Variations in Blood Parameters of High Salt Loaded Rats Following Administration of Moringa oleifera Leaf Extract

Ofem, OE; Ani, E. J.; Archibong, A. N.; Ufford, J.M.

doi:10.3923/tmr.2015.97.105

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…These findings might be due to the presence of vitamins, and iron in Aloe vera. A similar finding was observed in a study conducted by Ani et al 18 and Ofem et al 20 Group C demonstrated a significant increase in the mean MCH to nearly 18 pg during the same period indicating that Aloe vera helped improve the haemoglobin concentration in PHZ-treated rats. Kolawole and associates 22 showed that the MCH level was significantly decreased in the PHZ-treated only group than that in the normal control group which is consistent with the findings of the present study.…”

Section: Discussionsupporting

confidence: 87%

Effects of Aloe Vera Gel on Red Blood Cell-parameters in Phenylhydrazine-induced Anaemic Wistar albino Rats

Akhter,

Begum,

Jahan

et al. 2024

Ibrahim card. med. j.

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Background & objective: Anaemia is the most significant disorder of the blood. Many researchers have developed the concept of using medicinal plants as an alternative source of treatment for anaemia with fewer side effects. Aloe vera, being a medicinal plant, is popularly used as a natural drug in haematological disorders. The present study was intended to observe the effects of Aloe vera gel on red blood cell parameters in phenylhydrazine (PHZ)-induced anaemic Wistar albino rats. Methods: This Experimental study was conducted in Dhaka Medical College, Dhaka from July 2021 to June 2022. A total of 24 healthy Wistar albino rats were selected for the study. After procurement, the rats were initially kept in a standard laboratory condition on a 12/12-hour light/dark cycle for 14 days of acclimatization before 21 days of the experiment. All the rats had free access to basal diet and normal saline during the period of acclimatization and experiment. After acclimatization for 14 days, the rats were divided into three groups-Group A (baseline control group, n = 8), Group B (PHZ-treated group, n = 8), and Group C, the Experimental Group (PHZ-induced and Aloe vera gel treated group, n = 8). To induce anaemia, Group B and Group C received intraperitoneal injection of PHZ at a dose of 0.5 ml/100 g body weight on days 1, 3, 5 and day 7. Moreover, the experimental group (Group C) received Aloe vera gel orally at a dose of 0.6 ml/kg body weight from Day 8 to Day 21. On day 22, rats were sacrificed to test their blood samples for haematologic variables (RBC count, Hb conc., and PCV) and haematological indices (MCV, MCH and MCHC). Result: All the haematological parameters and indices were almost similar among the study groups at baseline (on Day 1). On Day 8, rats of groups B and C demonstrated a drastically reduced count of RBC (2.66 ± 0.53 and 2.69 ± 0.52 million/μl respectively) compared to the rats of group A (8.16 ± 0.76 million/μl). On Day 22, the RBC count in rats of Group C staggeringly increased to 7.63 ± 0.64 million/μl; however, the RBC count in rats of Group B remained almost the same as was found on Day 8. A significant decrease in Hb conc. and PCV in PHZ-induced control on Day 8 compared to that on Day 1 was also observed. The mean MCV in all the study groups on Day 1 was around 50 fl, which abnormally increased to 85 and 87 fl in Groups B and C respectively on Day 8. On Day 22, Group B further exhibited an increase in MCV to > 87 fl. Meanwhile, the MCV in Group C (Aloe vera-treated group) favourably decreased to 55 fl. On the 8th Day of intervention, the MCH level significantly decreased in Groups B and C. Although no significant change in MCH was observed in Group B from Day 8 to Day 22, group C demonstrated a significant increase in the mean MCH to nearly 18 pg during the same period. The mean MCHC significantly dropped to < 23% in Groups B and C on Day 8 from the baseline figure of 34%. While no significant change in MCHC was evident in Group B from Day 8 to Day 22, it significantly improved to about 28% in Group C during the same period. Conclusion: From the study, it can be concluded that Aloe vera has significant erythropoietic potentials which result in the improvement of red blood cell parameters (RBC count, Hb conc., PCV, MCV, MCH and MCHC) in PHZ-induced anaemic rats. Ibrahim Card Med J 2023; 13 (1&2): 40-46

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Section: Discussionsupporting

confidence: 87%

Effects of Aloe Vera Gel on Red Blood Cell-parameters in Phenylhydrazine-induced Anaemic Wistar albino Rats

Akhter,

Begum,

Jahan

et al. 2024

Ibrahim card. med. j.

