Variation of bcl-2 expression in breast ducts and lobules in relation to plasma progesterone levels: overexpression and absence of variation in fibroadenomas

Ferrières, Gaëlle; Cuny, Marguerite; Simony-Lafontaine, Joëlle; Jacquemier, Jocelyne; Rouleau, Caroline; Guilleux, F; Grenier, Jean; Rouanet, Philippe; Pujol, H; Jeanteur, Philippe; Escot, Chantal

doi:10.1002/(sici)1096-9896(199710)183:2<204::aid-path921>3.0.co;2-m

Cited by 24 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the different patterns of Bcl-2 expression in the first part of the menstrual cycle, the results from those two studies suggest that the regulation of Bcl-2 expression in breast tissue is hormone-dependent. Furthermore, as discussed by Ferrieres and colleagues [50], these results suggest a loss in the control of Bcl-2 by progesterone in diseases originating from epithelial lobular components.…”

Section: Discussionsupporting

confidence: 61%

“…It has been shown that the relative ratios of these various pro-apoptotic and anti-apoptotic members of the Bcl-2 family determine the ultimate sensitivity or resistance of cells to diverse apoptotic stimuli [46]. Ferrieres and colleagues [50] found a correlation between Bcl-2 expression and progesterone levels, with greater Bcl-2 expression in the follicular phase, diminishing as the menstrual cycle progresses. In contrast, Sabourin and colleagues [51] found that Bcl-2 was expressed preferentially in lobular epithelial cells, with maximal expression at the mid-cycle period and a sharp decrease at the end of the menstrual cycle.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle

Navarrete

Maier

Falzoni³

et al. 2005

Breast Cancer Res

View full text Add to dashboard Cite

Introduction During the menstrual cycle, the mammary gland goes through sequential waves of proliferation and apoptosis. In mammary epithelial cells, hormonal and non-hormonal factors regulate apoptosis. To determine the cyclical effects of gonadal steroids on breast homeostasis, we evaluated the apoptotic index (AI) determined by terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) staining in human mammary epithelial cells during the spontaneous menstrual cycle and correlated it with cellular proliferation as determined by the expression of Ki-67 during the same period.

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle

Navarrete

Maier

Falzoni³

et al. 2005

Breast Cancer Res

View full text Add to dashboard Cite

show abstract

“…bcl-2, however, is also overexpressed in a variety of other tumors that lack this typical chromosomal alteration (Lu et al, 1993a;Pezzella et al, 1993). In addition, the expression of bcl-2 seems to be under hormonal control in normal tissues (Tilly, 1996), where it participates in hormone-dependent tissue remodelling, and in breast, endometrial, and prostate cancers (Apakama et al, 1996;Ferrieres et al, 1997;Taskin et al, 1997). Finally, bcl-2 expression has been implicated in drug responsiveness, since the ability of some chemotherapeutic agents to induce phosphorylation of bcl-2 has been assumed as a mechanism by which they can suppress the anti-apoptotic activity of bcl-2, and thus promote cell death (Haldar et al, 1996).…”

mentioning

confidence: 99%

Analysis of SYT-SSX Fusion Transcripts and bcl-2 Expression and Phosphorylation Status in Synovial Sarcoma

