Variations and heredity in bacterial colonies

Čepl, Jaroslav; Blahůšková, Anna; Neubauer, Zdeněk; Markoš, Anton

doi:10.1080/19420889.2016.1261228

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…It is expected that the number of colonies formed is linearly proportional to the concentration of viable cells in the suspension, provided that the suspension was mixed and spread well. If the suspension is not spread well, clusters of cells will be formed which visually resemble a single colony and therefore will be enumerated as a single colony [8]; furthermore, bias may be introduced when analyzing genetic variations and heredity of such clusters of cells [9]. To proceed with further procedures, the cells need to be restreaked to get single colonies, which takes extra incubation time.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of glass beads for plating cell cultures

et al. 2021

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Cell plating, the spreading out of a liquid suspension of cells on a surface followed by colony growth, is a common laboratory procedure in microbiology. Despite this, the exact impact of its parameters on colony growth has not been extensively studied. A common protocol involves the shaking of glass beads within a Petri dish containing solid growth media. We investigated the effects of multiple parameters in this protocol: the number of beads, the shape of movement, and the number of movements. Standard suspensions of Escherichia coli were spread while varying these parameters to assess their impact on colony growth. Results were assessed by a variety of metrics: the number of colonies, the mean distance between closest colonies, and the variability and uniformity of their spatial distribution. Finally, we devised a mathematical model of shifting billiard to explain the heterogeneities in the observed spatial patterns. Exploring the parameters that affect the most fundamental techniques in microbiology allows us to better understand their function, giving us the ability to precisely control their outputs for our exact needs.

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Section: Introductionmentioning

confidence: 99%

Effectiveness of glass beads for plating cell cultures

et al. 2021

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“…It is expected that the number of colonies formed is linearly proportional to the concentration of viable cells in the suspension, provided that the suspension was mixed and spread well. If the suspension is not spread well, clusters of cells will be formed which visually resemble a single colony and therefore will be enumerated as a single colony [8]; furthermore bias may be introduced when analysing genetic variations and heredity of such clusters of cells [9]. To proceed with further procedures, the cells need to be restreaked to get single colonies which takes extra incubation time.…”

Section: Introductionmentioning

confidence: 99%

The effectiveness of glass beads for plating cell cultures

Prusokas

Hawkins

Nieduszynski

et al. 2018

Preprint

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Cell plating and subsequent colony growth is an important laboratory procedure in microbiology. A common protocol for cell culture plating in Petri dishes uses glass beads to spread cells on the surface of the solid growth media. Here we perform an experimental analysis and mathematical modelling of cell plating. We introduce a new class of billiard -shifting billiard, which serves as a conceptual abstraction of the cell plating with glass beads. Numerical analysis of the dynamics of this simple yet efficient billiard, indicates a close relationship between plate movement and quality of colony distribution. Yeast Saccharomyces cerevisiae suspensions at different cell concentrations were used to confirm our theoretical observations.

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