Variations for red rot resistance in somaclones of sugarcane

Singh, Atul Pratap; Lai, Mingtan; Singh, Madhulika; Lai, Kwan-Long; Singh, S. B.

doi:10.1007/bf02945746

Cited by 16 publications

(14 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stressors, choice of their concentration and preparation of samples were based on guidelines in the publication [16]. As the drug was insoluble in water, it was dissolved in a mixture of acetonitrile and potassium dihydrogen ortho phosphate buffer (pH 5.0; 0.01M) in a ratio of 50:50 (v/v) to a final concentration of 2 mg/ml.…”

Section: Stress Decomposition Studiesmentioning

confidence: 99%

A validated stability indicating RP-HPLC method for valganciclovir, identification and characterization of forced degradation products of valganciclovir using LC-MS/MS

Sawant¹,

Barge²

2014

Acta Chromatographica

View full text Add to dashboard Cite

Summary. The objective of the present study was to report the stability of novel antiviral drug, valganciclovir based on the information obtained from forced degradation studies. Valganciclovir was subjected to forced hydrolytic (acidic, alkaline and neutral), oxidative, photolytic and thermal stress in accordance with the ICH guideline Q1A (R 2 ). The drug showed labiality under only acidic and photoacidic conditions while it was stable to other stress conditions. Resolution of the drug and degradation products was achieved on a Hypersil Gold C-18 column (4.6 × 250 mm, 5 μm) utilizing acetonitrile (A) and potassium dihydrogen ortho phosphate buffer (pH 5.0; 0.01M) in the ratio of 5:95 (v/v) at a flow rate of 0.6 ml/min and at the detection wavelength 252 nm. The major acidic stress degradation product was characterized by LC-MS/MS and its fragmentation pathway was proposed. Validation of the LC-DAD method was carried out in accordance with ICH guideline. The method met all required criteria and was applied for analysis of commercially available tablets.

show abstract

Section: Stress Decomposition Studiesmentioning

confidence: 99%

A validated stability indicating RP-HPLC method for valganciclovir, identification and characterization of forced degradation products of valganciclovir using LC-MS/MS

Sawant¹,

Barge²

2014

Acta Chromatographica

View full text Add to dashboard Cite

show abstract

“…The major advantage of HPTLC is that several samples can be run simultaneously using a small quantity of mobile phase unlike HPLC, thus reducing the analysis time and cost per analysis [10,11]. Hence, the objective of the present study was to develop a stabilityindicating HPTLC method [12] for estimation of repaglinide as bulk drug and in formulations and to perform stress studies under a variety of ICHrecommended test conditions [1,13]. The proposed method was validated for linearity, accuracy (recovery studies), specificity, precision, robustness, ruggedness, LOD (limit of detection), LOQ (limit of quantitation), and repeatability according to the ICH guidelines [14,15] and its updated international convention [16].…”

Section: S(+)2-ethoxy-4(2((3-methyl-1-(2-(1-piperidinyl)phenyl)-butmentioning

confidence: 99%

Development and validation of stability-indicating high-performance thin-layer chromatography method for estimation of repaglinide in bulk and in pharmaceutical formulation

Jain¹,

Patel²,

Surana³

2013

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A simple, selective, precise, and stability-indicating high-performance thinlayer chromatographic method for analysis of repaglinide both in a bulk and in pharmaceutical formulation has been developed and validated. The method employed high-performance thin-layer chromatography (HPTLC) aluminum plates precoated with silica gel 60 RP-18 F 254 as the stationary phase. The solvent system consisted of chloroform-methanol-ammonia (4.5:0.8:0.05, v/v). The system was found to give compact spot for repaglinide (R F value of 0.55 ± 0.03). Densitometric analysis of repaglinide was carried out in the absorbance mode at 288 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r 2 = 0.998 ± 0.0015 with respect to peak area in the concentration range 600-1600 ng per spot. The mean value ± SD of slope and intercept were 3.38 ± 1.47 and 986.9 ± 108.78, with respect to peak area. The method was validated for precision, recovery, and robustness. The limits of detection and quantification were 22.64 and 68.84 ng per spot, respectively. Repaglinide was subjected to acid and alkali hydrolysis, oxidation, and thermal degradation. The drug undergoes degradation under acidic and basic conditions. This indicates that the drug is susceptible to acid and base. The degraded product was well resolved from the pure drug with significantly different R F value. Statistical analysis proves that the method is repeatable, selective, and accurate for the estimation of investigated drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of repaglinide in bulk drug and pharmaceutical formulation.

show abstract

“…The specificity of the developed LC method for bortezomib is determined in the presence of its related impurities (namely imp-1, imp-2, imp-3, imp-4 and imp-5) and degradation products. Forced degradation studies are also performed on bortezomib to provide an indication of the stability-indicating property and specificity of the proposed method [12][13][14][15][16][17]. The stress conditions employed for the degradation study included light (carried out as per ICH Q1B), heat (105˚C), acid hydrolysis (0.2 M HCl), base hydrolysis (0.2 M NaOH) and oxidation (25% H 2 O 2 ).…”

Section: Stress Studies / Specificitymentioning

confidence: 99%

A Validated Stability-Indicating UF LC Method for Bortezomib in the Presence of Degradation Products and its Process-Related Impurities

Madireddy¹,

Babu²,

Anwar³

2012

J Chromatograph Separat Techniq

View full text Add to dashboard Cite

Degradation pathway for bortezomib is established as per ICH recommendations by validated and stability indicating reverse phase liquid chromatographic method. Bortezomib is subjected to stress conditions of acid, base, oxidation, thermal and photolysis. Significant degradation is observed in acid and base stress conditions. Five impurities are studied and the major degradant (RRT about 1.19) was identified by LC-MS and spectral analysis. The stress samples are assayed against a qualified reference standard and the mass balance is found close to 99.5 %. Efficient chromatographic separation is achieved on a Shim pack XR-ODS-II(100X3mmx2.2µm) stationary phase with simple mobile phase combination delivered in gradient mode and quantification is carried at 270 nm at a flow rate of 0.6 mL min -1 . In the developed LC method the resolution between bortezomib and five potential impurities (imp-1, imp-2, imp-3, imp-4 and imp-5) is found to be greater than 2.0. Regression analysis shows an r value (correlation coefficient) of greater than 0.999 for bortezomib and five potential impurities. This method is capable to detect the impurities of bortezomib at a level of 0.020 % with respect to test concentration of 2.0 mg mL -1 for an 8-µL injection volume. The developed rapid LC method is validated with respect to specificity, linearity & range, accuracy, precision and robustness for impurities determination.

show abstract

Variations for red rot resistance in somaclones of sugarcane

Cited by 16 publications

References 1 publication

A validated stability indicating RP-HPLC method for valganciclovir, identification and characterization of forced degradation products of valganciclovir using LC-MS/MS

A validated stability indicating RP-HPLC method for valganciclovir, identification and characterization of forced degradation products of valganciclovir using LC-MS/MS

Development and validation of stability-indicating high-performance thin-layer chromatography method for estimation of repaglinide in bulk and in pharmaceutical formulation

A Validated Stability-Indicating UF LC Method for Bortezomib in the Presence of Degradation Products and its Process-Related Impurities

Contact Info

Product

Resources

About