Mancuso

Mezzelani

Riva

et al. 2000

Laboratory Investigation

View full text Add to dashboard Cite

SUMMARY:Synovial sarcomas (SS) are characterized by a chromosomal translocation t(X;18)(p11.2;q11.2) which usually fuses the SYT gene from chromosome 18 to SSX1 or SSX2 genes on chromosome X. Also, a variant SYT-SSX4 fusion gene has recently been shown in a single SS case. In addition to these cytogenetic changes, bcl-2 expression, as assessed by immunohistochemistry, has been reported to be an almost general constitutive alteration of SS. In the present work, we analyze a series of 36 SS surgical samples (from 34 patients) by RT-PCR for the presence of the SYT-SSX1 or the SYT-SSX2 fusion transcript. The analysis was extended to SYT-SSX4 on SYT-SSX1-negative and SYT-SSX2-negative cases only. Our results showed a significant correlation between the SYT-SSX2 fusion and the monophasic SS histologic subtype. SYT-SSX1 fusion transcripts were present in both monophasic and biphasic tumors. The SYT-SSX4 fusion type was detected in a single monophasic SS. In the same series of SS cases, we also confirmed and extended the previously reported constitutive expression of bcl-2 protein, by using both immunohistochemical and western blot analysis. Moreover, we demonstrated that the BCL-2 gene is not rearranged or amplified at genomic level, indicating that the high levels of bcl-2 expression observed in SS might result from transcriptional activation of the gene and/or protein stabilization. Finally, we show that bcl-2 is not phosphorylated in tumors from patients who had been preoperatively treated with radio/chemotherapy, in tumors from untreated patients, or in an SS cell line (CME-1) after in vitro treatment with cytotoxic concentrations of DNA-damaging agents or taxanes. These data indicate that SS cells are unable to activate an apoptosis pathway involving bcl-2 phosphorylation/inactivation and may provide a possible explanation for the limited effectiveness of conventional pharmacological treatments of this tumor type. (Lab Invest 2000, 80:805-813).

show abstract

“…Moreover, studies follow different procedures with regard to 'antigen exposing' and principles of incubation with antibodies, as well as in microscopic assessment criteria. Studies that analyzed the expression of ERs and other markers in breast cancer usually lacked information as to whether or not the cases analyzed included tumors without chemotherapy or tumors treated with pre-operative chemotherapy, and the extent thereof (20)(21)(22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

“…No relationship was observed between ER expression and Bak. Other studies have shown that estrogens increase Bcl-2 protein expression in epithelial breast cells (21) and in certain breast cancer lines, thereby lengthening the life of these cells (22). Berardo et al (23) showed that a high Bcl-2 expression was associated with favorable prognostic factors, such as positive ER status, a low fraction of cells in the S-phase of the cell cycle and a low number of lymph nodes with neoplastic metastases.…”

Section: Comparison Of Markers P-value R ----------------------------mentioning

confidence: 99%

ERα and ERβ expression in correlation with Ki-67, Bcl-2 and Bak in primary tumors and lymph node metastases of breast cancer: The effect of pre-operative chemotherapy

et al. 2010

View full text Add to dashboard Cite

Abstract. This study aimed to assess the pre-operative chemotherapy impact on the relationship between estrogen receptor (ER) expression and markers of proliferation and apoptosis in primary and metastatic breast cancer. Immunohistochemical examinations were conducted on surgically removed ductal invasive breast cancers and their lymph node metastases in 135 patients. A total of 64 patients from this group underwent pre-operative chemotherapy and in 71 cases the surgery was performed without primary chemotherapy. A negative correlation between ERα and Ki-67 was found in primary tumors and lymph node metastases. A positive correlation was observed between ERα and Bcl-2. A positive correlation was also noted between ERβ and Bak, suggesting that the two ERs were involved in the regulation of proteins responsible for the control of the apoptotic process. Assessment of the expression of the proteins conducted separately in primary tumors and lymph node metastases did not reveal a significant effect of pre-operative chemotherapy on the correlations of ERs with Ki-67, Bcl-2 and Bak. However, the analysis of the correlations between the receptor expression in primary tumors and Ki-67, Bcl-2 and Bak in lymph node metastases showed a statistically significant impact of pre-operative chemotherapy on the correlations of ERα and Bcl-2 with ERβ and Bak, confirming involvement of the two ERs in the regulation of apoptosis during breast carcinogenesis.

show abstract

Variation of bcl-2 expression in breast ducts and lobules in relation to plasma progesterone levels: overexpression and absence of variation in fibroadenomas

Cited by 24 publications

References 24 publications

Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle

Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle

Analysis of SYT-SSX Fusion Transcripts and bcl-2 Expression and Phosphorylation Status in Synovial Sarcoma

ERα and ERβ expression in correlation with Ki-67, Bcl-2 and Bak in primary tumors and lymph node metastases of breast cancer: The effect of pre-operative chemotherapy

Contact Info

Product

Resources

About