Variation of Photosynthesis, Fatty Acid Composition, ATPase and Acid Phosphatase Activities, and Anatomical Structure of Two Tea (<i>Camellia sinensis</i> (L.) O. Kuntze) Cultivars in Response to Fluoride

Wang, L. X.; Tang, Juhong; Xiao, Bin; Yang, Yalin; Liu, J.

doi:10.1155/2013/109367

Cited by 5 publications

(8 citation statements)

References 42 publications

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“…The young leaves, stems, and roots of the seedlings were collected after 1 day of treatment at 0 and 0.3 mM NaF for expression analysis and after 14 days of treatment for fluoride measurement. The different tendency to fluoride accumulation in the four tea cultivars under the condition is consistent with the previous studies, as indicated in Table S1 …”

Section: Materials and Methodssupporting

confidence: 91%

“…The different tendency to fluoride accumulation in the four tea cultivars under the condition is consistent with the previous studies, as indicated in Table S1. 27 Cloning of the CsFEX Gene. Putative CsFEX ORF (CSA014827) was amplified with PrimeSTAR Max DNA polymerase (TAKARA) from cDNA extracted from leaves of Yunkang 10 and then cloned into the T Blunt vector.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…It has been well known that the tea plant is a fluoride hyperaccumulator, which is affected by several factors, including brewing process, , soil conditions, seasonal changes, tea leaf growth, and tea cultivars. , Several studies have investigated the distribution of fluoride accumulation in tea plants and revealed that most of the fluoride is accumulated in tea leaves rather than other tissues and the vacuoles contain more than 90% of the total fluoride measured in tea leaves. ,− In addition, the fluoride contents in tea leaves vary significantly among different cultivars in diverse cultivation areas. ,, Because the total fluoride in soil profiles have been reported to vary from 10- to 2000-fold in the same area, even the same cultivar grown in different sites exhibits altered fluoride content in leaves significantly . For example, the fluoride contents of four cultivars Zhe-nong 113, Zhe-nong 121, Zi-sun, and Bi-feng grown at two tea plantations were measured.…”

Section: Introductionmentioning

confidence: 99%

“…Fluoride accumulation in the tea plant involves the processes of fluoride uptake, redistribution, and export,which are associated with the membrane potential gradient, metabolic energy, and transporter proteins. , Fluoride is usually stored by cell wall fixation, vacuole compartmentalization, and metal ion chelation. − A major advance in understanding fluoride accumulation came with the recent discovery of two types of fluoride transporters in prokaryotes and eukaryotes. The first is a unique subclass, named as CLC F in the CLC anion-transporting protein superfamily that function as an antiporter or channel in bacteria and plants to resist halogen ion toxicity from the environment. , and the second, much more broadly distributed family is called Fluc in bacteria , (formerly crcB ) and renamed FEX in fungi and Arabidopsis …”

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Two New Members of CsFEXs Couple Proton Gradients to Export Fluoride and Participate in Reducing Fluoride Accumulation in Low-Fluoride Tea Cultivars

Song

Hou

Guo

et al. 2020

J. Agric. Food Chem.

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The accumulation of fluoride in tea leaves from various cultivars exhibits significant differences. However, the molecular basis and mechanism remain largely unknown. Here, we reported that two genes of CsFEX (fluoride export genes in Camellia sinensis), CsFEX1 and CsFEX2, transport fluoride out of cells, alleviate the cellular fluoride toxin, and rescue the yeast mutant (FEX1ΔFEX2Δ) and Arabidopsis mutant (fex), as their efflux activities are coupled with proton gradients. Further analysis found that CsFEX1 and CsFEX2 localize to the plasma membrane both in yeast and Arabidopsis cells. CsFEX2 is more effective to reduce fluoride toxicity in yeast and Arabidopsis compared with CsFEX1 even at low pH. CsFEX2 induced by fluoride treatment is around tenfold higher in a low-fluoride cultivar (Yunkang 10) than that in a high-fluoride cultivar (Pingyang Tezaocha), suggesting that CsFEX2 possibly plays a critical role in reducing fluoride accumulation in tea leaves.

