Variation in the salivary proteomes of differentially virulent greenbug (Schizaphis graminum Rondani) biotypes

Nicholson, Scott J.; Puterka, Gary J.

doi:10.1016/j.jprot.2013.12.005

Cited by 57 publications

(60 citation statements)

References 82 publications

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“…Proteome analysis of the pea aphid salivary glands and saliva, collected in vitro in artificial diets, from a number of aphid species identified a large number of secreted proteins and demonstrating the diverse constituents of aphid saliva (Harmel et al ; Carolan et al , ; Cooper et al , ; Nicholson et al ; Rao et al ; Chaudhary et al , ; Nicholson and Puterka ; Vandermoten et al ). A few key findings from these salivary protein studies have been revealed.…”

Section: Hemipteran and Dipteran Effectorsmentioning

confidence: 99%

Hemipteran and dipteran pests: Effectors and plant host immune regulators

Kaloshian

Walling

2015

JIPB

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Hemipteran and dipteran insects have behavioral, cellular and chemical strategies for evading or coping with the host plant defenses making these insects particularly destructive pests worldwide. A critical component of a host plant's defense to herbivory is innate immunity. Here we review the status of our understanding of the receptors that contribute to perception of hemipteran and dipteran pests and highlight the gaps in our knowledge in these early events in immune signaling. We also highlight recent advances in identification of the effectors that activate pattern-triggered immunity and those involved in effector-triggered immunity.

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Section: Hemipteran and Dipteran Effectorsmentioning

confidence: 99%

Hemipteran and dipteran pests: Effectors and plant host immune regulators

Kaloshian

Walling

2015

JIPB

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“…The other common proteins belong to the GMC-oxidoreductase family and these are the most frequently reported bioactive proteins in studies of aphid saliva detected either by substrate-specifi c assays (Madhusudhan and Miles 1998 ) or by direct identifi cation using mass spectrometry (Harmel et al 2008 ;Carolan et al 2009 ;Nicholson et al 2012 ;Rao et al 2013 ;Vandermorten et al 2014 ;Nicholson and Puterka 2014 ;see Table 1 ). GMC oxidoreductase in insect saliva in general has been implicated in the modifi cation of plant defence mechanisms (Eichenseer et al 1999 ;Musser et al 2002 ;2005 ), and in aphids specifi cally is speculated to be involved in the detoxifi cation of noxious phytochemicals and in promoting the gelling of sheath saliva by enhancing disulphide bridge formation (Miles and Oertli 1993 ).…”

Section: Composition Of Aphid Salivamentioning

confidence: 99%

“…2 . Interestingly, glucose dehydrogenase, another member of the GMC-oxidoreductase family, was also detected in the saliva of Diuraphis noxia and Schizaphis graminum , two pests of cereals in the USA that cause phytotoxic lesions following feeding (Nicholson et al 2012 ;Nicholson and Puterka 2014 ). Glucose dehydrogenase was the only signifi cant protein in the watery saliva of S. graminum in common with other aphid salivary proteomes (Nicholson and Puterka 2014 ), but analysis of the saliva from virulent and avirulent strains of this notorious pest suggested that the protein composition of the saliva might be an important factor in determining host plant responses to aphid feeding.…”

Section: Composition Of Aphid Salivamentioning

confidence: 99%

Proteomic Insights into the Hidden World of Phloem Sap Feeding

Khan¹,

Carolan

Wilkinson

2016

Management of Insect Pests to Agriculture

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The physical interface between a phloem-feeding insect and its host plant is a single cell buried deep within the plant tissue. As such, the molecular interactions between these notorious agricultural pests and the crop plants upon which they feed are diffi cult to study. 'Omic' technologies have proved crucial in revealing some of the fascinating detail of the molecular interplay between these partners. Here we review the role of proteomics in identifying putative components of the secreted saliva of phloem-feeding insects, particularly aphids, and discuss the limited knowledge concerning the function of these proteins. OverviewPhloem feeding insects represent a guild of agricultural pests that are notoriously diffi cult to study and even harder to control (van Emden and Harrington 2007 ). Much of the problem lies in the location of the feeding site since the physical interface between the insect and plant is a single sieve element cell within the phloem bundle buried deep in the leaf. As a consequence, ingestion of the diet cannot be observed directly as is possible in most chewing insects. Several approaches have been developed over the last few decades to address these issues and some notable achievements include the electrical penetration graph and the use of non-persistent plant viruses to determine the sequence of feeding behaviours between plant surface penetration and phloem sap ingestion (see Powell et al. 2006 for full review).

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“…Nicholson et al (2012) suggested that aphid-plant interactions are regulated by general proteins fed by aphids. Some aphid species, like Acyrthosiphon pisum Harris and Myzus persicae Sulzer, do not induce direct or immediate phytotoxic effects on plants (Nicholson & Puterka, 2014). The salivary proteins of these non-phytotoxic aphids mainly serve to destabilize general plant defense responses (Miles, 1999;Cherqui & Tjallingii, 2000;Tjallingii, 2006;Will et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Based on our personal observations, some damage to amaranth is related to A. craccivora phytotoxicity. Schizaphis graminum Rondani and Diuraphis noxia Kurdjumov are phytotoxic aphids that are also pests of wheat and other cereals that cause major economic damage (Nicholson et al, 2012;Nicholson & Puterka, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cowpea aphid–plant interactions: endosymbionts and related salivary protein patterns

Loudit

Bauwens

Francis

2018

Entomologia Exp Applicata

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The specificity of plant use by aphids is related to symbiont diversity of some aphid models. Aphis craccivora Koch (Hemiptera: Aphididae, Aphidini) is a well‐known aphid that feeds on species of Fabaceae, but has also been recorded recently on Amaranthus species (Amaranthaceae) in Gabon (Africa). Aphis craccivora strains used in this study were originally collected from crop Vigna unguiculata L. Walp. (Fabaceae) from Togba in Benin (Africa) and Amaranthus hybridus L. from Libreville in Gabon, for a comparative study of symbionts. Saliva composition, potentially including bacterial proteins, also contributes to the phytotoxic effect of aphid attacks. Both, endosymbiont bacteria and saliva protein diversity should be targeted to investigate the feeding behavior of aphids and to explain plant–aphid interactions. Bacteria‐targeted PCR was conducted on six symbionts in A. craccivora. The obligate symbiont Buchnera aphidicola Munson et al. (Enterobacteriaceae) was identified in all aphids collected. In comparison, the facultative symbiont Serratia symbiotica Moran et al. (Enterobacteriaceae) was only found in A. craccivora from Gabon, whereas Rickettsia sp. (Rickettsiaceae) was only found in aphids from Benin. Using nano‐LC‐MS/MS (liquid chromatography‐tandem mass spectrometry), some proteins were only found in solid or soluble saliva, whereas others originated from S. symbiotica. Two of the identified proteins are involved in plant–pathogen interactions: calmodulin and elongation factor Tu. This information on endosymbionts and related salivary proteomes from A. craccivora in Gabon helps improve our understanding of aphid–plant interactions.

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Variation in the salivary proteomes of differentially virulent greenbug (Schizaphis graminum Rondani) biotypes

Cited by 57 publications

References 82 publications

Hemipteran and dipteran pests: Effectors and plant host immune regulators

Hemipteran and dipteran pests: Effectors and plant host immune regulators

Proteomic Insights into the Hidden World of Phloem Sap Feeding

Cowpea aphid–plant interactions: endosymbionts and related salivary protein patterns

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