Variation of pro‐vasopressin processing in parvocellular and magnocellular neurons in the paraventricular nucleus of the hypothalamus: Evidence from the vasopressin‐related glycopeptide copeptin

Kawakami, Natsuko; Otubo, Akito; Maejima, Sho; Talukder, Ashraf Hossain; Satoh, Keita; Oti, Takumi; Takanami, Keiko; Ueda, Yasumasa; Itoi, Keiichi; Morris, John F.; Sakamoto, Tatsuya; Sakamoto, Hirotaka

doi:10.1002/cne.25026

Cited by 14 publications

(14 citation statements)

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“…It is identical in rats [40], mice [41], macaque monkeys [14], and humans [40]. The copeptin antiserum has also been characterized previously by our immunohistochemistry [22], Western [23] and dot blot [22] analyses. The rabbit polyclonal antiserum against rat/human CRF (PBL rC70), which was kindly donated by Wylie Vale, has been characterized previously [14,22,24].…”

Section: Immunofluorescencementioning

confidence: 78%

“…Western blot analysis demonstrated the specificity for the guinea pig polyclonal antibody against VGLUT2 and the expression of VGLUT2 at the protein level in the posterior pituitary; a single strong immunoreactive band was detected at the expected molecular weight of~56 kDa [21] (Figure 1). The mouse monoclonal antibody against AVP-neurophysin II (NPII) also detected a single major band at the expected molecular weight of~12 kDa on the blots of posterior pituitary [14,22] (Figure 1).…”

Section: Antibody Characterization and The Expression Of Vglut2 At The Protein Level In The Posterior Pituitarymentioning

confidence: 99%

“…The copeptin antiserum has also been characterized previously by our immunohistochemistry [22], Western [23] and dot blot [22] analyses. The rabbit polyclonal antiserum against rat/human CRF (PBL rC70), which was kindly donated by Wylie Vale, has been characterized previously [14,22,24]. It was directed against the rat form of CRF, which is identical to the human form, and has also been characterized in non-human primates [14,25].…”

Section: Immunofluorescencementioning

confidence: 99%

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Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at −25 °C for Several Years

Otubo

Maejima

Oti

et al. 2021

IJMS

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Translational research often requires the testing of experimental therapies in primates, but research in non-human primates is now stringently controlled by law around the world. Tissues fixed in formaldehyde without glutaraldehyde have been thought to be inappropriate for use in electron microscopic analysis, particularly those of the brain. Here we report the immunoelectron microscopic characterization of arginine vasopressin (AVP)-producing neurons in macaque hypothalamo-pituitary axis tissues fixed by perfusion with 4% formaldehyde and stored at –25 °C for several years (4–6 years). The size difference of dense-cored vesicles between magnocellular and parvocellular AVP neurons was detectable in their cell bodies and perivascular nerve endings located, respectively, in the posterior pituitary and median eminence. Furthermore, glutamate and the vesicular glutamate transporter 2 could be colocalized with AVP in perivascular nerve endings of both the posterior pituitary and the external layer of the median eminence, suggesting that both magnocellular and parvocellular AVP neurons are glutamatergic in primates. Both ultrastructure and immunoreactivity can therefore be sufficiently preserved in macaque brain tissues stored long-term, initially for light microscopy. Taken together, these results suggest that this methodology could be applied to the human post-mortem brain and be very useful in translational research.

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Section: Immunofluorescencementioning

confidence: 78%

Section: Antibody Characterization and The Expression Of Vglut2 At The Protein Level In The Posterior Pituitarymentioning

confidence: 99%

Section: Immunofluorescencementioning

confidence: 99%

See 1 more Smart Citation

Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at −25 °C for Several Years

Otubo

Maejima

Oti

et al. 2021

IJMS

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“…This pattern of labeling in which OXT and AVP cells mainly consist of single expression is also observed at the different developmental stages ( Figure S2 ). Here, we used AVP-NPII-immunofluorescence labeling as a marker for the AVP neuronal population, as AVP-associated Neurophysin II and mature AVP are co-expressed by the same cells ( Figure S3 ), as it was previously described in another experimental condition ( Ben-Barak et al., 1985 ; Castel and Morris, 1988 ; Kawakami et al., 2021 ). Separate quantitative analysis also revealed a similar number of cells immunoreactive for either AVP or AVP-NPII in the hypothalamus at birth and in the adult ( Figure S3 ).…”

