Variations in chondrogenesis of human bone marrow-derived mesenchymal stem cells in fibrin/alginate blended hydrogels

Ma, Kun; Titan, Ashley L.; Stafford, Melissa; Zheng, Chun hua; Levenston, Marc E.

doi:10.1016/j.actbio.2012.06.028

Cited by 58 publications

(40 citation statements)

References 54 publications

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“…Here, the initial cell attachment and proliferation of BMSCs was markedly improved with the introduction of PRP into the SA hydrogel. It is assumed that the composition of PRP in the hydrogel resulted in the incorporation of growth factors and adhesive proteins, like fibronectin and fibrinogen, and thus promoted cell adhesion and cell metabolic activity especially in the early stages of culture (Jooybar et al, ; Ma et al, ). This result is consistent with previous findings showing that PRP has the ability to improve, for instance, cell proliferation (Boyd et al, ; Lee, ).…”

Section: Discussionmentioning

confidence: 99%

“…Hence, platelets can be considered as a potential source of bio‐active factors for cartilage regeneration (Jooybar et al, ; Giraldo, Álvarez, Samudio, Prades, & Carmona, ). Additionally, during the activation of PRP, coagulation can be triggered leading to polymerization of fibrinogen with formation of fibrin, which is an appealing source of natural polymer as component of biomaterials or employed alone as 3D scaffold for tissue engineering (Lee, ; Ma, Titan, Stafford, Zheng, & Levenston, ; Xie et al, ). Activated PRP has been also used either as medium supplement to promote chondrogenic differentiation of marrow stromal cells (MSCs) encapsulated in a 3D alginate scaffold (Beigi et al, ) or incorporated into alginate beads for promoting osteogenic (Fernandes et al, ) or chondrogenic differentiation (Tang et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Gao

Groth

et al. 2019

J Biomedical Materials Res

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Three‐dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue. In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet‐rich‐plasma (PRP) varying in blending ratios, cross‐linked with calcium ions, released from calcium carbonate‐D‐Glucono‐d‐lactone (CaCO3‐GDL) was successfully prepared. It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio. The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure. Field emission scanning electron microscopy (FE‐SEM) investigation confirmed the incorporation of PRP‐derived proteins in the hydrogel, where a porous structure with a pore size of 200–300 μm was found. On the other hand, an increase in surface roughness was observed after the addition of PRP. The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel. The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa. It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP. In terms of cellular responses, all gels exhibited an excellent cytocompatibility. Indeed, the composite hydrogels supported bone marrow‐derived mesenchymal stem cells proliferation and their chondrogenesis with up‐regulation of chondrogenic marker genes Sox9 and Aggrecan. Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Gao

Groth

et al. 2019

J Biomedical Materials Res

View full text Add to dashboard Cite

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“…They can be derived from natural materials which are either components of, or have macro-molecular properties similar to, native extra-cellular matrix [17], and a number of naturally derived hydrogels have been shown to support chondrogenesis of MSCs in vitro [18][19][20][21][22]. Previous studies have compared the chondrogenic capabilities of MSC-seeded hydrogels in vitro [23][24][25][26], and also the potential of chondrogenically primed MSC-seeded hydrogels to maintain a stable chondrogenic phenotype in vivo [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels

et al. 2015

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Cartilaginous tissues engineered using mesenchymal stem cells (MSCs) have been shown to generate bone in vivo by executing an endochondral program. This may hinder the use of MSCs for articular cartilage regeneration, but opens the possibility of using engineered cartilaginous tissues for large bone defect repair. Hydrogels may be an attractive tool in the scaling-up of such tissue engineered grafts for endochondral bone regeneration. In this study, we compared the capacity of different naturally derived hydrogels (alginate, chitosan, and fibrin) to support chondrogenesis and hypertrophy of MSCs in vitro and endochondral ossification in vivo. In vitro, alginate and chitosan constructs accumulated the highest levels of sGAG, with chitosan constructs synthesising the highest levels of collagen. Alginate and fibrin constructs supported the greatest degree of calcium accumulation, though only fibrin constructs calcified homogenously. In vivo, chitosan constructs facilitated neither vascularization nor endochondral ossification, and also retained the greatest amount of sGAG, suggesting it to be a more suitable material for the engineering of articular cartilage.Both alginate and fibrin constructs facilitated vascularization and endochondral bone formation as well as the development of a bone marrow environment. Alginate constructs accumulated significantly more mineral and supported greater bone formation in central regions of the engineered tissue. In conclusion, this study demonstrates the capacity of chitosan hydrogels to promote and better maintain a chondrogenic phenotype in MSCs and highlights the potential of utilizing alginate hydrogels for MSC-based endochondral bone tissue engineering applications.

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“…Cloyd et al, 2007;Gruber et al, 2006;Gupta et al, 2014;Hunt and Grover, 2010;Lazebnik et al, 2011;Nerurkar et al, 2010;Tilwani et al, 2012;Zhang et al, 2012 Fibrin Human plasma Surgical sealant Used as a singular or hybrid scaffold for NP and AF in vitro and in vivo testing. Acosta et al, 2011;Ahmed et al, 2008;Colombini et al, 2014;Diederichs et al, 2012;Eyrich et al, 2007;Hegewald et al, 2010;Ho et al, 2010;Likhitpanichkul et al, 2014;Ma et al, 2012;Park et al, 2011b;Pirvu et al, 2015;Schek et al, 2011;Stern et al, 2000;Stern et al, 2004;Sung et al, 1999;…”

Section: Culture Mediamentioning

confidence: 99%

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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Variations in chondrogenesis of human bone marrow-derived mesenchymal stem cells in fibrin/alginate blended hydrogels

Cited by 58 publications

References 54 publications

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

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