Variation in the stearoyl-CoA desaturase gene (&amp;lt;i&amp;gt;SCD&amp;lt;/i&amp;gt;) and its influence on milk fatty acid composition in late-lactation dairy cattle grazed on pasture

Li, Yunhai; Zhou, Huitong; Cheng, Long; Zhao, Jenny H.; Hickford, Jonathan G. H.

doi:10.5194/aab-63-355-2020

Cited by 13 publications

(7 citation statements)

References 30 publications

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“…The next interesting ROH hotspot region identified only by the HD panel was localized on chromosome 26. This region overlapped with the SCD gene that encodes the stearoyl-CoA desaturase enzyme, which was proposed in numerous studies (Li et al, 2020;Tian et al, 2022) to regulate milk fat synthesis and influence milk composition. Again, this signal was not detected in the case of the medium-density SNP panel.…”

Section: Roh Hotspots and Their Gene Contentmentioning

confidence: 99%

Comprehensive analysis of runs of homozygosity and heterozygosity in Holstein cattle on the basis of medium and high density SNP panels and large population sample

Szmatoła,

Gurgul,

Jasielczuk

et al. 2024

Annals of Animal Science

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This study reports runs of homozygosity (ROH) and heterozygosity (ROHet) distributed in a large population of Holstein cattle on the basis of two microarrays of medium (50k; 2163 animals; 54 609 SNPs) and high single nucleotide polymorphism (SNP) density (HD; 600 animals; 777 692 SNPs). To assess the inbreeding values of Holstein cattle, the ROH-based genomic inbreeding coefficient (FROH) was calculated. The comparison of SNP panels suggested that FROH values above 4 Mb should be considered for panels of medium densities as a relatively reliable measure of inbreeding. Moreover, ROH hotspots and coldspots were identified and compared between the HD and 50k SNP panels and were carefully examined for association with production and functional traits. The obtained results pinpointed genomic regions presumably under selection pressure in Holstein cattle. The regions overlapped with a large number of genes, including GHR, GBF1, SUMF1, CCL28, NIM1K, U6, BTRC and FABP1, many of which are involved in important Holstein cattle characteristics. We also found that some ROH hotspots and coldspots identified with the HD panel were not detected with the 50k panel, mainly because of insufficient SNP density in certain genomic regions. This suggests that using medium-density panels might not be the best choice when precise identification of ROH patterns is the main goal. In summary, in this work, we confirmed that a high-density SNP panel compared to a medium-density SNP panel allows for more precise identification of ROH patterns, especially in the case of short ROH that could be associated with ancestral inbreeding.

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Section: Roh Hotspots and Their Gene Contentmentioning

confidence: 99%

Comprehensive analysis of runs of homozygosity and heterozygosity in Holstein cattle on the basis of medium and high density SNP panels and large population sample

Szmatoła,

Gurgul,

Jasielczuk

et al. 2024

Annals of Animal Science

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“…Single nucleotide polymorphisms in the SCD1 locus showed a high correlation with high concentration of C10:1, C12:1, C14:1, and higher C18 index in milk fatty acid composition of dairy cows . The nucleotide substitutions of SCD1 was associated with MUFAs, and it could be used as gene markers for improving animal breeding . Subacute ruminal acidosis of dairy cattle downregulated SCD1 expression through epigenetic regulation .…”

Section: Introductionmentioning

confidence: 97%

“…10 The nucleotide substitutions of SCD1 was associated with MUFAs, and it could be used as gene markers for improving animal breeding. 11 Subacute ruminal acidosis of dairy cattle downregulated SCD1 expression through epigenetic regulation. 12 In goat mammary epithelial cells (GMECs), SCD1 was shown to promote the synthesis of cellular triacylglycerol (TAG).…”

Section: ■ Introductionmentioning

confidence: 99%

Knockout of Stearoyl-CoA Desaturase 1 Decreased Milk Fat and Unsaturated Fatty Acid Contents of the Goat Model Generated by CRISPR/Cas9

