Variation of fluorescence in tissue with temperature

Zaman, Raiyan T.; Rajaram, Narasimhan; Walsh, Alex J.; Oliver, Jeffrey W.; Rylander, H. G.; Tunnell, James W.; Welch, Ashley J.; Mahadevan‐Jansen, Anita

doi:10.1002/lsm.21023

Cited by 10 publications

(9 citation statements)

References 25 publications

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“…The fluorescence decrease observed in Figure 3 is similar to the trends reported by Menter 13, Buttemere et al 24, and Zaman et al 25. Menter 13 tested the reversibility of the temperature‐induced fluorescence loss in extracted collagen and found that the recovery exhibited hysteresis.…”

Section: Discussionsupporting

confidence: 85%

“…Lin et al 15 observed in liver tissue that the fluorescence intensity changed as the tissue temperature was increased during radio‐frequency ablation. Zaman et al 19 conducted a study to explore the effect of temperature on tissue fluorescence of porcine sclera in vitro , and hamster skin in vivo and showed that the fluorescence peak height decreased with increased temperature. This attempt to explore the temperature dependence of fluorescence did not examine the thermally induced changes in optical properties and how these optical property changes influence the fluorescence yield.…”

Section: Introductionmentioning

confidence: 99%

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The effect of temperature on the autofluorescence of scattering and non‐scattering tissue

et al. 2012

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

The effect of temperature on the autofluorescence of scattering and non‐scattering tissue

et al. 2012

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“…This is linked to a decrease in the fluorescence signal intensity on a pixel‐by‐pixel basis. Previously published results in enucleated pig eyes and hamster skin confirmed this observation (Zaman et al ., ). A different behaviour was observed at the cell membrane, where the temperature consistently appears to be lower, in accordance with the results shown in Figure .…”

Section: Resultsmentioning

confidence: 97%

Temperature measurement in the microscopic regime: a comparison between fluorescence lifetime‐ and intensity‐based methods

Paviolo

Clayton

McArthur

et al. 2013

Journal of Microscopy

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SummaryThermally sensitive fluorescent indicators have been proposed to monitor temperature changes in microfluidic systems, mainly based on fluorescence intensity or lifetime. However, measuring temperature in a structured environment, such as biological tissue, presents additional challenges due to the chemical and structural complexity. Here, we investigate the potential for resolving temperature distributions within the volume of a single cell. Rhodamine B (RhB) dye was employed as a temperature indicator to compare fluorescence intensity-and lifetime-based techniques. The relationship between the fluorescence lifetime and temperature was found to be highly dependent on the biological environment. The intensity-based method allowed the temperature distribution to be mapped with partial success within the volume of a single cell. Under ideal circumstances, the temperature can be mapped pixel by pixel with a resolution better than ±0.3• C within the cell cytoplasm, but this accuracy was reduced to ±1.8• C by environmental variations. These results suggest that the fluorophore should be encapsulated and immobilized in the biological tissue in order to reduce the influence of environmental factors on temperature measurements at the cellular level.

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“…For example, in lung tissue, steep variations in the experimental data are likely due to ex vivo tissue manipulations, such as dilution errors, large temperature variations, 18 bacterial contamination of some samples or intersample variations due to differential blood oxygenation or blood retention. The model autofluorescence predictions were previously validated under fully controlled experimental conditions (using liquid phantoms of known optical properties).…”

Section: Autofluorescence Versus Tissue Dilutionmentioning

confidence: 99%

Effect of tissue optics on wavelength optimization for quantum dot-based surface and subsurface fluorescence imaging

et al. 2012

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Abstract. Optimization is an important but relatively unexplored aspect of contrast-enhanced fluorescence imaging, since minimizing contrast agent usage reduces the associated cost and potential toxicity. In a previous study, the authors developed a quantitative experimental approach to optimize quantum dot (QD)-based imaging using homogenized liver as a model tissue. In this follow-up study, the authors further extend and validate the approach using eight different tissues and five QDs emission wavelengths, and introduce quantitative imaging performance metrics, namely the threshold QD concentration and wavelength optimization gain. These metrics allow quantification of the improvements through spectral optimization in terms of reduced QD dose and identify the conditions that make the optimization process worthwhile. The authors show that, for most tissues, the most important parameter to optimize is the emission wavelength, yielding improvements of up to four orders of magnitude, followed by the excitation wavelength (up to 20-fold improvement) and the excitation filter bandwidth (up to 50% improvement). The authors also observe, by means of the optimization gain metric, that tissues exhibiting both high autofluorescence and strong pigmentation are generally better candidates for excitation wavelength optimization. This work contributes to the development of robust and quantitative dosimetry for QD-based fluorescence imaging near to the tissue surface.

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Variation of fluorescence in tissue with temperature

Abstract: Cooling a tissue increased fluorescence intensity of skin in vivo, in all experiments. In vitro results were consistent with in vivo measurements.

Cited by 10 publications

References 25 publications

The effect of temperature on the autofluorescence of scattering and non‐scattering tissue

The effect of temperature on the autofluorescence of scattering and non‐scattering tissue

Temperature measurement in the microscopic regime: a comparison between fluorescence lifetime‐ and intensity‐based methods

Effect of tissue optics on wavelength optimization for quantum dot-based surface and subsurface fluorescence imaging

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