Section: Resultsmentioning

confidence: 98%

Differential fate between oxytocin and vasopressin cells in the developing mouse brain

Soumier¹,

Habart²,

Lio³

et al. 2022

iScience

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Summary Oxytocin (OXT) and arginine vasopressin (AVP), two neuropeptides involved in socio-emotional behaviors have been anatomically defined in the adult brain. Yet their spatial organization during postnatal development is not clearly defined. We built a developmental atlas using 3D imaging of cleared immunolabeled tissue over four early postnatal (P) stages, from birth (P0, P3, P7, P14) to young adulthood (≥P56). Our atlas-based mapping revealed that the number of OXT neurons doubles according to unique temporal dynamics in selective hypothalamic regions, namely, the periventricular and paraventricular nuclei, and in a novel location we named the antero-lateral preoptic. In the paraventricular nucleus, single-cell densities and fluorescence analysis demonstrated selective expansion of OXT cells in the antero-ventral division, whereas the postero-dorsal division contained cells present at birth. No changes were observed for AVP neurons. Our findings show the coexisting of innate and plastic OXT/AVP brain circuits probably triggered by environmental adaptation of the social brain.

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“…Both of these cell groups are found within the preoptic area, an ancestral common region of VP production which gave rise to separate disparate populations in anamniotes (Goodson and Kabelik, 2009). In amniotes, both magnocellular and parvocellular VP neurons are present in the PVN (Kabelik et al, 2008c;Kawakami et al, 2021;Panzica et al, 1999), and this heterogeneity of cell types with separate functions may be one reason for the different functions and behavioral relationships attributed to VP neurons of the PVN across studies.…”

Section: Ties To Vp Research In Other Vertebrate Taxamentioning

confidence: 99%

Aggressive but not reproductive boldness in male green anole lizards correlates with baseline vasopressin activity

Kabelik¹,

Julien²,

Waddell³

et al. 2021

Preprint

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Across species, individuals within a population differ in their level of boldness in social encounters with conspecifics. This boldness phenotype is often stable across both time and social context (e.g., reproductive versus agonistic encounters). Various neural and hormonal mechanisms have been suggested as underlying these stable phenotypic differences, which are often also described as syndromes, personalities, and coping styles. Most studies examining the neuroendocrine mechanisms associated with behavioral boldness examine subjects after they have engaged in a social interaction, whereas baseline neural activity that may predispose behavioral variation is understudied. The present study tests the hypotheses that physical characteristics, steroid hormone levels, and baseline variation in Ile3-vasopressin (VP, a.k.a., Arg8-vasotocin) signaling predispose social boldness. Behavioral boldness in agonistic and reproductive contexts was extensively quantified in male green anole lizards (Anolis carolinensis), an established research organism for social behavior research that provides a crucial comparison group to investigations of birds and mammals. We found high stability of boldness across time, and between agonistic and reproductive contexts. Next, immunofluorescence was used to colocalize VP neurons with phosphorylated ribosomal protein S6 (pS6), a proxy marker of neural activity. VP-pS6 colocalization within the paraventricular and supraoptic nuclei of the hypothalamus was inversely correlated with aggression boldness, but not reproductive behavior boldness. Our findings suggest that baseline vasopressin release, rather than solely context-dependent release, plays a role in predisposing individuals toward stable levels of aggressive boldness toward conspecifics by inhibiting behavioral output in these contexts.

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Variation of pro‐vasopressin processing in parvocellular and magnocellular neurons in the paraventricular nucleus of the hypothalamus: Evidence from the vasopressin‐related glycopeptide copeptin

Cited by 14 publications

References 58 publications

Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at −25 °C for Several Years

Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at −25 °C for Several Years

Differential fate between oxytocin and vasopressin cells in the developing mouse brain

Aggressive but not reproductive boldness in male green anole lizards correlates with baseline vasopressin activity

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