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Section: Materials and Methodssupporting

confidence: 91%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Two New Members of CsFEXs Couple Proton Gradients to Export Fluoride and Participate in Reducing Fluoride Accumulation in Low-Fluoride Tea Cultivars

Song

Hou

Guo

et al. 2020

J. Agric. Food Chem.

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Location affects fatty acid composition in Camellia sinensis cv Tieguanyin fresh leaves

Guo

Wang

et al. 2019

J Food Sci Technol

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Tieguanyin tea is a typical representative of oolong tea in China, and is famous for its orchid-like aroma. Fatty acids are one of the important precursors for aroma production. However, fatty acid contents and compositions in Tieguanyin largely remain undefined. In this study, we quantified the fatty acid composition in Tieguanyin and its offspring by gas chromatography-flame ionization detector, and compared the effects of growth sites and harvest time on the leaf fatty acid composition. The results showed that total fatty acid contents in Tieguanyin fresh leaves were higher than its offspring. Growth sites had significant impacts on fatty acid contents. Tieguanyin grown in Anxi County showed higher total fatty acid contents, and higher ratio of total unsaturated fatty acids to total saturated fatty acids. The fresh leaves in the morning showed higher total fatty acid contents compared to samples harvested in the afternoon or evening, suggesting a dynamic fatty acid degradation during day period.During tea processing, unsaturated fatty acids including linolenic acid, linoleic acid and oleic acid (18:1 D9c ) decreased 13.1%, 13.2% and 84.2%, respectively. The ratio of unsaturated fatty acids to saturated fatty acids still was above 300%. We found that Tieguanyin was a typical 18:3 plant, and the higher ratio of unsaturated fatty acids to saturated fatty acids of Tieguanyin grown in Anxi County may contribute to its characteristics aroma.

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Transcriptome-based variations effectively untangling the intraspecific relationships and selection signals in Xinyang Maojian tea population

Cheng

Wang

et al. 2023

Front. Plant Sci.

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As one of the world’s top three popular non-alcoholic beverages, tea is economically and culturally valuable. Xinyang Maojian, this elegant green tea, is one of the top ten famous tea in China and has gained prominence for thousands of years. However, the cultivation history of Xinyang Maojian tea population and selection signals of differentiation from the other major variety Camellia sinensis var. assamica (CSA) remain unclear. We newly generated 94 Camellia sinensis (C. sinensis) transcriptomes including 59 samples in the Xinyang area and 35 samples collected from 13 other major tea planting provinces in China. Comparing the very low resolution of phylogeny inferred from 1785 low-copy nuclear genes with 94 C. sinensis samples, we successfully resolved the phylogeny of C. sinensis samples by 99,115 high-quality SNPs from the coding region. The sources of tea planted in the Xinyang area were extensive and complex. Specifically, Shihe District and Gushi County were the two earliest tea planting areas in Xinyang, reflecting a long history of tea planting. Furthermore, we identified numerous selection sweeps during the differentiation of CSA and CSS and these positive selection genes are involved in many aspects such as regulation of secondary metabolites synthesis, amino acid metabolism, photosynthesis, etc. Numerous specific selective sweeps of modern cultivars were annotated with functions in various different aspects, indicating the CSS and CSA populations possibly underwent independent specific domestication processes. Our study indicated that transcriptome-based SNP-calling is an efficient and cost-effective method in untangling intraspecific phylogenetic relationships. This study provides a significant understanding of the cultivation history of the famous Chinese tea Xinyang Maojian and unravels the genetic basis of physiological and ecological differences between the two major tea subspecies.

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Variation of Photosynthesis, Fatty Acid Composition, ATPase and Acid Phosphatase Activities, and Anatomical Structure of Two Tea (Camellia sinensis (L.) O. Kuntze) Cultivars in Response to Fluoride

Cited by 5 publications

References 42 publications

Two New Members of CsFEXs Couple Proton Gradients to Export Fluoride and Participate in Reducing Fluoride Accumulation in Low-Fluoride Tea Cultivars

Two New Members of CsFEXs Couple Proton Gradients to Export Fluoride and Participate in Reducing Fluoride Accumulation in Low-Fluoride Tea Cultivars

Location affects fatty acid composition in Camellia sinensis cv Tieguanyin fresh leaves

Transcriptome-based variations effectively untangling the intraspecific relationships and selection signals in Xinyang Maojian tea population

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