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“…However, it provides a cardioprotective effect by reducing nocturnal heart rate increasing ejection volume and cardiac output ( Randriamboavonjy et al, 2016 ). Another study by Ofem et al (2015) reported that aqueous MO leaf extracts might resolve the abnormalities in hematology parameters in rats treated with a high-salt diet such as white blood cells, red blood cells, platelet counts, packed cell volume, lymphocytes, platelet large cell ratio, mean platelet volume, and platelet distribution width (PDW).…”

Section: Resultsmentioning

confidence: 99%

Moringa Oleifera Lam. in Cardiometabolic Disorders: A Systematic Review of Recent Studies and Possible Mechanism of Actions

2022

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Cardiometabolic disorders (CMD) have become a global emergency and increasing burden on health and economic problems. Due to the increasing need for new drugs for cardiometabolic diseases, many alternative medicines from plants have been considered and studied. Moringa oleifera Lam. (MO), one of the native plants from several Asian countries, has been used empirically by people for various kinds of illnesses. In the present systematic review, we aimed to investigate the recent studies of MO in CMD and its possible mechanism of action. We systematically searched from three databases and summarized the data. This review includes a total of 108 papers in nonclinical studies and clinical trials of MO in cardiometabolic-related disorders. Moringa oleifera, extracts or isolated compound, exerts its effect on CMD through its antioxidative, anti-inflammatory actions resulting in the modulation in glucose and lipid metabolism and the preservation of target organ damage. Several studies supported the beneficial effect of MO in regulating the gut microbiome, which generates the diversity of gut microbiota and reduces the number of harmful bacteria in the caecum. Molecular actions that have been studied include the suppression of NF-kB translocation, upregulation of the Nrf2/Keap1 pathway, stimulation of total antioxidant capacity by reducing PKCζ activation, and inhibiting the Nox4 protein expression and several other proposed mechanisms. The present review found substantial evidence supporting the potential benefits of Moringa oleifera in cardiovascular or metabolic disorders.

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“…Similar results have been reported for anemic human patients by Suzana et al,. (2017) and for albino rabbits fed Moringa Ofem et al, (2015). The RDWc is a range of volume and size of RBCs and is a valid indicator of anisocytosis of RBCs.…”

Section: Discussionmentioning

confidence: 99%

Effect of Moringa Oleifera Leaves on Hematological and Serum Chemistry Attributes of Apparently Healthy Rabbit Does

2022

THE JAPS

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The study was designed to assess the effect of Moringa oleifera leaf extract on various hematological and serum chemistry attributes (liver and kidney function test enzymes) of apparently healthy rabbit does (n=24), either positive or negative. The animals were divided in four groups namely A, B, C and D (n=6 rabbits/group). First group was considered as control group provided with basal diet and water without any supplementation. Group A, B and C were considered as treatment groups which were provided with basal diet, water and supplementation of M. oleifera crude leaf aqueous extract at the dose rates of 150mg/kg, 300mg/kg, and 450 mg/kg, respectively. On day 10, 20, 30 and 40, blood samples were collected from all animals. The blood samples (n=96) were analyzed through hematology analyzer and semi-automatic chemistry analyzer. Results regarding hematological attributes revealed that the Mitotic Index was significantly (P≤0.05) higher in control group whereas Red Cell Distribution Width Count was significantly (P≤0.05) higher in Group C as compared to their counterpart groups. Similarly, Total Erythrocytic Count was significantly (P≤0.05) higher in Group B. The creatinine, urea and blood urea nitrogen differed significantly (P≤0.05) while Uric acid showed non-significant (P≥0.05) difference between the groups. It is concluded that varying doses of Moringa do not exert any untoward effect on hematochemical attributes of rabbits and hence their health. Rather, positive effects were noticed at dose rate of 150mg/kg.

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Variations in Blood Parameters of High Salt Loaded Rats Following Administration of Moringa oleifera Leaf Extract

Cited by 6 publications

References 20 publications

Effects of Aloe Vera Gel on Red Blood Cell-parameters in Phenylhydrazine-induced Anaemic Wistar albino Rats

Effects of Aloe Vera Gel on Red Blood Cell-parameters in Phenylhydrazine-induced Anaemic Wistar albino Rats

Moringa Oleifera Lam. in Cardiometabolic Disorders: A Systematic Review of Recent Studies and Possible Mechanism of Actions

Effect of Moringa Oleifera Leaves on Hematological and Serum Chemistry Attributes of Apparently Healthy Rabbit Does

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