Variation in the promoter of the sorbitol dehydrogenase gene <i>MdSDH2</i> affects binding of the transcription factor MdABI3 and alters fructose content in apple fruit

Wang, Zhengyang; Ma, Baiquan; Yang, Nanxiang; Jin, Ling; Wang, Lan; Ma, Songya; Ruan, Yong‐Ling; Ma, Fengwang; Li, Mingjun

doi:10.1111/tpj.15624

Cited by 17 publications

(20 citation statements)

References 54 publications

(104 reference statements)

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“…Promoters are gene switches, located upstream of gene coding regions, and contain specific cis -acting elements that play a crucial role in gene transcription and expression [ 52 , 53 ]. The main active position of cis -acting elements with a biological function is 50 bp upstream of the transcription start sites (TSS), and most transcription factor binding sites (TFBS) are in the region of −1000 bp to +200 bp relative to the TSS [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Wang

Lei

et al. 2022

Genes

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The MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family plays an important role in plant growth, development, and response to biotic and abiotic stresses. However, the gene functions of MYB transcription factors in sweet potato (Ipomoea batatas (L.) Lam) have not been elucidated. In this study, an MYB transcription factor gene, IbMYB308, was identified and isolated from sweet potato. Multiple sequence alignment showed that IbMYB308 is a typical R2R3-MYB transcription factor. Further, quantitative real-time PCR (qRT-PCR) analysis revealed that IbMYB308 was expressed in root, stem, and, especially, leaf tissues. Moreover, it showed that IbMYB308 had a tissue-specific profile. The experiment also showed that the expression of IbMYB308 was induced by different abiotic stresses (20% PEG-6000, 200 mM NaCl, and 20% H2O2). After a 200 mM NaCl treatment, the expression of several stress-related genes (SOD, POD, APX, and P5CS) was upregulation in transgenic plants, and the CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. In conclusion, this study demonstrated that IbMYB308 could improve salt stress tolerance in transgenic tobacco. These findings lay a foundation for future studies on the R2R3-MYB gene family of sweet potato and suggest that IbMYB308 could potentially be used as an important positive factor in transgenic plant breeding to improve salt stress tolerance in sweet potato plants.

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Section: Discussionmentioning

confidence: 99%

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Wang

Lei

et al. 2022

Genes

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“…GWAS for soluble sugar contents showed that a SNP mutation of the main-effect gene MdSOT2 significantly reduced accumulation of sorbitol in apple fruit ( Liao et al, 2021 ). In addition, a stable QTL was detected in LG01 in “Honeycrisp.” The SNP A/G variation of the sorbitol dehydrogenase gene MdSDH2 promoter affects its binding to the TF MdABI3 , thereby downregulating the expression level of MdSDH2 and reducing the fructose content in the fruit ( Wang Z. et al, 2022b ). Thus, SNP mutations in genes that encode key enzymes for sugar synthesis have significant effects on the accumulation of various soluble sugars.…”

Section: Flavor and Nutritional Quality Of Apple Fruitmentioning

confidence: 99%

Research Progress on Genetic Basis of Fruit Quality Traits in Apple (Malus × domestica)

et al. 2022

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Identifying the genetic variation characteristics of phenotypic traits is important for fruit tree breeding. During the long-term evolution of fruit trees, gene recombination and natural mutation have resulted in a high degree of heterozygosity. Apple (Malus × domestica Borkh.) shows strong ecological adaptability and is widely cultivated, and is among the most economically important fruit crops worldwide. However, the high level of heterozygosity and large genome of apple, in combination with its perennial life history and long juvenile phase, complicate investigation of the genetic basis of fruit quality traits. With continuing augmentation in the apple genomic resources available, in recent years important progress has been achieved in research on the genetic variation of fruit quality traits. This review focuses on summarizing recent genetic studies on apple fruit quality traits, including appearance, flavor, nutritional, ripening, and storage qualities. In addition, we discuss the mapping of quantitative trait loci, screening of molecular markers, and mining of major genes associated with fruit quality traits. The overall aim of this review is to provide valuable insights into the mechanisms of genetic variation and molecular breeding of important fruit quality traits in apple.

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“…The apple fruit, as a heterotrophic organ, derives its sugar source mainly from sorbitol (accounts for ∼60 to 80%) and sucrose produced by leaf photosynthesis. 1,2 When sorbitol synthesis is inhibited in the leaves, more sucrose flows into the fruit from the phloem, keeping the total sugars in the fruit constant. 3 Sucrose is mainly synthesized in the mesophyll cells of the leaves.…”

Section: Introductionmentioning

confidence: 99%

Plasma Membrane-Localized Transporter MdSWEET12 Is Involved in Sucrose Unloading in Apple Fruit

Zhang

Wu³

et al. 2022

J. Agric. Food Chem.

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Sugar content is an important factor determining the flavor in apple fruit. Sugar unloading is a prerequisite step for sugar accumulation. However, little is known about sugar unloading mechanisms in apple. Transcriptomic sequencing of two apple varieties, "Envy" and "Pacific Rose," with significantly different sugar content was performed. MdSWEET12a from the SWEET transporter family was differentially expressed. Further study of the MdSWEET12a showed that this plasma membrane-localized transporter protein-encoding gene was mainly expressed in sieve element-companion cells (SE-CC) in the fruit, which was positively correlated with the sucrose accumulation during the development of "Envy" apple. Consistently manipulating the gene expression through either transient overexpression or silencing significantly increased or decreased the sugar content in apple fruit, respectively. Complementary growth experiments in mutant yeast cells indicated that MdSWEET12a transported sucrose. Heterologous expression of MdSWEET12a in tomato increased the expression of genes related to sugar metabolism and transport, leading to increased sugar content. These findings underpin the involvement of MdSWEET12a in sugar unloading in apple fruit.

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Variation in the promoter of the sorbitol dehydrogenase gene MdSDH2 affects binding of the transcription factor MdABI3 and alters fructose content in apple fruit

Cited by 17 publications

References 54 publications

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Research Progress on Genetic Basis of Fruit Quality Traits in Apple (Malus × domestica)

Plasma Membrane-Localized Transporter MdSWEET12 Is Involved in Sucrose Unloading in Apple Fruit

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