Variation of secondary metabolites in Cannabis sativa L. inflorescences under applied agrotechnological measures

Barčauskaitė, Karolina; Bakšinskaitė, Aušra; Szumny, Antony; Tilvikienė, Vita

doi:10.1016/j.indcrop.2022.115570

Cited by 8 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that despite agrochemical conditions, the dominant acid is cannabidiolic acid, which ranged from 15.43% to 24.99% of the total amount of identified compounds. This result is justified by the fact that the hemp variety Felina 32 is known as a cannabidiol-producing hemp type, and similar results were presented in previous works [14,26]. Two polyunsaturated fatty acids: linoleic acid and alfa-linolenic acid, were also found in significant amounts, averaging around 18.89 and 12.92, respectively.…”

Section: Organic Acids Composition In Hemp Inflorescencesupporting

confidence: 82%

“…Literature analysis confirmed the presence of diverse cannabinoids in hemp plants, such as CBG, CBD, CBC, CBL, CBE, and CBN [35]. Obtained results correspond to different experiments that describe the cannabinoids composition in different hemp parts using methanolic extraction [14,36].…”

Section: Antimicrobial Activitysupporting

confidence: 70%

“…Information on other cannabinoids is lacking because often only CBD, THC, or total cannabinoid levels are studied. Other types of cannabinoids are found in low quantities and are called minor cannabinoids [14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation of Biologically Active Compounds from Cannabis sativa L. Inflorescences by Using Different Extraction Solvents and Evaluation of Antimicrobial Activity

et al. 2023

Self Cite

View full text Add to dashboard Cite

Hemp inflorescences are a source of vital compounds, including phytocannabinoids and other biologically active compounds. Various methods are adapted for the extraction of these vital compounds such as the use of different organic solvents. This study aimed to assess the comparative extraction potential of three different solvents: deionized water, 70% methanol (MeOH), and 2% Triton X-100, for phytochemicals in hemp inflorescences. Spectrophotometric techniques were applied to investigate the total amount of polyphenolic compounds (TPC), total flavonoids contents (TF), phenolic acids (TPA), and radical scavenging activity (RSA) in hemp extracts obtained using different polarity solvents. Gas chromatography-mass spectrometry was used for cannabinoids and organic acids quantitative analysis. In the results, MeOH showed a better affinity for the recovery of TFC, TPA, and RSA in comparison to Triton X-100 and water. However, Triton X-100 performed better for TPC with 4-folds and 33% turnover compared to water and MeOH, respectively. Six cannabinoids (CBDVA, CBL, CBD, CBC, CBN, and CBG) were identified in hemp inflorescence extracts. The maximum determined concentration was as follows: CBD > CBC > CBG > CBDVA > CBL > CBN. Overall, fourteen organic acids were identified. Hemp inflorescence extracts obtained using 2% Triton X-100 showed an effect on all tested strains of microorganisms. Methanolic and aqueous extracts had antimicrobial activity against seven tested strains. On the other hand, the inhibition zones were wider for methanolic extracts compared to aqueous ones. Hemp aqua extract with antimicrobial activity might be used in various markets where toxic solvents are unwanted.

show abstract

Section: Organic Acids Composition In Hemp Inflorescencesupporting

confidence: 82%

Section: Antimicrobial Activitysupporting

confidence: 70%

See 1 more Smart Citation

Isolation of Biologically Active Compounds from Cannabis sativa L. Inflorescences by Using Different Extraction Solvents and Evaluation of Antimicrobial Activity

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…The additional cyclization or substitution of additional groups may produce different cannabinoid isomers. As with Δ 9 -THC, a cannabinoid that is primarily responsible for the psychoactive effects experienced after marijuana use, Δ 8 -THC is also naturally occurring (albeit at a much lower concentration) in the plant 6 . Understanding of the molecular targets, bioactivity, and analytical methods to characterize minor cannabinoids, such as Δ 8 -THC, and impurities from their synthesis is a developing area of science and presents a research gap 7 .…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a GC-FID Method for the Quantitation of Δ 8-Tetrahydrocannabinol and Impurities Found in Synthetic Δ 8-Tetrahydrocannabinol and Vaping Products

