Variation of spontaneous somatic mutation frequency in the stamen hairs of Tradescantia clone BNL 02.

Ichikawa, Sadao; Shima, Naoko; Ishii, Chizu; Kanai, Hiromi; Sanda-Kamigawara, Marie; Matsuura‐Endo, Chie

doi:10.1266/ggs.71.159

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…In the literature, this value ranged from 10 to 21 days for chronic exposure to radiation. After this period, the highest values of mutations were found in the analyzed flowers matching with values found in the work of Takahashi & Ichikawa (1976), Ichikawa (1981) and Ichikawa et al (1996). In their dose-effect experiments on exposure to different radiation rates, it was observed that mutations were more frequent when exposed to environmental factors about two or three weeks before flowering.…”

Section: Introductionsupporting

confidence: 74%

Air pollution monitoring with Tradescantia hybrid and optical sensors in Curitiba and Araucária, Brazil

Rodrigues,

Mercuri,

Noe

2023

Forestry Studies

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Complex mixtures of substances are in the atmosphere and they can cause diseases in humans and biological communities after acute or chronic exposition. This paper focuses on the physical measurement of particulate matter, a proxy for air pollution, and a biological method for mutation assessment due to plants’ exposure to air pollution. The objective of this research was to characterize the air pollution seasonality in municipalities in southern Brazil, and also to understand the relation between air pollution and the biological response of the Tradescantia sp. clone 4430. The optical sensor SDS011 was used for measurements of particulate matter (PM) and the Trad-SHM bioassay was chosen to quantify the mutagenic alterations that occurred in stamen hairs during the study period, with PM data being measured every 5 seconds and the flowers being harvested approximately every two weeks for laboratory analysis. The Pearson test was applied to verify the correlation between PM and mutations in stamen hair as a result of which it was observed that there is a positive correlation between these data, with the highest value found being r = 0.61. Also, the period with the highest occurrence of pink cells was between autumn and spring, the same period in which an unusual increase in PM concentrations was also observed, a period that corresponds to a less favorable dispersion of pollutants in the atmosphere. The use of Tradescantia sp. clone 4430 showed sensitivity to the environments in which it was exposed. Biomonitoring is an important tool for understanding the effects of pollutants on the ecosystem.

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Section: Introductionsupporting

confidence: 74%

Air pollution monitoring with Tradescantia hybrid and optical sensors in Curitiba and Araucária, Brazil

Rodrigues,

Mercuri,

Noe

2023

Forestry Studies

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“…With other clones, a negative correlation was found between both variables over the same temperature range (T akahashi and I chikawa 1976). When pooling data from different experiments, “nearly significant correlations” between spontaneous mutation frequency in clone BNL 02 and average temperature were obtained even in a quite narrow temperature range of 20 to 23°C (I chikawa et al 1996). In general, high temperatures proved to have an effect on the spontaneous mutation rates in stamen hairs, however, the kind and intensity of the response depended strongly on the plant material and assay methods applied.…”

Section: Discussionmentioning

confidence: 99%

Influence of climatic conditions on the mutations in pollen mother cells of Tradescantia clone 4430 and implications for the Trad-MCN bioassay protocol

et al. 2004

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The present study was aimed at investigating the influence of relative humidity and temperature on spontaneous and pollution-induced mutation rates during exposure and recovery periods in the Trad-MCN test. Cuttings of Tradescantia clone 4430 were exposed to a negative control, to 4 mM maleic hydrazide (MH), and to a polluted water sample under varying conditions of air temperature and humidity in climatic chamber experiments. The relative humidity did not affect the spontaneous mutation rate in the clone investigated, but was negatively correlated with the frequency of pollution-induced mutations. Low temperature caused an increase in the number of micronuclei in the negative control, but no comparable response in polluted samples. At an extremely high temperature, signs of strong physiological damage and/or of a meiotic delay of pollen maturation were detected. When the temperature increased gradually and the extreme value was maintained only for short time, such detrimental effects were not observed. Subsequent treatment with high and low temperatures, by contrast, resulted in the highest MCN rates of all experiments. Our studies point to the possibility of producing irregular results of the Trad-MCN test if the influence of climatic factors has not sufficiently been considered.

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“…The use of this system makes it possible to collect a large number of samples relatively easily and to detect all pink mutant cells occurred without being concealed by other cells (Ichikawa, 1992). Because of these merits, this system has been successfully used for studying the variation of spontaneous mutation frequency (Sparrow and Sparrow, 1976;Takahashi and Ichikawa, 1976;Mericle et al, 1976;Nauman et al, 1978;Ichikawa et al, 1981Ichikawa et al, , 1996aIchikawa et al, , 1996cIchikawa, 1984;Imai et al, 1991;, as well as for detecting mutagenic synergisms among several chemical mutagens and X rays (CebulskaWasilewska et al, 1981;Ichikawa, 1992;Ichikawa et al, 1993;Shima and Ichikawa, 1994, 1997Xiao and Ichikawa, 1995).…”

Section: Introductionmentioning

confidence: 99%

Effects of X-ray dose fractionations with various intervals in inducing somatic mutations in the stamen hairs of Tradescantia clone KU 9.

Sakuramoto

Ichikawa

1996

Genes Genet. Syst.

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The effects of X-ray dose fractionations in inducing somatic mutations were studied in the stamen hairs of Tradescantia clone KU 9 heterozygous for flower color (blue/pink). Young inflorescence-bearing shoots with roots were prepared, and were exposed to X rays acutely. A dose-response regression line with a slope of 1.454 on a log-log graph was obtained for single acute X-ray doses of 0.255 to 1.03 Gy, and it showed that somatic mutation frequency increased curvilinearly with increasing X-ray dose. When 1.00 to 1.15 Gy of X rays were fractionated into two acute doses of about halves given with intervals of 5 to 120 min, decreases in induced mutation frequency were observed. The mutation frequencies induced by the fractionated doses with intervals of 5 and 10 min were not significantly different from those expected for the total single doses. However, the mutation frequency decreased significantly at 5% level with 20-and 30-min intervals, and decreases were highly significant at 0.1% level when the interval was prolonged to 40 to 120 min. The results obtained indicate that the interaction between the first and second doses began to reduce between 10 and 20 min later, and disappeared by 60 min later. That is, the DNA and/or chromosomal breaks induced by the first dose began to be rejoined (repaired) or healed between 10 and 20 min later, and all of them were rejoined or healed by 60 min later, losing their abilities to interact with the DNA and/or chromosomal breaks induced by the second dose.

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Variation of spontaneous somatic mutation frequency in the stamen hairs of Tradescantia clone BNL 02.

Cited by 7 publications

References 28 publications

Air pollution monitoring with Tradescantia hybrid and optical sensors in Curitiba and Araucária, Brazil

Air pollution monitoring with Tradescantia hybrid and optical sensors in Curitiba and Araucária, Brazil

Influence of climatic conditions on the mutations in pollen mother cells of Tradescantia clone 4430 and implications for the Trad-MCN bioassay protocol

Effects of X-ray dose fractionations with various intervals in inducing somatic mutations in the stamen hairs of Tradescantia clone KU 9.

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