Variation in virulence of Beauveria bassiana and B. pseudobassiana to the pine weevil Pissodes nemorensis in relation to mycelium characteristics and virulence genes

Romón, Pedro; Hatting, Hardus; Goldarazena, Arturo; Iturrondobeitia, Juan Carlos

doi:10.1016/j.funbio.2016.11.008

Cited by 12 publications

(10 citation statements)

References 29 publications

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“…was shown to prevail on sites following soil disturbance [55][56][57]. Beauveria fungi can be important in controlling insect pests [58]. However, their natural occurrence and abundance in soils may depend on site disturbance as much higher relative abundances were reported in natural forest soils than in recent reforestations or agricultural soils [59].…”

Section: Discussionmentioning

confidence: 99%

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

et al. 2020

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The aim was to assess fungal communities associated with living needles and soil of Pinus sylvestris in managed and unmanaged forest stands to get a better understanding of whether and how different intensities of forest management affects fungal diversity and community composition under the north temperate forest zone conditions. The study was carried out in three national parks in Lithuania. Each included five study sites in managed stands and five in unmanaged stands. At each site, three random soil cores and five random last-year needle samples were collected. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput sequencing. Analysis of 195,808 high-quality reads showed the presence of 1909 fungal taxa. Richness and composition of fungal taxa were similar in each substrate (needles and soil) in managed vs. unmanaged sites. The most common fungi in needles were Coleosporium campanulae (12.4% of all fungal sequences), Unidentified sp. 3980_1 (12.4%), Unidentified sp. 3980_4 (4.1%) and Sydowia polyspora (3.1%). In soil: Unidentified sp. 3980_21 (8.6%), Umbelopsis nana (8.2%), Archaeorhizomyces sp. 3980_5 (8.1%) and Penicillium spinulosum (6.3%). The results demonstrated that managed and unmanaged P. sylvestris stands support similar diversity and composition of fungal communities associated with living needles and soil.

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Section: Discussionmentioning

confidence: 99%

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

et al. 2020

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“…There was considerable variation in aggressiveness among isolates, presenting isolate number 6 (ARSEF2518) the highest cumulative mortality value. It is interesting to note that some Coleoptera or Homoptera-derived isolates showed more aggressiveness than some isolates from Lepidoptera, supporting that the original host is not a reliable indicator of higher virulence in some entomopathogenic fungi (Varela & Morales, 1996;Devi et al, 2008;Romón et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Variability of Deltamethrin-Resistant <i>Metarhizium anisopliae</i> Aggressive Strains Group to a Population of the Cossid Moth from <i>Eucalyptus nitens</i>

Romón¹,

Hatting²,

Goldarazena³

et al. 2018

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Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.

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“…Also, the biochemical interactions during the infection process, i.e., secretion of chitinase by B. bassiana might have contributed to the range of variation in virulence as suggested previously [17]. Several research works have been done on the isolation, identification and purification of extra-cellular cuticle degrading enzymes from EPF [18,19]. However, the method described in this paper is of particular value for analysis of variation in virulence of entomopathogens using insect cuticles as substrate.…”

Section: Figurementioning

confidence: 90%

“…Several research works have been done on the isolation, identification and purification of extra‐cellular cuticle degrading enzymes from EPF . However, the method described in this paper is of particular value for analysis of variation in virulence of entomopathogens using insect cuticles as substrate.…”

Section: Quantification Of Chitinase Of B Bassiana (B2 and B10) Growmentioning

confidence: 99%

Analysis of variation in virulence of Beauveria bassiana against insect pests of pigeonpea using qPCR

et al. 2017

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Beauveria bassiana is a broad spectrum microbial bioagent used for the control of agriculturally important insect pests. However, in our experiments, two virulent isolates of B. bassiana (B2 and B10) showed specific preference toward Maruca vitrata and Helicoverpa armigera of pigeonpea. To better understand this feature, we developed a qPCR assay to quantify the chitinase (virulence factor) of B. bassiana during the infection process. Isolates of B. bassiana were grown on insect cuticle amended medium and minimal medium (without insect cuticle) to assess the induction of chitinase. Our results revealed a positive correlation between expression of chitinase by B. bassiana and the substrates (with or without cuticles of M. vitrata and H. armigera) used. This study showcases the methodology to quantify the chitinase and analysis of variation in virulence of B. bassiana (B2 and B10) against M. vitrata and H. armigera.

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Variation in virulence of Beauveria bassiana and B. pseudobassiana to the pine weevil Pissodes nemorensis in relation to mycelium characteristics and virulence genes

Cited by 12 publications

References 29 publications

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

Variability of Deltamethrin-Resistant <i>Metarhizium anisopliae</i> Aggressive Strains Group to a Population of the Cossid Moth from <i>Eucalyptus nitens</i>

Analysis of variation in virulence of Beauveria bassiana against insect pests of pigeonpea using qPCR

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Variation in virulence of Beauveria bassiana and B. pseudobassiana to the pine weevil Pissodes nemorensis in relation to mycelium characteristics and virulence genes

Cited by 12 publications

References 29 publications

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

Variability of Deltamethrin-Resistant &lt;i&gt;Metarhizium anisopliae&lt;/i&gt; Aggressive Strains Group to a Population of the Cossid Moth from &lt;i&gt;Eucalyptus nitens&lt;/i&gt;

Analysis of variation in virulence of Beauveria bassiana against insect pests of pigeonpea using qPCR

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Variability of Deltamethrin-Resistant <i>Metarhizium anisopliae</i> Aggressive Strains Group to a Population of the Cossid Moth from <i>Eucalyptus nitens</i>