Variation in total phenolics and antioxidant capacity among European plum genotypes

Rupasinghe, H.P. Vasantha; Subramanian, Jayasankar; Lay, W.

doi:10.1016/j.scienta.2006.01.020

Cited by 91 publications

(53 citation statements)

References 17 publications

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“…The correlation coefficient between the total phenolic content and free radical scavenging activity of DPPH and ABTS were r 2 = 0.970 and 0.978, respectively (see Figures 1 and 2). Quite a number of authors (Rupasinghe et al, 2006;Moyer et al, 2002;Jurikova and Matuskovic, 2007) refers to high correlation dependence of polyphenols and antioxidant activity in different fruit species. What is more interesting, the highest content of phenolic substances and antioxidant activity was measured in the cultivars ´Titus´ and ´Vydubeckij´, which are Russian in origin.…”

Section: Resultsmentioning

confidence: 99%

“…In its radical form, DPPH + absorbs light at 515 nm, but upon reduction by an antioxidant or a radical species, the absorption disappears (Thaipong et al, 2006). In both methods the calculated activity was converted using a calibration curve of the standard and expressed in ascorbic acid equivalents (AAE) (Rupasinghe et al, 2006).…”

Section: Total Phenolic Content (Tpc) and Free Radical Scavenging Assaymentioning

confidence: 99%

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Selected cultivars of cornelian cherry (Cornus mas L.) as a new food source for human nutrition

Rop¹,

Mlček²,

Kramářová³

et al. 2010

Afr. J. Biotechnol.

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The aim of this work was to determine antioxidant activity in 12 cultivars of cornelian cherry (Cornus mas L.). Two assays based on ion reduction of ABTS (2,2´-azinobis(3-ethylbenzothiazoline-6-sulphonate) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals were used for antioxidant activity. Total phenolic content of the fruit was analysed by Folin-Ciocelteau colorimetric method and ascorbic acid content was analysed using column chromatography -electrochemical detector (Coulochem III). The highest amounts of total phenolic content were found in cultivars ´Vydubeckij´ and ´Titus´ as 7.96 and 8.11 g gallic acid kg -1 of fresh mass (FM). High correlation between polyphenols and antioxidant activity in fruits of the cultivars was observed (r 2 = 0.970 for DPPH test and r 2 = 0.978 for ABTS test). The highest total content of ascorbic acid was determined in cultivar ´Olomoucky´, with the value of 3.11 g kg -1 FM. This study attempts to contribute to the knowledge of human nutritional properties of these cornelian cherry cultivars and may be useful for the evaluation of dietary information and further propagation of cultivation and utilization of this fruit in the world.

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Section: Resultsmentioning

confidence: 99%

Section: Total Phenolic Content (Tpc) and Free Radical Scavenging Assaymentioning

confidence: 99%

Selected cultivars of cornelian cherry (Cornus mas L.) as a new food source for human nutrition

Rop¹,

Mlček²,

Kramářová³

et al. 2010

Afr. J. Biotechnol.

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“…Antioxidant activity was calculated as a decrease in the absorbance value using the formula: (%)=(A0-A1/A0)×100%, where A0 is the absorbance of the control (without the sample), and A1 is the absorbance of the mixture containing the sample. The absorbance results were converted using a standard calibration curve and expressed in ascorbic acid equivalents (AAE) [26]. This protocol was repeated three times for each flower extract.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Edible flowers — antioxidant activity and impact on cell viability

Kuceková¹,

Mlček

Humpolíček³

et al. 2013

Open Life Sciences

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The phenolic compound composition, antioxidant activity and impact on cell viability of edible flower extracts of Allium schoenoprasum; Bellis perennis; Cichorium intybus; Rumex acetosa; Salvia pratensis; Sambucus nigra; Taraxacum officinale; Tragopogon pratensis; Trifolium repens and Viola arvensis was examined for the first time. Total phenolic content of the flowers of these plants fell between 11.72 and 42.74 mg of tannin equivalents/kg of dry matter. Antioxidant activity ranged from 35.56 to 71.62 g of ascorbic acid equivalents/kg of dry matter. Using the Human Hepatocellular Carcinoma cell-line (HepG2) and the Human Immortalized Non-tumorigenic Keratinocyte cell line (HaCaT), we assessed cell viability following a 3 day incubation period in media containing 25, 50, 75 and 100 μg/ml of total phenolic compounds using a colorimetric MTT assay. These three properties could make the herbs useful in treatment of various diseases like cancer. The tested extracts had significant effects on cell viability, but the effects were dependent not only on the phenolic compound concentration and the edible flowers species, but also on the phenolic compound and antioxidant profiles. In addition, responses differed between cell lines.

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“…Then the absorbance was taken at 515 nm. The antioxidant activity was calculated as a decrease in absorbance value using the equation: The results of absorbance were converted using a calibration curve of the standard and expressed in ascorbic acid equivalents (AAE) (Rupasinghe et al 2006). For comparison, the extract providing 50% of radical scavenging activity (IC 50 , the concentration of the sample to scavenge 50% of the DPPH radicals) was calculated from the graph of radical scavenging activity percentage against extract concentration.…”

Section: Antioxidant Activity By the Dpph Test Assaymentioning

confidence: 99%

Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit

Rop¹,

Řezníček²,

Mlček³

et al. 2011

Hortic. Sci.

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Rop O., Řezníček V., Mlček J., Juríková T., Balík J., Sochor J., Kramářová D., 2011. Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit. Hort. Sci. (Prague), 38: 63-70.The fruit of blue honeysuckle (Lonicera caerulea L. var. kamtchatica (Sevast.) Pojark.) belongs to the fruit of great value because of itsunique chemical composition. The aim of our measurement of this fruit species was to select the cultivars which could be the most suitable food supplement in relation to strengthening human immunity system. The comparison of the fruit of 12 cultivars was made. The highest contents of polyphenolic substances were recorded in the cultivar Zolushka with the value of 9.03 g of gallic acid/kg fresh mass. In particular cultivars the DPPH (2,2-diphenyl-1-picrylhydrazyl) test was performed to determine antioxidant activity which ranged from 6.59-10.17 g of ascorbic acid equivalent/kg fresh mass. For comparison, scavenging activity of reactive oxygen species (superoxide anion, hydroxyl radical, and nitric oxide) was determined by using a 25% methanolic extract of fruit of particular cultivars. Antioxidant potential was also assessed using the rat liver slice model. The highest values of scavenging activity were found in the cultivars Zolushka, Goluboe vreteno, and Gerda. The cultivars Zolushka, Goluboe vreteno, and Gerda had also high values of flavonoids. The presented results may be used when popularizing this fruit species and also when studying the properties of new food and genetic resources.

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Variation in total phenolics and antioxidant capacity among European plum genotypes

Cited by 91 publications

References 17 publications

Selected cultivars of cornelian cherry (Cornus mas L.) as a new food source for human nutrition

Selected cultivars of cornelian cherry (Cornus mas L.) as a new food source for human nutrition

Edible flowers — antioxidant activity and impact on cell viability

Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit

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