 'core' gut microbes is identifiable in this species, and to what extent the predictors of microbiota variation are consistent across populations. 3. Between 2014 and 2018 we used capture-mark-recapture and 16S rRNA profiling to intensively monitor two wild UK mouse populations and their gut microbiota, as well as characterising the microbiota from a laboratory-housed colony of the same species. 4. Although broadly similar at high taxonomic levels and despite being only 50km apart, the two wild populations did not share a single bacterial amplicon sequence variant (ASV). Meanwhile, the laboratory-housed colony shared many ASVs with one of the wild populations from which it is thought to have been founded decades ago. Despite strong taxonomic divergence in the microbiota, the factors predicting compositional variation in each wild population were remarkably similar. We identified a strong and consistent pattern of seasonal microbiota restructuring that occurred at both sites, in all years, and within individual mice. While the microbiota was highly individualised, seasonal convergence in the gut microbiota among individuals occurred in late winter/early spring. 5. These findings reveal highly repeatable seasonal gut microbiota dynamics across distinct populations of this species, despite divergent taxa being involved. Providing a platform for future work to understand the drivers and functional implications of such predictable seasonal microbiome restructuring, including whether it might provide the host with adaptive seasonal phenotypic plasticity.

show abstract

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synchronous seasonality in the gut microbiota of wild wood mouse populations

Raulo

Brouard

Troitsky

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…A14-07 in 2014). These samples were sequenced in our previous study 32 ; sequence reads were retrieved from NCBI Sequence Read Archive database (accession numbers: SRX3793743-SRX3793761). 30,31 For these animals, fecal samples were collected from their cages in May, 2012 and immediately preserved in RNAlater ® (Thermo Fisher Scientific, Carlsbad, CA, USA) for subsequent bacterial genomic DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Body-size Scaling is Related to Gut Microbial Diversity, Metabolism and Dietary Niche of Arboreal Folivorous Flying Squirrels

Liu

Cheng

Huang

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

thermal homeostasis of mammals is constrained by body-size scaling. consequently, small mammals require considerable energy to maintain a high mass-specific metabolic rate (MSMR) and sustain target body temperature. in association with gut microbiota, mammalian hosts acquire absorbable molecules and fulfill their metabolic requirements. Our objective was to characterize gut microbes in wild mammals and relate those findings to host body-size scaling. Two large (Petaurista philippensis grandis and P. alborufus lena), one medium (Trogopterus xanthipes) and one small (Pteromys volans orii) species of flying squirrels (FS) were studied. Using 16S rRNA genes, 1,104 OTUs were detected from four FS, with 1.99% of OTUs shared among all FS. Although all FS gut microbiota were dominated by Firmicutes, they were constituted by different bacterial families. Moreover, Bacteroidetes accounted for up to 19% of gut microbiota in small fS, but was absent in large fS. finally, based on metagenome predictions, carbohydrate and amino acid metabolism genes were enriched in small body-size fS. in conclusion, gut microbiota compositions and predictive metabolic functions were characteristic of body-size in fS, consistent with their adaptations to folivorous dietary niches.

show abstract

“…We used the RF classification model for both datasets and followed the original paper's criteria, using the top 2. 3.2.3 | Case 2: Seasonal alterations in the flying squirrel gut microbiota are associated with forest phenology Liu et al (2019) found that the gut microbiota of wild flying squirrels in the Hokkaido forest changed seasonally with ambient temperature and forest phenology (Liu et al, 2019). A regime shift occurred in flying squirrels' gut microbiota at 20℃, at which point samples were classified into low or high temperature groups.…”

Section: Lactococcusmentioning

confidence: 99%