Gul,

Shahzadi,

Sarma

et al. 2024

Planta Med

View full text Add to dashboard Cite

Concerns about health hazards associated with the consumption of trans-delta-8-tetrahydrocannabinol products were highlighted in public health advisories from the U. S. Food and Drug Administration and U. S. Centers for Disease Control and Prevention. Simple and rapid quantitative methods to determine trans-delta-8-tetrahydrocannabinol impurities are vital to analyze such products. In this study, a gas chromatography-flame ionization detection method was developed and validated for the determination of delta-8-tetrahydrocannabinol and some of its impurities (recently published) found in synthesized trans-delta-8-tetrahydrocannabinol raw material and included olivetol, cannabicitran, Δ 8-cis-iso-tetrahydrocannabinol, Δ 4-iso-tetrahydrocannabinol, iso-tetrahydrocannabifuran, cannabidiol, Δ 4,8-iso-tetrahydrocannabinol, Δ 8-iso-tetrahydrocannabinol, 4,8-epoxy-iso-tetrahydrocannabinol, trans-Δ 9-tetrahydrocannabinol, 8-hydroxy-iso-THC, 9α-hydroxyhexahydrocannabinol, and 9β-hydroxyhexahydrocannabinol. Validation of the method was assessed according to the International Council for Harmonization guidelines and confirmed linearity with R2 ≥ 0.99 for all the target analytes. The limit of detection and limit of quantitation were 1.5 and 5 µg/mL, respectively, except for olivetol, which had a limit of detection of 3 µg/mL and a limit of quantitation of 10 µg/mL. Method precision was calculated as % relative standard deviation and the values were less than 8.4 and 9.9% for the intraday precision and inter-day precision, respectively. The accuracy ranged from 85 to 118%. The method was then applied to the analysis of 21 commercially marketed vaping products claiming to contain delta-8-tetrahydrocannabinol. The products analyzed by this method have various levels of these impurities, with all products far exceeding the 0.3% of trans-Δ 9-tetrahydrocannabinol limit for hemp under the Agriculture Improvement Act of 2018. The developed gas chromatography-flame ionization detection method can be an important tool for monitoring delta-8-tetrahydrocannabinol impurities in commercial products.

show abstract

“…Furthermore, management strategies can be used to induce a rise in secondary metabolites (Adesina et al, 2020;Kakabouki et al, 2021). Thus, cultivating medicinal plants for secondary metabolite extraction necessitate knowledge of genotype responses under different conditions, as the abiotic factors considered ideal for plant development may not be the same ones that promote an increase in secondary metabolites syntactically (Barčauskaite et al, 2022). It is crucial to note that, under stress-induced conditions, the biosynthetic pathway can be accelerated, resulting in an increase in the plant's production of these compounds (Hawley et al, 2018;Kakabouki et al, 2021;Barčauskaite et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Effect of Topping on Growth Development and CBD Content of Hemp (Cannabis sativa L.) in Pot Culture

ROUSSIS,

BILALIS,

PAPASTYLIANOU

et al. 2022

BUASVMCN-HORT

View full text Add to dashboard Cite

In hemp (Cannabis sativa L.) cultivation, an important management factor is apical cut (topping) as this modulates plant architecture, plant biomass allocation as well as the yield of inflorescences and cannabinoids per plant and area. This study aimed to evaluate the effect of topping treatment on agronomical and quality characteristics of two monoecious hemp varieties, ‘Fedora 17’ and ‘Felina’, A greenhouse pot experiment was laid out in a completely randomized design (CRD), with two topping treatments (untreated and topped). The results revealed that plant height of both varieties was negatively affected by topping treatment and the higher values were recorded in the untreated (137.02 and 134.56 cm for Fedora 17 and Felina, respectively). The highest values of dry weight per plant and bud dry weight per plant were noticed for topped plants. In conclusion, the findings of the present study implied that the hemp had a positive response to applied topping for all studied cultivars and the CBD content was higher in topped plants as topping stress increased cannabidiol (CBD) content.

show abstract

Variation of secondary metabolites in Cannabis sativa L. inflorescences under applied agrotechnological measures

Cited by 8 publications

References 26 publications

Isolation of Biologically Active Compounds from Cannabis sativa L. Inflorescences by Using Different Extraction Solvents and Evaluation of Antimicrobial Activity

Isolation of Biologically Active Compounds from Cannabis sativa L. Inflorescences by Using Different Extraction Solvents and Evaluation of Antimicrobial Activity

Development and Validation of a GC-FID Method for the Quantitation of Δ 8-Tetrahydrocannabinol and Impurities Found in Synthetic Δ 8-Tetrahydrocannabinol and Vaping Products

Effect of Topping on Growth Development and CBD Content of Hemp (Cannabis sativa L.) in Pot Culture

Contact Info

Product

Resources

About