Variations in fine structure of renal tubular epithelium under different conditions of fixation

Maunsbach, Arvid B.; Madden, S. C.; Latta, Harrison

doi:10.1016/s0022-5320(62)80006-9

Cited by 79 publications

(16 citation statements)

References 14 publications

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“…He also noted that, in the renal medulla, the basal fi bers in the epithelial eells lining the eolleeting tubule beeome gradually less numerous as one progresses toward the tip of the papilla. Similar resulta were obtained by Djokie (1919) in the bear and by Belosaviteh (1919) (Takaki et al, 19.56;Rhodin, 1958;Miller, 196o;Latta et al, 1961;Maunsbach et al, 1962;and others). Farquhar and Palade (196:3), in their investigation of junctional complexes in a number of epithelia, found that the organization of the terminal bars varies appreciably from one segment of the nephron to another.…”

Section: On Cell Web Fibrils and Attachment Sites In General Cell Websupporting

confidence: 82%

The cell web in epithelial cells of the rat kidney

Clermont

Pereira

1966

Anat. Rec.

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The “cell web,” a supporting fibrillar component of the cytoplasm was investigated in sections of adult rat kidney stained with the tannic acid‐phosphomolybdic acid‐amido black (TPA) technique. At the apex of all tubular epithelial cells and immediately below the brush border of microvilli, the cell web formed a thin layer of tightly knit fibrils which inserted on terminal bars, the latter following a straight or sinuous course in circumscribing the cell apices. On the lateral surfaces of all tubular cells, with the exception of those lining the first segment of the proximal convoluted tubule and the whole distal convoluted tubule, some TPA stained fibrils were seen running from the terminal bars toward the base of the cells. Lastly, at the base of all tubular cells, excepting those of the macula densa and the cortical and papillary collecting ducts, some coarse fibrils, oriented circularly around the tubule, were seen close to the basement membrane. Thus, in most tubular epithelial cells, cell web fibrils were found below the apical, lateral and basal cell surfaces, making up a framework for the support of the cytoplasm. Cell Web fibrils were also observed in the parietal and visceral epithelial cells of Bowman's capsule.

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Section: On Cell Web Fibrils and Attachment Sites In General Cell Websupporting

confidence: 82%

The cell web in epithelial cells of the rat kidney

Clermont

Pereira

1966

Anat. Rec.

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“…The first point concerns the relationship between appearance of morphological ultrastructural modifications and the processing tech nique of the specimens. It is well documented in the literature [25][26][27] that only vascular perfusion of the kidney with the fixative avoids ap pearance of artifacts, particularly: (a) the apical swelling of tubular cells; (b) the collapse of tubular epithelium; (c) the shrinkage of tubular cells with widening of extracellular spaces and reduction of basal infoldings of the membranes.…”

Section: Resultsmentioning

confidence: 99%

Chronic Intoxication by Cadmium Experimentally Induced in Rabbits

Castano¹

1971

Pathobiology

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The ultrastructural alterations caused in the kidney by chronic intoxication with cadmium salts have been studied in rabbits treated for periods of from 2 weeks to 7 months. Submicroscopic observations failed to demonstrate a visible localization of cadmium in the renal parenchyma. We observed variable regressive and degenerative patterns of the tubular epithelium which were more evident after 5 and 7 months of treatment. Severe glomerular amyloidosis was seen in animals treated for 2 and 7 months, while it was minor in rabbits treated for 4 and 5 months. The possible relationship between cadmium and enzyme systems controlling protein catabolism, and that between cadmium and amyloidosis have been discussed.

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“…The kidney, in particular, has proven to be a particularly difficult organ to preserve without introducing significant artifacts (Maunsbach et al, 1962;Maunsbach, 1966a;Griffith et al, 1967;Bulger et al, 197'6). More important, it is very difficult to understand the true dynamic nature of the living glomerulus and uriniferous tubules by studying static images of preserved tissue samples.…”

Section: Discussionmentioning

confidence: 99%

Tandem Scanning Confocal Microscopy (TSCM) of normal and ischemic living kidneys

et al. 1991

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Tandem Scanning Confocal Microscopy (TSCM) allows one to section optically into and record real-time images of living organs and tissues in a noninvasive fashion. In this paper, we will present some initial TSCM observations of subcapsular nephrons in the living, intact kidneys of Munich-Wistar rats and evaluate the nephron's responses to temporary ischemia and to intravenous infusion of mannitol. The rats were anesthetized with Inactin and a laparotomy performed to expose the kidneys. Using a TSCM equipped with a 20 x water-immersion objective, we optically sectioned through the intact kidney capsule and recorded real-time images of living subcapsular glomeruli and uriniferous tubules. The proximal tubule brush border was highly reflective and allowed us to distinguish between the first and second segments of the proximal tubules as well as the distal tubules. Cellular elements of the blood could be seen passing rapidly through peritubular capillaries and individual glomerular capillary loops. With fluorescent filters in place, intravenously injected carboxyfluorescein was seen to pass through the glomerular capillary loops and then progressively through the different segments of the uriniferous tubules. Ligation of the renal artery resulted in rapid swelling of proximal tubule cells into the tubular lumens, loss of reflectiveness of the microvillous brush borders, and closure of the peritubular capillary spaces. Upon release of the ligature, the proximal tubule lumens again became patent, often opening up abruptly and in a zipper-like fashion down the length of the tubules. Increasing the glomerular filtration rate by intravenous infusion of mannitol resulted in increases in tubular luminal and perimeter dimensions. Mannitol also acted as an effective impermeant osmotic agent and prevented most of the cellular swelling which was otherwise seen in response to renal ischemia.

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Variations in fine structure of renal tubular epithelium under different conditions of fixation

Cited by 79 publications

References 14 publications

The cell web in epithelial cells of the rat kidney

The cell web in epithelial cells of the rat kidney

Chronic Intoxication by Cadmium Experimentally Induced in Rabbits

Tandem Scanning Confocal Microscopy (TSCM) of normal and ischemic living kidneys

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