Tian

Niu

Luo

et al. 2022

J. Agric. Food Chem.

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Goat milk contains a rich source of nutrients, especially unsaturated fatty acids. However, the regulatory mechanism of milk fat and fatty acid synthesis remains unclear. Stearoyl-CoA desaturase 1 (SCD1) is the key enzyme catalyzing monounsaturated fatty acid synthesis and is essential for milk lipid metabolism. To explore milk lipid synthesis mechanism in vivo, SCD1-knockout goats were generated through CRISPR/Cas9 technology for the first time. SCD1 deficiency did not influence goat growth or serum biochemistry. Plasma phosphatidylcholines increased by lipidomics after SCD1 knockout in goats. Whole-blood RNA-seq indicated alterations in biosynthesis of unsaturated fatty acid synthesis, cAMP, ATPase activity, and Wnt signaling pathways. In SCD1-knockout goats, milk fat percentage and unsaturated fatty acid levels were reduced but other milk components were unchanged. Milk lipidomics revealed decreased triacylglycerols and diacylglycerols levels, and the differential abundance of lipids were enriched in glycerolipid, glycerophospholipids, and thermogenesis metabolism pathways. In milk fat globules, the expression levels of genes related to fatty acid and TAG synthesis including SREBP1 were reduced. ATP content and AMPK activity were promoted, and p-p70S6K protein level was suppressed in SCD1-knockout goat mammary epithelial cells, suggesting that SCD1 affected milk lipid metabolism by influencing AMPK-mTORC1/p70S6K-SREBP1 pathway. The integrative analysis of gene expression levels and lipidomics of milk revealed a crucial role of SCD1 in glycerolipids and glycerophospholipids metabolism pathways. Our observations indicated that SCD1 regulated the synthesis of milk fat and unsaturated fatty acid in goat by affecting lipid metabolism gene expression and lipid metabolic pathways. These findings would be essential for improving goat milk nutritional value which is beneficial to human health.

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“…The SCD1 gene encodes a protein of 359 amino acids (Kęsek et al, 2017). The SCD1 gene is primary expressed in adipose tissue and mammary gland in farm animals (Li et al, 2020). The SCD1 gene encodes an enzyme involved in the biosynthesis of fatty acids, primarily oleic acid (Bernard et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic Polymorphisms of SCD1 gene and its relationship with some reproductive traits in Holstein cows in Iraq

Khaleel,

Abdullah,

Omear

2023

TJAS

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The research was conducted at the Khalis cows station in Al-Khalis district - Diyala Governorate and the central laboratory of the Faculty of Agriculture - University of Tikrit for the period from 13/12/2020 to 1/2/2022, a sample of 63 Holstein cows, with the aim of extracting the genetic material and determining the genetic polymorphism of the SCD1 gene and its relationship to reproductive traits in Holstein cows, studying the percentage of distribution of genetic structures in the cows that were studied, the allele frequency and calculating the value of chi-square (χ2) were determined. The genotypes frequencies for the SCD1 gene in the studied cow samples were 47.62, 46.03 and 6.35% for AA, AV and VV genotypes respectively. The value of the chi-square (χ2) was highly significant (P < 0.01). The frequency of allele A and V were 0.71 and 0.29 respectively. Some reproductive traits were significantly affected by the different genotypes of the SCD1 gene. Cows with the genotype AA achieved the highest Days Open (106.70 ± 5.84 days), while the genotypes VV and AV were 105.93 ± 14.25 and 92.82 ± 5.89 days respectively. The cows with AA genotype was the highest calving interval (389.77 ± 5.90 days), while the cows of the VV and AV genotypes reached 386.75 ± 14.39 and 374.27 ± 5.96 days respectively. It can be concluded by studying the genetic polymorphism of the SCD1 gene that it is possible to improve the reproductive traits in cows. We also recommend applying the study to a larger sample for several seasons and different locations of the gene, while studying more economic characteristics to give more accurate results for the application of the selection and exclusion strategy.

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Variation in the stearoyl-CoA desaturase gene (<i>SCD</i>) and its influence on milk fatty acid composition in late-lactation dairy cattle grazed on pasture

Cited by 13 publications

References 30 publications

Comprehensive analysis of runs of homozygosity and heterozygosity in Holstein cattle on the basis of medium and high density SNP panels and large population sample

Comprehensive analysis of runs of homozygosity and heterozygosity in Holstein cattle on the basis of medium and high density SNP panels and large population sample

Knockout of Stearoyl-CoA Desaturase 1 Decreased Milk Fat and Unsaturated Fatty Acid Contents of the Goat Model Generated by CRISPR/Cas9

Genetic Polymorphisms of SCD1 gene and its relationship with some reproductive traits in Holstein cows in Iraq

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